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Citations for Invivogen :
401 - 450 of 464 citations for 3 7 Diethyl 2 hydrazinoquinoline hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... In some cases, cells were first pre-treated for 1 h with following inhibitors (2 µM H-151, 10 µM MCC950 (InvivoGen)) before addition of the treatments ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs at day 60 of differentiation were treated with 1-2 µM pevonedistat (Hycultec) or 750 ng MG-132 (InvivoGen) for three days ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were expanded to a surface-treated T-75 flask and treated with 2 µg/mL of puromycin (Invivogen) for 2 weeks for selection of the transduced cells to generate the cell line expressing hM3D(Gq) ...
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bioRxiv - Genomics 2024Quote: ... A total of 17,114 BFP+ cells were replated into a half deep 96 well plate (Applikon Biotechnology) and allowed to expand with the addition of 2 μg/mL doxycycline and 100 μg/mL blasticidin (Invivogen) to select for functional landing pad cells ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 µg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 μg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed bone marrow cells were cultured in RPMI supplemented with 10% FBS with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Immunology 2023Quote: ... followed by the medium being replaced with fresh IFNγ (5 ng/ml) containing media for 2 h before treatment with indicated stimulations: MDP (100 μM, Invivogen), iE-DAP (100 μM ...
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bioRxiv - Genetics 2023Quote: ... 5% CO 2 prior to stimulation under the same conditions with the following reagents and concentrations - Pam3Csk4 (Invivogen, Lot no.), LPS (Invivogen ...
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bioRxiv - Immunology 2024Quote: ... FL-BMDC cultures (2×105 cells per well) were stimulated with either 100 ng/ml profilin or 1 μM CpG-B/ODN1668 (Invivogen), or left unstimulated as a control in 200 ul DC medium in 96-well plates ...
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bioRxiv - Cell Biology 2024Quote: The transfected MDCK cells were selected using 400 μg/ml G418 disulfate (#08973-14, NacalaiTesque) or 200 μg/ml hygromycin B Gold (#ant-hg-2, Invivogen), the mixed clones with the lowest EGFP expression were collected by a FACS (SH800Z ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/uL primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in E14 standard medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... where n=2) were immunized with the indicated immunogen in 100 μL of 50% v/v of AddaVax™ adjuvant (Invivogen) and boosted with adjuvant on Day 14 ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Immunology 2023Quote: ... treated with CM 1:1 diluted in fresh cell growth media or PC (positive control: 1 μg/ml TLR-2 ligand Pam3CSK4 and 100 nM TLR-9 ligand CpG ODN 1668, both InvivoGen, USA). For experiments with different glucose concentrations ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/µl primorcin (InvivoGen, ant-pm-1) and 10 µM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-ppp-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (Catalog Code, lyec-12, InvivoGen), following the manufacturer’ ss instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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RORγt-dependent antigen-presenting cells direct regulatory T cell-mediated tolerance to food antigenbioRxiv - Immunology 2024Quote: ... with intraperitoneal injections of 100 μg of OVA mixed with 1 mg of Alum (1:1; Alhydrogel® adjuvant 2%; vac-alu-50; InvivoGen). Seven days after the final sensitization ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Pathology 2020Quote: ... FIV was diluted in PBS to 133μg/mL Coronavirus-specific protein and mixed 1:1 in 2% Alhydrogel (Invivogen vac-alu-250) to give a final concentration of 66.7μg/mL in 1% Alhydrogel ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Cell Biology 2023Quote: ... and if required relevant selection antibiotics (hygromycin 200 µg/ml ; InvivoGen, ant-hg-1 ; puromycin 2 µg/ml, InvivoGen, ant-pr-1). Im95m cells were routinely maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Neuroscience 2024Quote: ... which was composed of DMEM (Thermo, 41966) containing 10% Heat-inactivated FBS (HI-FBS, Cytiva, SH30071.03HI) and 2% primocin (Invivogen, ant-pm-1). The next day ...
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bioRxiv - Biochemistry 2024Quote: ... CD8+ T cell depletion was done for one treatment group (targeted HC-HAdV5HVR7, encoding for li-OVA.1+2 + muIL2v) with 0.2 mg mCD8-mIgG2 (InvivoGen 10354-42-01) via intraperitoneal injection per mouse and application ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...