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Citations for Invivogen :
251 - 300 of 562 citations for 5 Fluoro 2 piperidin 2 yl 1H benzimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2022Quote: 2 × 106 pleura cells were plated and stimulated with 1 µg/mL poly(I:C) (Invivogen, San Diego, CA, USA) for 4 h at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-coated with laminin for 24 hr prior to treatment with MCC950 (1 μM, 2 hr, Invivogen, inh-mcc) followed by 15 min exposure to isoproterenol (100 nM) ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured for 2 weeks in the presence of 1 µg/mL puromycin (ant-pr-1, InvivoGen). The cells were single-cell-cloned using a limiting dilution method ...
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bioRxiv - Genomics 2022Quote: ... After 2 to 6 minutes of gentle manual shaking in ice cold PBS with 1% (v/v) primocin (InvivoGen), the intestinal pieces were inspected microscopically (x100 magnification ...
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bioRxiv - Immunology 2023Quote: ... of filtered PAO1 supernatant or TSB and KGMTM-2 containing purified flagellin at 1μg/ml or vehicle (tlrl-pafla; from P. aeruginosa; InvivoGen) were used for treating hTCEpi cells for various durations as indicated in the figure legends.
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bioRxiv - Immunology 2023Quote: ... or RPMI1640 (ATCC) supplemented with 10% FBS and 2 mM L-Gln (MC38) and 4 µg/mL Blasticidin (InvivoGen) (MC38-Her2-B2m-/-) ...
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bioRxiv - Molecular Biology 2019Quote: ... livers of 10-week-old male or female mice were harvested 6 hours after tail-vein injection of LPS (2 mg/kg, Invivogen) or vehicle (PBS).
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bioRxiv - Microbiology 2020Quote: ... A total of five adjuvants were tested for their efficacy within the vaccine formulations: Aluminum hydroxide gel (alum) (Alhydrogel adjuvant 2%, InvivoGen), Polyinosinic-polycytidylic acid (poly (I:C ...
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bioRxiv - Cancer Biology 2021Quote: We transduced cells with lentivirus carrying KRAB-dCas9 and sgRNA targeting candidate progrowth enhancers and performed puromycin (2 μg/ml) (InvivoGen) for three days postelectroporation to select against non-transduced cells ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Genetics 2019Quote: ... The untransduced cells and serially diluted virus-treated cells were cultured in the presence of 2 μg/ml puromycin or 20 μg/ml blasticidin S (InvivoGen). When almost all of the untransduced cells had died ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... In some cases, cells were first pre-treated for 1 h with following inhibitors (2 µM H-151, 10 µM MCC950 (InvivoGen)) before addition of the treatments ...
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bioRxiv - Immunology 2023Quote: ... the cells were pre-incubated with the respective antagonist as suggested by the manufacturer’s protocol (STING: H-151 for 1-2 hours and cGAS: RU.521 for 3 hours, both InvivoGen, USA) in cell growth medium ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 µg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 μg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed bone marrow cells were cultured in RPMI supplemented with 10% FBS with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were expanded to a surface-treated T-75 flask and treated with 2 µg/mL of puromycin (Invivogen) for 2 weeks for selection of the transduced cells to generate the cell line expressing hM3D(Gq) ...
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bioRxiv - Genomics 2024Quote: ... A total of 17,114 BFP+ cells were replated into a half deep 96 well plate (Applikon Biotechnology) and allowed to expand with the addition of 2 μg/mL doxycycline and 100 μg/mL blasticidin (Invivogen) to select for functional landing pad cells ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs at day 60 of differentiation were treated with 1-2 µM pevonedistat (Hycultec) or 750 ng MG-132 (InvivoGen) for three days ...
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bioRxiv - Immunology 2020Quote: ... LPS (5 µg/ml) or LPS (5 µg/ml) + concanavalin A (5 μg/ml; InvivoGen) were employed as control stimulants for splenocytes and isolated cells respectively.
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/uL primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in E14 standard medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... where n=2) were immunized with the indicated immunogen in 100 μL of 50% v/v of AddaVax™ adjuvant (Invivogen) and boosted with adjuvant on Day 14 ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Molecular Biology 2022Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/µl primorcin (InvivoGen, ant-pm-1) and 10 µM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... treated with CM 1:1 diluted in fresh cell growth media or PC (positive control: 1 μg/ml TLR-2 ligand Pam3CSK4 and 100 nM TLR-9 ligand CpG ODN 1668, both InvivoGen, USA). For experiments with different glucose concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-ppp-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (Catalog Code, lyec-12, InvivoGen), following the manufacturer’ ss instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...