Labshake search
Citations for Invivogen :
301 - 350 of 487 citations for 20 Hydroxyecdysone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... mice were immunized with an initial subcutaneous injection of 200 μg antigen (and 20 μg monophosphoryl lipid A (MPLA, Invivogen, Tlrl-mpla) adjuvant ...
-
bioRxiv - Immunology 2022Quote: ... indicated dose of conjugated or free SOSIP proteins (25 μg mi3-conjugated SOSIP per mouse in most experiments) were mixed with 20 μg MPLA (Invivogen, vac-mpls) and 10 μg Quil-A (Invivogen ...
-
bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
-
bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... Cells were washed the next day and subjected to puromycin selection (2µg/ml, Invivogen ant-pr-5) 48hrs later ...
-
Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were then selected with Blasticidin at 5 μg/mL and Puromycin (1 μg/mL, Invivogen, USA) to select for cells stably expressing both dCas9 and the sgRNAs ...
-
bioRxiv - Cell Biology 2020Quote: ... mice were serially injected intraperitoneally (i.p.) with 5 mg/kg high molecular weight polyinosinic:polycytidylic acid (pI:pC, InvivoGen), which had been reconstituted in sterile physiologic saline precisely as described in the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Labeled cells (0.5x106 cells/mL) were cultured for 2-3 days with 5 μg/mL R848 (InvivoGen), 50 U/mL IL-2 (Peprotech) ...
-
bioRxiv - Immunology 2023Quote: ... compounds were added 2 hr before stimulation of cells at the following concentrations: 5 µM MCC950 (Invivogen), 10 µg/mL Ac-YVAD-cmk (Invivogen) ...
-
bioRxiv - Microbiology 2023Quote: ... cells were treated with 5 μg/mL puromycin (InvivoGen, San Diego, CA, USA, Cat# ant-pr-1). After 1 week of incubation ...
-
bioRxiv - Cell Biology 2024Quote: ... Flip-In T-Rex cells were maintained using hygromycin B (30 µg/mL, InvivoGen, ant-hg-5) and blasticidin (30 µg/mL ...
-
bioRxiv - Immunology 2023Quote: ... Serum flow-through was then passed through 1 mL of Peptide M agarose (InvivoGen, gel-pdm-5) affinity column in gravity mode ...
-
bioRxiv - Immunology 2024Quote: ... done in duplicate wells for each condition) were stimulated with 5 uM CpG-A (ODN 1585, Invivogen) or CpG-C (ODN 2395 ...
-
bioRxiv - Immunology 2022Quote: ... we read the plate on Cytation 7 (Cytation 7, Bio-Tek Instruments, Inc.) after adding 50 μL QUANTI-Luc™ (Invivogen) substrate solution per well ...
-
bioRxiv - Microbiology 2021Quote: ... 50 µL of each cell supernatant were transferred into separate plates and added 50 µL of quantiblue reagent™ following manufacturer’s instructions (InvivoGen Inc.). Afterwards ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Strains containing the pHK-Cre-EBDh were selected on YDP agar plates supplemented with 200 µg/mL hygromycin B (Invivogen, USA).
-
bioRxiv - Immunology 2022Quote: ... we read the plate on Cytation 7 (Cytation 7, Bio-Tek Instruments, Inc.) after adding 50 μL QUANTI-Luc™ (InvivoGen) substrate solution per well ...
-
bioRxiv - Microbiology 2022Quote: ... 20 µl of cell supernatant were transferred to new plates and supplemented with freshly made 180 µl Quanti-Blue solution (Invivogen, France). After 180 minutes of incubation ...
-
bioRxiv - Immunology 2023Quote: ... the cells were centrifuged and 25 µL of supernatant was drawn from each well and transferred into 96-well white walled clear bottom polystyrene plate (Costar) and mixed with 75 µL of reconstituted QUANTI-Luc™ reagent (InvivoGen). Luminescence was immediately read on a SpectraMax Paradigm Plate reader (Molecular Devices ...
-
bioRxiv - Bioengineering 2023Quote: ... 20 µL of culture media was transferred to a 96-well clear-bottom black plate and 50 µL of QUANTI-Luc™ assay solution (Catalog Code: repqlc; Invivogen) was added directly to each well ...
-
bioRxiv - Immunology 2021Quote: ... determined using the Endotoxin Detection kit (InvivoGen).
-
bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
-
bioRxiv - Microbiology 2022Quote: ... About 6-8-week-old C57BL/6 mice were immunized with 20 µg (50 µl) purified antigen in PBS using Addavax (Invivogen, San Diego, CA) as the adjuvant ...
-
bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...
