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Citations for Invivogen :
351 - 400 of 1365 citations for 1 3 Bis 2 4 hydroxyphenyl 2 propyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: We transduced cells with lentivirus carrying KRAB-dCas9 and sgRNA targeting candidate progrowth enhancers and performed puromycin (2 μg/ml) (InvivoGen) for three days postelectroporation to select against non-transduced cells ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Genomics 2024Quote: ... A total of 17,114 BFP+ cells were replated into a half deep 96 well plate (Applikon Biotechnology) and allowed to expand with the addition of 2 μg/mL doxycycline and 100 μg/mL blasticidin (Invivogen) to select for functional landing pad cells ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed bone marrow cells were cultured in RPMI supplemented with 10% FBS with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Immunology 2023Quote: ... followed by the medium being replaced with fresh IFNγ (5 ng/ml) containing media for 2 h before treatment with indicated stimulations: MDP (100 μM, Invivogen), iE-DAP (100 μM ...
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bioRxiv - Genetics 2023Quote: ... 5% CO 2 prior to stimulation under the same conditions with the following reagents and concentrations - Pam3Csk4 (Invivogen, Lot no.), LPS (Invivogen ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were expanded to a surface-treated T-75 flask and treated with 2 µg/mL of puromycin (Invivogen) for 2 weeks for selection of the transduced cells to generate the cell line expressing hM3D(Gq) ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Cell Biology 2024Quote: The transfected MDCK cells were selected using 400 μg/ml G418 disulfate (#08973-14, NacalaiTesque) or 200 μg/ml hygromycin B Gold (#ant-hg-2, Invivogen), the mixed clones with the lowest EGFP expression were collected by a FACS (SH800Z ...
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bioRxiv - Genomics 2022Quote: ... and incubated in fresh 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen) for 40 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Transduced cells were selected with 3 μg/mL puromycin (InvivoGen, San Diego, CA, catalogue #ant-pr-1) for 3 days ...
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bioRxiv - Biophysics 2024Quote: ... 3 μg/ml puromycin (InvivoGen), was exchanged for PBS buffer (DPBS ...
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bioRxiv - Cell Biology 2024Quote: ... diABZI compound 3 (Invivogen, 1µM).
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bioRxiv - Immunology 2024Quote: ... with diABZI compound 3 (InvivoGen) at 10 µM ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... where n=2) were immunized with the indicated immunogen in 100 μL of 50% v/v of AddaVax™ adjuvant (Invivogen) and boosted with adjuvant on Day 14 ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... co-transfected cells were selected in culture medium supplemented with 3 μg/ml puromycin (Invivogen, ant-pr-1), until selection was complete after about 48 h ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 24 h post infection in 4 µg/ml Blasticidin (Invivogen, ant-bl-1) or 100 µg/ml of hygromycin (Invivogen ...
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bioRxiv - Immunology 2024Quote: ... followed by priming for 4 hours with 1 μg/mL lipopolysaccharides (LPS, Invivogen, Cat. No: tlrl-b5lps) before stimulation with 20 μM nigericin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... and puromycin (3 ug/mL) (InvivoGen). All cell lines were grown at 37°C in 5% CO2.
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bioRxiv - Immunology 2022Quote: ... and puromycin (3 μg/mL) (InvivoGen). IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µg/ml Blasticidin S (InvivoGen) and 0.5 µg/ml Puromycin (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and 3 μg/mL puromycin (Invivogen). For immunoblotting experiments ...
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bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
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bioRxiv - Microbiology 2020Quote: ... DNA ratio of 3:1 according to manufacturer’s instructions or with Poly(I:C) (LMW)/LyoVec™ (tlrl-picwlv, InvivoGen). The treatment was administered as indicated ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Immunology 2020Quote: Undifferentiated THP-1 and PMA-differentiated cells (2 × 105 cells) in biological duplicates per condition were stimulated with TLR agonists- CL075 (TLR8; tlrl-c75; Invivogen, 5 μg/ml), CpG2006 (TLR9 ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Microbiology 2024Quote: ... low-passage vials of cells were thawed and incubated in Corning Falcon 75 cm2 vented culture flasks at a density of 2 x 106 / 15 mL of DMEM with 100 µg/mL Normocin (InvivoGen catalog # ant-nr) for approximately 72 hours ...
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bioRxiv - Biochemistry 2024Quote: Viral dsRNA-induced infection in cells was mimicked by treating the cells with 2 µg/mL of poly(I:C) LMW (InvivoGen, Cat. Code: tlrl-picw) dissolved in sterile PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Transduced cells were selected by supplementing DMEM culture media with 4 µg/ml puromycin (Invivogen, ant-pr-1). In RKO-Cas9 cells genome editing was induced using 200 ng/ml or 350 ng/ml final concentration of doxycycline hyclate (Dox ...
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bioRxiv - Molecular Biology 2021Quote: ... + 4 μg/ml Blasticidin (Invivogen). In order to knock-out SLX4 gene in these cells ...