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Citations for Invivogen :
151 - 197 of 197 citations for Kallikrein 3 PSA Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (InvivoGen ant-pm-1). For calcium imaging experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... The infected cultures were selected by sorting of RFP+ cells 3 days after transduction and expanded in supplemented media with puromycin (2.5µg/ml; InvivoGen) and blasticidin (1µg/ml ...
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bioRxiv - Immunology 2022Quote: 50μg biotinylated NS1 protein or 50μg (4-hydroxy-3-nitrophenyl)-acetyl(15)-OVA (NP-OVA) was adsorbed to 100μg alhydrogel alum (InVivoGen) in a total of 200μL per mouse for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Clones were selected with 3 μg/mL (and maintained with 1μg/mL) of Puromycin (Invivogen, #ant-pr-5b).
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bioRxiv - Cell Biology 2023Quote: ... co-transfected cells were selected in culture medium supplemented with 3 μg/ml puromycin (Invivogen, ant-pr-1), until selection was complete after about 48 h ...
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bioRxiv - Immunology 2020Quote: ... The lyophilized powder was combined with 300 µg of CpG (Murine CpG, ODN 1826, Porcine, Rabbit, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) which has been condensed with Polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Immunology 2022Quote: ... N = 3) were immunized with a mixture of 50 μg each of H1ssF and H10ssF formulated with AddaVax (Invivogen) in 1 ml via intramuscular route at weeks 0 ...
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bioRxiv - Neuroscience 2021Quote: ... with half media changes every 2-3 days with Neurobasal (ThermoFisher #21103049 supplemented with Primocin (InvivoGen #ant-pm-1), B-27 (ThermoFisher #17504044 ...
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bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
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bioRxiv - Microbiology 2020Quote: ... DNA ratio of 3:1 according to manufacturer’s instructions or with Poly(I:C) (LMW)/LyoVec™ (tlrl-picwlv, InvivoGen). The treatment was administered as indicated ...
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bioRxiv - Microbiology 2021Quote: ... were immunized with 3 ugs of purified RBD of SARS-CoV-2 mixed with 10 ugs of poly I:C (Invivogen) twice with 3 weeks interval (17 ...
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bioRxiv - Immunology 2023Quote: ... Adenosine 5’-triphosphate disodium salt (ATP, CAS: 987-65-5), poly(dA:dT), and nigericin (Nig, CAS: 28643-80-3) were from InvivoGen. Cross linker disuccinimidyl suberate (DSS ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cell Biology 2022Quote: ... Infected cells were selected by incubation in medium containing 200 µg/mL hygromycin B Gold or 3 µg/mL blasticidin S (InvivoGen) for 4 d ...
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bioRxiv - Immunology 2021Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Genetics 2020Quote: ... Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen). Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR ...
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bioRxiv - Pathology 2019Quote: ... Confluent mIMCD-3 cells were serum deprived for 16 hours and then stimulated with 1µg/mL LPS (Invivogen, tlrl-3pelps). RNAs and proteins were extracted 24 hours after stimulation.
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bioRxiv - Immunology 2021Quote: ... At the time of seeding 3 tumor fragments were additionally treated with either vehicle control (endotoxin-free water) or CpG ODN 2006 (InvivoGen) at 0.5ug/mL ...
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bioRxiv - Immunology 2022Quote: ... immunization with 50 µg TNP-KLH prepared with 1/3 volume of either alum or aluminum hydroxide gel (alhydrogel, Invivogen); 20 µg recombinantly produced trimer-stabilized HA (see below ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were seeded as 0.3*106 cells in 6-wells plate followed by treatment for 4 h with 333 nM of Torin 1 (InvivoGen) alone or in combination with 200 nM of Bafilomycin A1 for 1 h.
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bioRxiv - Microbiology 2022Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Immunology 2023Quote: ... the cells were pre-incubated with the respective antagonist as suggested by the manufacturer’s protocol (STING: H-151 for 1-2 hours and cGAS: RU.521 for 3 hours, both InvivoGen, USA) in cell growth medium ...
