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Citations for Invivogen :
351 - 394 of 394 citations for Gins Complex Subunit 2 Psf2 Homolog Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Eluted fractions were analyzed by western blotting with a mouse monoclonal RBD-specific antibody (InvivoGen, Cat# srbdmab10). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
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bioRxiv - Immunology 2021Quote: ... MCA-OVA tumor bearing mice were treated with 200 µg of anti-mCTLA4-mIgG2a monoclonal antibody (Invivogen) or 200 µg of isotype control antibody (mouse IgG2a ...
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bioRxiv - Immunology 2023Quote: The agonistic activity of anti-CD40 monoclonal antibodies was tested by stimulating HEK-Blue-CD40L™ (Invivogen) cells which harbor an NF-κB-inducible Secreted Embryonic Alkaline Phosphatase (SEAP ...
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bioRxiv - Immunology 2024Quote: ... mouse TLR2 neutralising antibody monoclonal mouse IgG2a (C9A12) and the control isotype mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralising antibody (monoclonal rat IgGk clone MTS510 ...
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bioRxiv - Cell Biology 2022Quote: ... the cationic lipid-based transfection reagent LyoVec and cyclic [G(2’,5’)pA(3’,5’)p] (2’3’-cGAMP) were obtained from Invivogen (San Diego, USA) and Lipofectamine2000 was obtained from ThermoFisher Scientific (Dreieich ...
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bioRxiv - Immunology 2022Quote: ... for 3 hr followed by (flagellin isolated from P. aeruginosa, 2 or 5 µg/mL) for 3 hr using FLA-PA Ultrapure (InvivoGen, tlrl-pafla) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Pathology 2020Quote: ... FIV was diluted in PBS to 133μg/mL Coronavirus-specific protein and mixed 1:1 in 2% Alhydrogel (Invivogen vac-alu-250) to give a final concentration of 66.7μg/mL in 1% Alhydrogel ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Cell Biology 2023Quote: ... and if required relevant selection antibiotics (hygromycin 200 µg/ml ; InvivoGen, ant-hg-1 ; puromycin 2 µg/ml, InvivoGen, ant-pr-1). Im95m cells were routinely maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Biochemistry 2024Quote: ... CD8+ T cell depletion was done for one treatment group (targeted HC-HAdV5HVR7, encoding for li-OVA.1+2 + muIL2v) with 0.2 mg mCD8-mIgG2 (InvivoGen 10354-42-01) via intraperitoneal injection per mouse and application ...
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bioRxiv - Neuroscience 2024Quote: ... which was composed of DMEM (Thermo, 41966) containing 10% Heat-inactivated FBS (HI-FBS, Cytiva, SH30071.03HI) and 2% primocin (Invivogen, ant-pm-1). The next day ...
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the targeting vectors and incubated for 2 days before addition of selective medium containing 1.5 μg/ml puromycin (Invivogen, cat# ant-pr-1). After 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Microbiology 2024Quote: ... low-passage vials of cells were thawed and incubated in Corning Falcon 75 cm2 vented culture flasks at a density of 2 x 106 / 15 mL of DMEM with 100 µg/mL Normocin (InvivoGen catalog # ant-nr) for approximately 72 hours ...
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bioRxiv - Biochemistry 2024Quote: Viral dsRNA-induced infection in cells was mimicked by treating the cells with 2 µg/mL of poly(I:C) LMW (InvivoGen, Cat. Code: tlrl-picw) dissolved in sterile PBS ...
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bioRxiv - Microbiology 2021Quote: ... Eluted fractions were analyzed by western blotting with a mouse monoclonal RBD specific antibody (InvivoGen, Cat# srbd-mab10). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
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bioRxiv - Plant Biology 2021Quote: ... Transgenic Col-0 (LUC) plants were selected on 1/2 MS medium supplemented with 50 μM hygromycin B (InvivoGen, Cat No. ant-hg-5). LUC activity was confirmed as described above ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... and MHC-I blocking antibody (W6/32; 50 ug/ml) in RPMI supplemented with human serum and primocin (Invivogen). Cells were harvested and stained with anti-CD3 FITC antibody (UCHT1 ...
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bioRxiv - Immunology 2021Quote: ... the cells were pre-incubated for 30 min at 37°C with 5 μg/mL of anti-TLR2 monoclonal antibody (clone T2.5, InvivoGen) or with an isotype control (mIgG1 ...
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bioRxiv - Immunology 2021Quote: The variable light and heavy chain sequences of KB2 and 29E3 antibodies 30,53 were cloned into cloned into pFuse vectors (pFUSE-hIgG1-Fc2 and pFUSE2ss-CLIg-hK, Invivogen). KB2 binds to the stalk domain of H1 viruses ...
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bioRxiv - Immunology 2024Quote: ... with 10 μg anti-CLEC4A4-OVA or 10μg OVA-coupled-isotype matched control antibody as described (49) plus 0.2 μg/g body weight of CpG-B ODN 1826 (InvivoGen). 12 hours later 300 cDC1 ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.
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bioRxiv - Bioengineering 2024Quote: ... incubated overnight in R10 medium with 60 IU mL-1 IL-2 and 100 µg mL-1 Normocin™ antibiotic (Invivogen, CAT#ANT-NR-1). The next day ...
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bioRxiv - Physiology 2021Quote: ... Neutralizing mouse anti-human IL-1α and human IL-1β monoclonal IgG1 antibodies and mouse IgG1 isotype control (mabg-hil1a, mabg-hil1b, mabg1-ctrlm) were purchased from InvivoGen and used at the indicated concentration with a 2 h pre-incubation in CM+ prior to addition to FAPs ...
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bioRxiv - Neuroscience 2023Quote: ... They were incubated overnight in blocking buffer solution with the primary Anti-mDectin-1-IgG antibody (1:50; cat# mabg-mdect, Invivogen) at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... 4 or 8 µg/ml) or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Immunology 2021Quote: ... BMDCs were surface-labeled with fluorochrome-coupled antibodies to the following antigens (all 1:400): MHCII-v450 (Invivogen, 48-5321-82), CD11b-PerCP-Cy5.5 (Invivogen ...
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bioRxiv - Neuroscience 2021Quote: ... or the same amount of IgG negative control antibody (Bio-Rad, MCA6004GA for MARCO and Invivogen, bgal- mab10-1 for TLR2) was administered in the same needle with IL-33.
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bioRxiv - Pathology 2020Quote: ... a truncated HSP27 peptide consisting of the C-terminal half of the peptide, (100 µg, 75 µL) mixed with an aluminum hydroxide gel adjuvant (Alhydrogel® 2%, Al, 25 µL; Invivogen vac-alu-250, San Diego, CA). One week after the commencement of E2 and/or vaccinations ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...
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bioRxiv - Microbiology 2023Quote: IgA1 heavy chains were generated by cloning the variable regions of selected antibodies into the expression vector pFUSEss-CHIg-hA1 (Cat# pfusess-hcha1, Invivogen, San Diego, CA). Variable regions of the antibody coding sequences were PCR amplified using the IgG1 heavy chain expression plasmid as a template and custom primers that appended an EcoRI site and an NheI site at the 5’ and 3’ ends respectively ...
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bioRxiv - Immunology 2023Quote: The ADCC potential of individual monoclonal antibodies was measured as described (25) using a Jurkat Lucia NFAT cell line (Invivogen, jktl-nfat-cd16), cultured according to the manufacturer’s recommendations ...