-
bioRxiv - Immunology 2023Quote: ... NF-κB activation was determined after 20 h of incubation according to the manufacturer’s instructions by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen, San Diego, CA, USA). The plates were incubated for 2 hours at 37°C ...
-
bioRxiv - Cancer Biology 2023Quote: ... NF-kB activity was measured by combining 20 μL of supernatant with180 μL QUANTI-Blue SEAP detection reagent (InvivoGen, cat no. rep-qbs) in 96-well assay plate ...
-
bioRxiv - Immunology 2024Quote: ... low-endotoxin nanoparticles were diluted to 20 μg/mL in buffer and mixed with 1:1 v/v AddaVax adjuvant (InvivoGen vac-adx-10) to obtain a final dose of 1 μg of immunogen per animal ...
-
bioRxiv - Genomics 2019Quote: ... supplemented with 5% human platelet lysate (Cook Regentec, #G34936) and 50 µg/mL Primocin (InvivoGen, #ant-pm-1). hASCs were cultured in the same media composition until 70-80% confluency and detached using TrypLE Select (Life Technology ...
-
bioRxiv - Bioengineering 2021Quote: The vaccines contained a 10 µg dose of RBD and combinations of 5 µg Quil-A Adjuvant (Invivogen), 50 µg Resiquimod (R848 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed and cultured for 5-7 days in the presence of puromycin (2 µg/ml, InvivoGen). Selected cells were expanded ...
-
bioRxiv - Cancer Biology 2021Quote: ... the medium was exchanged for 10 mL DMSO-free medium containing 5 mg/mL Primocin (Invivogen, Toulouse, France), the tissue was minced in 10mL basal NSC in a cell culture petri dish using a sterile scalpel blade ...
-
bioRxiv - Immunology 2020Quote: Resiquimod gill challenge was performed as previously described (33) using 5 μL of Resiquimod (0.5 mg/mL; Invivogen) applied to the gills for 5 min ...
-
bioRxiv - Immunology 2019Quote: ... Stained cells were cultured for 5 days in BCM supplemented or not with CpG (ODN2006, 2,5µg/ml, Invivogen), or cocultured with MS40 cells expressing CD40L and cytokine cocktail (recombinant human BAFF (10 ng/ml) ...
-
bioRxiv - Microbiology 2020Quote: ... All HEK293 Flp-In T-REx cell lines were additionally supplemented with 5 µg/mL Blasticidin S (Invivogen) and 200 µg/mL Hygromycin B (Invivogen ...
-
bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
-
bioRxiv - Bioengineering 2022Quote: ... CD8+ T cells and 5×104 NALM-6 cells were plated with 0.5-1 ng/mL blinatumomab (Invivogen) and cytokine ...
-
bioRxiv - Cell Biology 2023Quote: ... the cells were selected using McCoy’s medium containing 2.5 – 5 µg/mL Blasticidin S (Invivogen # ant-bl-05) for 7 days ...
-
bioRxiv - Genetics 2024Quote: ... 5 µL of the ligated construct were transferred to chemically competent E.coli GT115 cells (pir mutant strain, Invivogen) and spread on lysogeny broth (LB ...
-
bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
-
bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 12-well plate in 1 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr) or 200 μg/mL hygromycin (Millipore ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were allowed to recover overnight at room temperature without shaking and then plated on YPD agar plates supplemented with 200ug/mL G418 (Invivogen; ant-gn). Plates were incubated at 30C until colonies formed ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were allowed to recover overnight at room temperature without shaking and then plated on YPD agar plates supplemented with 200ug/mL G418 and 200ug/mL Zeocin (Invivogen; ant-zn). Plates were incubated at 30C until colonies formed.
-
bioRxiv - Cancer Biology 2021Quote: ... examined for mycoplasma regularly (detection kit from Invivogen). A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962 ...
-
bioRxiv - Cancer Biology 2021Quote: ... To analyze the interferon response HEK-Dual hTLR3 cells were treated with 5 μg/ml poly(I:C) HMW (InvivoGen) or the different extracted media for four hours ...
-
bioRxiv - Immunology 2019Quote: ... or PAL-CRID3 (0.001-100 µM) for 30 min and then stimulated with 5 μg mL−1 nigericin (Invivogen) for 30 min.
-
bioRxiv - Microbiology 2021Quote: ... 5 μg of SpK combined with either BCG (BCGSpK) or 100 μg of Alhydrogel (Alum) (Invivogen, California, USA, AlumSpK), or a combination of BCG (5×105 CFU) ...
-
bioRxiv - Microbiology 2020Quote: ... using the calcium phosphate precipitation technique and selected by treating the cells with 5 µg/mL Blasticidin S (Invivogen) and 200 µg/mL Hygromycin B (Invivogen).