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Citations for Invivogen :
351 - 400 of 518 citations for 3 hydroxy 20 oxopregn 5 en 21 yl acetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and the protein was first adsorbed on 200 μg of aluminum hydroxide (alum; containing 100 μg of aluminum) before 20 μg of CpG1826 (vac-1826-1, Invivogen) were added at the point of injection ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 1% sorbitol and either 20 mg/liter phleomycin (after adjusting the pH to 7) or 150 mg/liter hygromycin Gold (both InvivoGen). For solid medium ...
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bioRxiv - Immunology 2022Quote: ... 50,000 T cells were co-cultured with 10,000 sorted APCs in 96-well V-bottom plates (Cellstar) in the presence or absence of 20 μg/mL OVA323-339 peptide (Invivogen). To assess cytokine production ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatant (20 μL) was then removed from each well and added to 180 μL of QUANTI-Blue detection buffer (InvivoGen). The detection solution was incubated at 37 °C for 0.25-6 h until the lowest standard had visibly turned blue ...
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Biochemistry 2022Quote: ... Female Balb/c mice (6-8 weeks old) were immunized with 15 μg of peptide adjuvanted with 20 μg CpG (ODN1826, InvivoGen) and 500 μg Alhydrogel (InvivoGen ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.8×106cells seeded per micropattern in 3mL of CM with 20 ng/ml bFGF and 100 μg/ml Normocin (InvivoGen).
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bioRxiv - Genetics 2024Quote: ... and nitrate salts are as described in the appendix of reference (Kafer E, 1977) supplemented with 1% sorbitol and 20 mg/liter phleomycin (InvivoGen) after adjusting the pH to 7.0 ...
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bioRxiv - Immunology 2024Quote: ... The macrophages were then polarized to M1 by first adding 20 ng/mL of recombinant IFNγ for 24h and then 100 ng/mL of LPS (Invivogen) for another 24h ...
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bioRxiv - Cell Biology 2022Quote: ... Infected cells were selected by incubation in medium containing 200 µg/mL hygromycin B Gold or 3 µg/mL blasticidin S (InvivoGen) for 4 d ...
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bioRxiv - Immunology 2021Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Genetics 2020Quote: ... Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen). Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR ...
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bioRxiv - Immunology 2021Quote: ... At the time of seeding 3 tumor fragments were additionally treated with either vehicle control (endotoxin-free water) or CpG ODN 2006 (InvivoGen) at 0.5ug/mL ...
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bioRxiv - Immunology 2022Quote: ... immunization with 50 µg TNP-KLH prepared with 1/3 volume of either alum or aluminum hydroxide gel (alhydrogel, Invivogen); 20 µg recombinantly produced trimer-stabilized HA (see below ...
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bioRxiv - Microbiology 2022Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Cell Biology 2024Quote: ... Infected cells were selected by incubation in medium containing 3 µg/mL blasticidin S or 200 µg/mL hygromycin B Gold (InvivoGen) for 4 d.
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bioRxiv - Immunology 2023Quote: ... the cells were pre-incubated with the respective antagonist as suggested by the manufacturer’s protocol (STING: H-151 for 1-2 hours and cGAS: RU.521 for 3 hours, both InvivoGen, USA) in cell growth medium ...
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bioRxiv - Immunology 2023Quote: ... alongside 100 μg mIgG1 anti-CD40 mAb (3/23, mouse IgG1 generated in-house as described previously[29, 30]) and the indicated doses of DMXAA(Invivogen), ADU-S100 was obtained from Oxeltis(Montpellier ...
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bioRxiv - Immunology 2023Quote: ... whole splenocytes from OT-1 mice were electroporated on day 3 after initial activation with 1 µg/mL OVA 257-264 peptide (InvivoGen), and cells were further expanded for 4 days with 200 U/mL rhIL-2 (NCI) ...
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bioRxiv - Immunology 2023Quote: ... mice were immunized with 50 μg (day 3 analysis) or 100 μg (day 7 analysis) chicken ovalbumin (OVA) protein (InvivoGen) in PBS ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 ITGB1 KO cells were transduced with pWPI-ITGB1 and selected in cDMEM supplemented with 400 μg/ml G418 (Invivogen). S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2024Quote: ... 293T and Calu-3 cells were transduced with murine leukemia virus-based transduction vectors encoding ACE2 and subsequently selected with puromycin (Invivogen), resulting in cell lines that stably expressed ACE2 ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were passaged 2-3 times a week and tested for mycoplasma contamination on routine basis using Mycostrip (Invivogen). Cells were treated with indicated concentration of oligonucleotides or Enpatoran (MedChemExpress ...
