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Citations for Invivogen :
251 - 288 of 288 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Neuroscience 2021Quote: ... SEAP activity in the culture supernatants were measured with QUANTI-Blue™ Solution (Invivogen) following the manufacture’s protocolThe data were analyzed in comparison with control empty vector transfection after SEAP normalization ...
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bioRxiv - Microbiology 2024Quote: ... 150 µL of 15 mg/mL of isolated peptidoglycan solution in LAL water (Invivogen) was mixed 1:1 with 5% sucrose and fed to flies from filter discs to test the expression of Dpt in the gut upon PNG ingestion ...
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bioRxiv - Bioengineering 2022Quote: ... The injector was primed with QUANTI-Luc assay solution (Invivogen, cat. code rep-qlc1), and the measurement was taken ...
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bioRxiv - Neuroscience 2021Quote: ... resiquimod (2 mg/kg of a 1 mg/ ml solution in PBS, Invivogen tlrl-r848), were administered subcutaneously on E12.5 between 9:00-11:00 am and their conditions were monitored to ensure that there was no sign of any severe sickness symptoms or abnormalities.
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bioRxiv - Biochemistry 2023Quote: ... and 50 µL of the working solution of the QUANTI1Luc 4 Lucia/Gaussia reagent (Invivogen) was added ...
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bioRxiv - Immunology 2024Quote: ... 30ul of the cell supernatant was incubated with 170ul Quanti-Blue solution (Invivogen, rep-qbs) and incubated for 1h at 37°C ...
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bioRxiv - Genetics 2024Quote: ... and 30 µl was incubated with 170 µl of QUANTI-blue solution (Invivogen, #rep-qbs) in a new 96-well plate ...
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bioRxiv - Microbiology 2023Quote: ... The relative light unit (RLU) was detected by Quanti-Luc substrate solution (25 µL/well, Invivogen) using a Polerstar optima microplate reader (BMG BioLabtech) ...
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bioRxiv - Immunology 2023Quote: ... and absorbance levels detected at 655 nm after 3 h incubation with QUANTI-Blue Solution (Invivogen). The absorbances of RAW-Blue assay were normalized to absorbance intensity at the highest and lowest dilutions ...
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bioRxiv - Immunology 2024Quote: ... we incubate THP1-Blue cells with 180 μL of QUANTI-Blue™ Solution (Invivogen, rep-qbs) and 20 μL of supernatant containing different stimuli as described above in a 96-well plate ...
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bioRxiv - Neuroscience 2021Quote: ... poly I:C (20 mg/kg of a 10 mg/ml solution in PBS, LMW, Invivogen tlrl-picw), resiquimod (2 mg/kg of a 1 mg/ ml solution in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 μL of the conditioned medium was mixed with 180 μL QUANTI-Blue solution (InvivoGen, rep-qbs) and incubated for 2-4 h at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... and absorbance levels were detected at 655 nm after 2 h incubation with QUANTI-Blue Solution (Invivogen). Nonlinear regression fits were estimated using the “Log(agonist ...
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bioRxiv - Immunology 2021Quote: ... then 20 µL media from each well was added to 180 μL room temperature QUANTI-Blue Solution (InvivoGen) in a separate flat-bottom 96-well plate and incubated at 37°C for 3 hours ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... 10 μL supernatants were collected from each well and added to 90 μL QUANTI-Blue substrate solution (InvivoGen) to detect expression of secreted embryonic alkaline phosphatase (SEAP ...
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bioRxiv - Immunology 2022Quote: ... Recipient mice were immunized intra-peritoneally (i.p.) with a 200 μl solution composed of 1 part of Alum (Invivogen, Alhydrogel® adjuvant 2% ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
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bioRxiv - Physiology 2023Quote: ... 20µl of supernatant were transferred to a fresh 96-well plate and incubated with 180µl of QUANTI-Blue solution (Invivogen) at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of conditioned media was aliquoted and mixed with Quantiblue solution (rep-qbs2; Invivogen, San Diego, CA, USA) and incubated in a 96-well plate for an additional 4 hours before quantification via plate reader.
