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Citations for Invivogen :
351 - 400 of 453 citations for 2 AMINO 3 METHYLTHIOMETHYL BENZOTRIFLUORIDE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... the media was replaced with fresh culture media containing 2 µg/mL puromycin (InvivoGen, ant-pr-1). After approximately 1.5 days of selection ...
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bioRxiv - Immunology 2021Quote: ... Mice (8 mice per group) were intramuscularly injected twice at four-week intervals with each VLPs (HA content=3 µg) with AddaVax™ adjuvant (Invivogen, San Diego, CA, USA) (Figure 2) ...
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bioRxiv - Immunology 2022Quote: ... MDDCs were harvested and cultured over-night in media alone or in the presence of 5 μg/ml of a pool of 15-mer overlapping HIV-1 Gag peptides provided by the NIH AIDS Reagent Program (#11057) either individually or in combination with 1 μg/ml of 2’3’-c’diAM(PS)2 (Invivogen) STING agonist and 2.5 μg/ml of Poly I:C (SIGMA ...
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bioRxiv - Immunology 2021Quote: ... in a final volume of 100 µL X-VIVOTM-15 + 2% HS + 1 µg.mL-1 poly(I:C) (tlrl-picw, Invivogen). After 3 h ...
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bioRxiv - Immunology 2021Quote: ... Specific wells were pre-cultured for 2 hours with either 10mg/ml of TLR4 inhibitor (CLI-095, Invivogen) or 10mg/ml PI3 Kinase inhibitor (LY294002 ...
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bioRxiv - Microbiology 2020Quote: ... except that the protoplasts were transformed with 1μg of each split-marker cassette DNA and plated on medium containing 200 g.l−1 saccharose and 2 g.l−1 NaNO3 supplemented with 70 μg.ml−1 hygromycin (Invivogen, France) for single ΔBcflp1 or ΔBcflp2 mutants or 80 μg.ml−1 nourseothricin (Werner BioAgents ...
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bioRxiv - Bioengineering 2022Quote: ... 293-cov2-sdf) or 2) Delta/B.1.617.2 variant) (Catalogue code: 293-SARS2-S-V8-dfur) were purchased from Invivogen. The cells were grown in DMEM media containing 10% Fetal Bovine Serum ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed and cultured for 5-7 days in the presence of puromycin (2 µg/ml, InvivoGen). Selected cells were expanded ...
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bioRxiv - Immunology 2021Quote: ... injection of 10 mg/kg poly(I:C) for 6h followed by 2 mg/kg ultrapure LPS-B5 (InvivoGen) prepared from E ...
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
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bioRxiv - Genomics 2023Quote: ... cells were cultured in the same medium supplemented with 200 μg/ml HygromycinGold B (Invivogen ant-hg-2). To generate cells with constitutive BFP expression ...
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bioRxiv - Bioengineering 2023Quote: ... The transfected cells were expanded to T-75 flasks and cultured with 2 µg/mL of puromycin (Invivogen), 10 µg/mL of blasticidin (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stimulated by adding 40 ml of fresh medium containing 1µg/ml of 2’3’-cGAM(PS)2 (Invivogen) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... the cultures were incubated with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Microbiology 2023Quote: ... ACE2 and transmembrane serine protease 2 (TMPRSS2)-expressing A549 (ACE2-TMPRSS2-A549) cells were from Invivogen (#a549-hace2tpsa) and HEK293T were from ATCC (#CRL-11268) ...
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bioRxiv - Biochemistry 2020Quote: ... parasites were cultured with anhydrotetracycline medium at 250 nM (aTc+, MilliporeSigma) for 2 days before blasticidin /aTc+ medium was used (blasticidin at 2.5 µg/mL, InvivoGen).
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Immunology 2021Quote: ... in the left and right mid-thighs with 50 µg MD39 and 500 µg alum (Alhydrogel adjuvant 2%; InvivoGen) per side ...
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bioRxiv - Neuroscience 2021Quote: ... endotoxin levels in exosomes and iPS-Mg medium were measured using the HEK Blue LPS detection kit 2 (InvivoGen) following manufacturers’ instructions ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... experiments were grown in Dulbecco’s modified Eagle’s medium DMEM supplemented with 10% fetal bovine serum and 50ug/ml normocin (ant-nr-2, Invivogen). HEK293 cells and other cell lines used were grown at 37°C and 5% CO2.
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bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-coated with laminin for 24 hr prior to treatment with MCC950 (1 μM, 2 hr, Invivogen, inh-mcc) followed by 15 min exposure to isoproterenol (100 nM) ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured for 2 weeks in the presence of 1 µg/mL puromycin (ant-pr-1, InvivoGen). The cells were single-cell-cloned using a limiting dilution method ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2022Quote: 2 × 106 pleura cells were plated and stimulated with 1 µg/mL poly(I:C) (Invivogen, San Diego, CA, USA) for 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... or RPMI1640 (ATCC) supplemented with 10% FBS and 2 mM L-Gln (MC38) and 4 µg/mL Blasticidin (InvivoGen) (MC38-Her2-B2m-/-) ...
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bioRxiv - Immunology 2023Quote: ... of filtered PAO1 supernatant or TSB and KGMTM-2 containing purified flagellin at 1μg/ml or vehicle (tlrl-pafla; from P. aeruginosa; InvivoGen) were used for treating hTCEpi cells for various durations as indicated in the figure legends.
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bioRxiv - Immunology 2022Quote: ... CASP8-/- × MLKL-/- and subsequently derived gene-deficient BLaER1 macrophages were stimulated with 2 ng/mL LPS from E.coli (Invivogen) for 18 h ...
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bioRxiv - Genomics 2022Quote: ... After 2 to 6 minutes of gentle manual shaking in ice cold PBS with 1% (v/v) primocin (InvivoGen), the intestinal pieces were inspected microscopically (x100 magnification ...
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bioRxiv - Molecular Biology 2024Quote: ... and stable donor cell lines expressing MCP-CD63 were cultured in the presence of 2 μg/ml puromycin (Invivogen).
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) Primocin™ (InvivoGen, San Diego, CA; ant-pm-1) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Microbiology 2020Quote: ... A total of five adjuvants were tested for their efficacy within the vaccine formulations: Aluminum hydroxide gel (alum) (Alhydrogel adjuvant 2%, InvivoGen), Polyinosinic-polycytidylic acid (poly (I:C ...
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bioRxiv - Cancer Biology 2021Quote: We transduced cells with lentivirus carrying KRAB-dCas9 and sgRNA targeting candidate progrowth enhancers and performed puromycin (2 μg/ml) (InvivoGen) for three days postelectroporation to select against non-transduced cells ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... In some cases, cells were first pre-treated for 1 h with following inhibitors (2 µM H-151, 10 µM MCC950 (InvivoGen)) before addition of the treatments ...