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bioRxiv - Cell Biology 2022Quote: ... followed by stimulation with 20.7 μM nigericin (Invivogen tlrl-nig; stock 5 mg/ml in ethanol) for 2 hours and 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... ABCs were induced in the presence of R848 (500 ng/ml, Invivogen, Cat#tlrl-r848-5), anti-CD40 (1 ug/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Melanocytes were further isolated by 5-day exposure to 10µg/mL G418 (InvivoGen, ant-gn-1). BRAF status was confirmed via sanger sequencing (Quintarabio ...
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bioRxiv - Molecular Biology 2021Quote: ... WT and E203Q in presence of puromycin and 200 µg/ml G418 (InvivoGen, #ant-gn-5). shRNA expression was induced by adding Doxycycline (Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec) and 5 μg/ml L-18-MDP (InvivoGen) + 10 ng/ml human interferon gamma ...
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bioRxiv - Immunology 2021Quote: ... these were co-cultured 1:1 in complete RPMI containing OVA peptide (5 mg/mL, InvivoGen) and recombinant TGFβ (1 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... at 100 ng/ml or transfected with 5 μg/ml high molecular weight (HMW) PolyIC (Invivogen) using Liopfectamine 2000 (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: ... After 18 h cells were mock-transfected or transfected with 5 μg/ml HMW PolyIC (Invivogen) using Lipofectamine 2000 (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... seeded at low density the day after transfection and selected with Ganciclovir (5 μg/mL, Invivogen) for one week ...
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bioRxiv - Immunology 2024Quote: ... A separate set of mice were challenged intraperitoneally with 5 µg LPS (serotype O55:B5; Invivogen) serum was collected after 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... The cell line was maintained as above in medium containing 5 µg/ml of blasticidin (Invivogen) and 100 µg/ml of hygromycin B (Invivogen) ...
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bioRxiv - Microbiology 2023Quote: ... 5 pmol of the IVT cbVG or Low Molecular Weight polyinosine-polycytidylic acid (poly I:C, InvivoGen) were transfected into control A549 or RNAseL-KO A549 cells ...
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bioRxiv - Immunology 2024Quote: ... monocytes were stimulated with 200 ng/mL lipopolysaccharide (LPS) and 5 mM ATP (both from InvivoGen). Monocyte supernatants were collected 5 hours post-stimulation and cytokine production was measured by LEGENDPlex™ using a 10-plex Human M1/M2 Macrophage Panel.
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Microbiology 2022Quote: ... RIG-I stimulation was assessed by analyzing Lucia luciferase reporter gene expression in 20 µl cell culture supernatants using QUANTI-Luc™ (Invivogen). Data are expressed as the normalized fold change over mock infected cells ...
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bioRxiv - Systems Biology 2021Quote: ... 20 μL of the cell media supernatant was added to 50 μL of QUANTI-Luc™ assay solution (Invivogen #rep-qlc1) in a white 96-well microplate (Greiner ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of supernatants were mixed with 180 μl of the working solution of the QUANTI-Blue reagent (InvivoGen #rep-qbs) and incubated at 37 °C for 0.1-3 h ...
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bioRxiv - Microbiology 2024Quote: ... and 20 µL of supernatant was added to 180 µL of QUANTI-Blue Alkaline Phosphatase Detection Medium (InvivoGen catalog # rep-qbs), incubated at 37°C with shaking ...
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bioRxiv - Immunology 2024Quote: ... Plates were then centrifuged for 5 min at 500 g and 80 µL of the supernatant was added to a different opaque 96-well plates containing 20 µL of QUANTI-Luc™ Luciferase Detection Reagent (InvivoGen). With the lid off ...
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bioRxiv - Cell Biology 2024Quote: ... followed by two rounds of selection alternating between BCDA medium containing the appropriate antibiotic (30 µg/mL hygromycin B [Carl Roth], 20 µg/mL G418 [Merck], 50 µg/mL zeocin [Thermo Fisher Scientific], or 75 µg/mL blasticidin [InvivoGen]) according to the transformed resistance cassette and BCDA medium (supplemented with 100 µg/ml vancomycin) ...
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bioRxiv - Cancer Biology 2023Quote: ... Regular testing for mycoplasma infection was conducted every 3–4 months using the PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Immunology 2020Quote: ... mice were immunized with an initial subcutaneous injection of 200 μg antigen (and 20 μg monophosphoryl lipid A (MPLA, Invivogen, Tlrl-mpla) adjuvant ...
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bioRxiv - Immunology 2022Quote: ... indicated dose of conjugated or free SOSIP proteins (25 μg mi3-conjugated SOSIP per mouse in most experiments) were mixed with 20 μg MPLA (Invivogen, vac-mpls) and 10 μg Quil-A (Invivogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Bioengineering 2024Quote: ... 0.125 μg each variant) per dose in either bolus (PBS) or in PNP hydrogels with or without 20 μg per dose MPLAs (Invivogen, vac-mpls) or 1 μg per dose 3M-052 (AAHI) ...