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Citations for Invivogen :
151 - 200 of 291 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the cells were pre-incubated with the respective antagonist as suggested by the manufacturer’s protocol (STING: H-151 for 1-2 hours and cGAS: RU.521 for 3 hours, both InvivoGen, USA) in cell growth medium ...
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bioRxiv - Immunology 2023Quote: ... whole splenocytes from OT-1 mice were electroporated on day 3 after initial activation with 1 µg/mL OVA 257-264 peptide (InvivoGen), and cells were further expanded for 4 days with 200 U/mL rhIL-2 (NCI) ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 ITGB1 KO cells were transduced with pWPI-ITGB1 and selected in cDMEM supplemented with 400 μg/ml G418 (Invivogen). S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Immunology 2023Quote: Immune responses were induced in 6-12-week-old male and female mice by subcutaneous immunization in the right FP with 5 μg (for HA experiments) or 10 μg (for hapten-carrier experiments) supplemented with 1/3 volume alhydrogel adjuvant (Invivogen). In S1pr2-Tomato mice ...
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bioRxiv - Cell Biology 2024Quote: ... Infected cells were selected by incubation in medium containing 3 µg/mL blasticidin S or 200 µg/mL hygromycin B Gold (InvivoGen) for 4 d.
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bioRxiv - Immunology 2020Quote: ... Mouse bone marrow-derived macrophages (BMDMs) treated with 0.5 μg/ml LPS (tlrl-smlps, Invivogen) for 3 h followed by 5 mM ATP (10519987001 ...
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bioRxiv - Immunology 2022Quote: ... The protein antigen was adjuvanted with AddaVax (1:1 v/v; vac-adx-10, InvivoGen, USA) for immunisation.
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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bioRxiv - Immunology 2022Quote: ... protein samples (0.1 mg/mL) were pre-mixed with alum (10 mg/mL, Alhydrogel®, InvivoGen) at a ratio of 1:10 (protein ...
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bioRxiv - Immunology 2021Quote: ... K18-hACE2 mice of both sexes were primed and boosted at 3 weeks intervals with S (10 µg/dose) or RBD (20 µg/dose) emulsified with AddaS03™ (InvivoGen) via intramuscular route ...
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bioRxiv - Immunology 2019Quote: ... we administered plasmid DNA adjuvanted with 20 μg/mouse monophosphoryl lipid A from Salmonella minnesota (Invivogen) via the i.m ...
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bioRxiv - Immunology 2020Quote: ... WGP-S and WGP-D (500 μg/mouse for in vivo experiments) were purchased from Invivogen; diphtheria toxin (unnicked ...
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bioRxiv - Molecular Biology 2023Quote: ... partial mouse Azin1 expression plasmids were transfected using Lipofectamine 2000 in the presence of NATETM (InvivoGen) in some experiments.
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Bioengineering 2021Quote: ... Purified proteins were tested for endotoxin via HEK-Blue TLR4 reporter cell line (Invivogen, San Diego, CA) and endotoxin levels were confirmed to be less than 0.01 EU/mL ...
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bioRxiv - Immunology 2021Quote: The capacity of E protein to activate NF-kB was tested by using HEK-TLR2-blue (InvivoGen). In this assay ...
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bioRxiv - Immunology 2020Quote: ... RBD or OVA proteins were formulated in PBS at a 1:1 ratio with Addavax adjuvant (InvivoGen). C57BL/6 or BALB/c mice were anesthetised by isoflurane inhalation prior to intramuscular injection of 50 μL vaccine in each hind quadriceps ...
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bioRxiv - Bioengineering 2021Quote: ... The purified proteins were tested for endotoxin using a HEK-Blue™ TLR4 reporter cell line (InvivoGen), and the endotoxin levels were confirmed to be below 0.01 EU mL−1 ...
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bioRxiv - Immunology 2021Quote: ... determined using the Endotoxin Detection kit (InvivoGen).
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bioRxiv - Cell Biology 2022Quote: ... the cationic lipid-based transfection reagent LyoVec and cyclic [G(2’,5’)pA(3’,5’)p] (2’3’-cGAMP) were obtained from Invivogen (San Diego, USA) and Lipofectamine2000 was obtained from ThermoFisher Scientific (Dreieich ...