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bioRxiv - Bioengineering 2024Quote: ... The alum solution was made by matching the amount of PEG by mass to the aluminum in Alhydrogel (alum, InvivoGen), resulting in a solution of 36% alum by volume in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... They were incubated overnight in blocking buffer solution with the primary Anti-mDectin-1-IgG antibody (1:50; cat# mabg-mdect, Invivogen) at 4°C ...
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bioRxiv - Genetics 2022Quote: ... 20 uL of supernatant from each well was collected and mixed with 50 uL of Quanti-Luc Gold solution (rep-qlcg1, Invivogen) in a white 96-well microplate (655075 ...
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bioRxiv - Microbiology 2023Quote: ... The secreted embryonic alkaline phosphatase (SEAP) was measured in a microplate reader (OD Ex/Em = 620/655 nm) using Quanti-Blue Solution (InvivoGen), following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... were transferred to a new 96-well plate (Costar, 3370) and mixed with 180 μl of pre-warmed Quanti-Blue Solution (InvivoGen). After 1 h of incubation in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... 90 µL of the supernatant was collected from each well and mixed with 90 µL of QUANTI-Blue solution (rep-qbs2, InvivoGen). The mixture was incubated at 37 °C for 15–30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µL of cell supernatant was transferred to a 96-well plate containing 180 µL of QUANTI-Blue assay solution (InvivoGen) to measure SEAP levels ...
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bioRxiv - Systems Biology 2021Quote: ... 20 μL of the cell media supernatant was added to 50 μL of QUANTI-Luc™ assay solution (Invivogen #rep-qlc1) in a white 96-well microplate (Greiner ...
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bioRxiv - Microbiology 2022Quote: ... Subcutaneous vaccination was performed by inoculation with 100 µL of vaccine solution containing 10 µg of ODN2006 (InvivoGen, San Diego, CA) or 1 mg of Imject Alum adjuvant (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP) activity into cells supernatant using QUANTI-Blue™ Solution (Invivogen), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl of supernatants were mixed with 180 μl of the working solution of the QUANTI-Blue reagent (InvivoGen #rep-qbs) and incubated at 37 °C for 0.1-3 h ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl of cell supernatant were transferred to new plates and supplemented with freshly made 180 µl Quanti-Blue solution (Invivogen, France). After 180 minutes of incubation ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of culture media was transferred to a 96-well clear-bottom black plate and 50 µL of QUANTI-Luc™ assay solution (Catalog Code: repqlc; Invivogen) was added directly to each well ...
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bioRxiv - Immunology 2024Quote: ... the supernatant was discarded and replaced with either 23 μL of control solution (HEPES and water) alone or OL or LPS diluted in the control solution and 77 μL of HEK-Blue™ Detection medium (InvivoGen) (cell culture medium for real-time detection of secreted alkaline phosphatase ...
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bioRxiv - Immunology 2023Quote: ... HEK293-C34 cells were gifted by Y Matsuura at Osaka University and maintained in high-glucose DMEM (Nacalai Tesque) containing 10% FBS and 10 μg/mL blasticidin (solution) (InvivoGen, California, USA), and the exogenous expression of ACE2 and TMPRSS2 was induced by the addition of doxycycline hydrochloride (1 μg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... by assessing the activity of a secreted Lucia luciferase using QUANTI-Blue™ Solution and QUANTI-Luc™ 4 Lucia/Gaussia (InvivoGen), respectively ...
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bioRxiv - Microbiology 2024Quote: ... Stimulation of the IFNL triggered the JAK/STAT/ISGF3 pathway and induced SEAP production which was measured by a colorimetric assay at 650 nm using Quanti-BlueTM solution according to the manufacturer’s instructions (InvivoGen, San Diego, CA, USA).
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bioRxiv - Immunology 2020Quote: ... Levels of SEAP were detected in the culture media after 3 hours incubation of supernatants with Quanti-Blue solution (InvivoGen, San Diego, CA, USA) at 650nm wavelength by ELISA reader.