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bioRxiv - Immunology 2023Quote: ... alongside 100 μg mIgG1 anti-CD40 mAb (3/23, mouse IgG1 generated in-house as described previously[29, 30]) and the indicated doses of DMXAA(Invivogen), ADU-S100 was obtained from Oxeltis(Montpellier ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 ITGB1 KO cells were transduced with pWPI-ITGB1 and selected in cDMEM supplemented with 400 μg/ml G418 (Invivogen). S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Immunology 2023Quote: Immune responses were induced in 6-12-week-old male and female mice by subcutaneous immunization in the right FP with 5 μg (for HA experiments) or 10 μg (for hapten-carrier experiments) supplemented with 1/3 volume alhydrogel adjuvant (Invivogen). In S1pr2-Tomato mice ...
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bioRxiv - Immunology 2023Quote: ... whole splenocytes from OT-1 mice were electroporated on day 3 after initial activation with 1 µg/mL OVA 257-264 peptide (InvivoGen), and cells were further expanded for 4 days with 200 U/mL rhIL-2 (NCI) ...
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bioRxiv - Immunology 2023Quote: ... mice were immunized with 50 μg (day 3 analysis) or 100 μg (day 7 analysis) chicken ovalbumin (OVA) protein (InvivoGen) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Infected cells were selected by incubation in medium containing 3 µg/mL blasticidin S or 200 µg/mL hygromycin B Gold (InvivoGen) for 4 d.
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bioRxiv - Immunology 2020Quote: The cell lines A549-Dual (adenocarcinoma human alveolar basal epithelial cells) and RAW-Lucia ISG (RAW-mouse macrophages) (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... K18-hACE2 mice of both sexes were primed and boosted at 3 weeks intervals with S (10 µg/dose) or RBD (20 µg/dose) emulsified with AddaS03™ (InvivoGen) via intramuscular route ...
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bioRxiv - Cancer Biology 2023Quote: ... Regular testing for mycoplasma infection was conducted every 3–4 months using the PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Microbiology 2022Quote: ... The protocol using HEK-Blue™-hTLR2 and HEK-Blue™-hTLR4 reporter cell lines expressing human TLR2 and TLR4 (InvivoGen, San Diego, CA) was employed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the cationic lipid-based transfection reagent LyoVec and cyclic [G(2’,5’)pA(3’,5’)p] (2’3’-cGAMP) were obtained from Invivogen (San Diego, USA) and Lipofectamine2000 was obtained from ThermoFisher Scientific (Dreieich ...
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bioRxiv - Bioengineering 2021Quote: ... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (ant-pm-1, InvivoGen, San Diego, California, USA). Cultures were incubated at 37°C ...
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bioRxiv - Immunology 2022Quote: ... for 3 hr followed by (flagellin isolated from P. aeruginosa, 2 or 5 µg/mL) for 3 hr using FLA-PA Ultrapure (InvivoGen, tlrl-pafla) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were treated with 400 µM FFA and 3 µM HSG4112 in the presence or absence of bafilomycin A1 (Invivogen, tlrl-baf1). Cellular localization of LC3B was observed using a Carl Zeiss Confocal LSM710 Meta microscope and the images were processed with the software supplied by the manufacturer (Carl Zeiss ...
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bioRxiv - Immunology 2020Quote: ... with penicillin-streptomycin (100 units/mL) and activated using 3 µM CpG (ODN 7909) (tlrl-2006-1, Invivogen, San Diego, CA, USA). Cells were cultured in 200 µl medium for 1-8 days using round bottom 96-well plates ...
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were routinely confirmed to be negative for mycoplasma infection every 3–4 months using PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen, San Diego, CA) for 15 min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Levels of SEAP were detected in the culture media after 3 hours incubation of supernatants with Quanti-Blue solution (InvivoGen, San Diego, CA, USA) at 650nm wavelength by ELISA reader.
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bioRxiv - Genetics 2023Quote: ... the following were added directly to worm plates 3 days after injection: HygR selection −100 ul of 20 mg/ml Hygromycin (HygroGold™ InvivoGen, San Diego, CA) or Hygromycin B ...
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bioRxiv - Immunology 2021Quote: ... Mice (8 mice per group) were intramuscularly injected twice at four-week intervals with each VLPs (HA content=3 µg) with AddaVax™ adjuvant (Invivogen, San Diego, CA, USA) (Figure 2) ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) Primocin™ (InvivoGen, San Diego, CA; ant-pm-1) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...