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bioRxiv - Bioengineering 2021Quote: ... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (ant-pm-1, InvivoGen, San Diego, California, USA). Cultures were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... for 3 hr followed by (flagellin isolated from P. aeruginosa, 2 or 5 µg/mL) for 3 hr using FLA-PA Ultrapure (InvivoGen, tlrl-pafla) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were treated with 400 µM FFA and 3 µM HSG4112 in the presence or absence of bafilomycin A1 (Invivogen, tlrl-baf1). Cellular localization of LC3B was observed using a Carl Zeiss Confocal LSM710 Meta microscope and the images were processed with the software supplied by the manufacturer (Carl Zeiss ...
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bioRxiv - Immunology 2020Quote: ... with penicillin-streptomycin (100 units/mL) and activated using 3 µM CpG (ODN 7909) (tlrl-2006-1, Invivogen, San Diego, CA, USA). Cells were cultured in 200 µl medium for 1-8 days using round bottom 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... Eluted fractions were analyzed by western blotting with a mouse monoclonal RBD-specific antibody (InvivoGen, Cat# srbdmab10). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen, San Diego, CA) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... examined for mycoplasma regularly (detection kit from Invivogen). A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962 ...
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bioRxiv - Microbiology 2021Quote: ... Eluted fractions were analyzed by western blotting with a mouse monoclonal RBD specific antibody (InvivoGen, Cat# srbd-mab10). Peak fractions were then pooled and dialyzed against PBS in 10,000 molecular weight cutoff (MWCO ...
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bioRxiv - Physiology 2023Quote: Mouse FGL1 cDNA sequences (full length, N-terminal domain, globular domain) were cloned into pFUSEN-hG2Fc plasmid (InvivoGen) with the following modifications ...
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bioRxiv - Immunology 2020Quote: ... Levels of SEAP were detected in the culture media after 3 hours incubation of supernatants with Quanti-Blue solution (InvivoGen, San Diego, CA, USA) at 650nm wavelength by ELISA reader.
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bioRxiv - Genetics 2023Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml Hygromycin (HygroGold™ InvivoGen, San Diego, CA) or Hygromycin B ...
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bioRxiv - Microbiology 2021Quote: ... the mouse received a final boost intraperitoneally with 100 μg of recombinant N6 protein of A/duck/Zhejiang/D9/2013 (H4N6) adjuvanted with 10 μg of poly (I:C) (Invivogen). Three days after the final boost ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Biochemistry 2019Quote: ... coli NucC bound to 5’-pApA or cAAA in hanging drop format by mixing protein (8-10 mg/mL) in crystallization buffer plus 0.1 mM 5’-pApA (Invivogen) or cAAA 1:1 with well solution containing 17-24% PEG 3350 ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Immunology 2020Quote: ... right and left axillary LNs) were evaluated 14 days-post IM vaccination with PR8-HA (5μg) protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink ...
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bioRxiv - Synthetic Biology 2022Quote: ... Purified protein groups formulated in PBS were mixed with an equal volume of Adju-Phos aluminum phosphate adjuvant (InvivoGen) before injection ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell cultures were routinely tested for mycoplasma contamination using Universal Mycoplasma Detection Kit (ATCC, #30-1012K) and PlasmoTest Mycoplasma Detection Kit (Invivogen, #rep-pt1).
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bioRxiv - Immunology 2021Quote: ... Mice (8 mice per group) were intramuscularly injected twice at four-week intervals with each VLPs (HA content=3 µg) with AddaVax™ adjuvant (Invivogen, San Diego, CA, USA) (Figure 2) ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... total splenocytes isolated from OT-II mouse spleens were cultured with OVA 323-339 peptide (1 mg/ml, InvivoGen) and recombinant murine IL-2 (20 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... Rnh1 was excised in Rnh1fl/fl Mx1-Cre+ mouse model by giving three rounds of 200 μg poly(I:C) (Invivogen) using intraperitoneal injections once every two days ...
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination test using PlasmoTest mycoplasma detection kit (InvivoGen) was done routinely for all the cell lines.
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bioRxiv - Molecular Biology 2020Quote: ... and tested for mycoplasma regularly (detection kit from Invivogen). HCT116-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene # 52962 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell cultures were certified mycoplasma-free (PlasmoTest kit, Invivogen).
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bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43 or 15 µg of SARS-CoV spike protein in 150 µL PBS together with 50 µL Addavax (MF59-like squalene adjuvant from InvivoGen) through intraperitoneally injection (i.p.) ...