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Citations for Invivogen :
151 - 200 of 248 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2023Quote: ... was fused to the Fc region (CH2 and CH3 domains) and hinge region of the human IgG1 heavy chain in the pFUSE-Fc1 vector (InvivoGen). Jag1ECD-Fc protein was expressed in Expi293F cells grown in Expi293F expression medium at 37°C in an 8% CO2 incubator with constant shaking ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: The human monocytic THP-1 cell line (ATCC TIB-202) and the THP-1 DualTM reporter cell (InvivoGen, Thpd-nfis) were culture in RPMI supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Genetics 2020Quote: ... IFN I response was induced by high molecular weight poly(I:C) complexes with LyoVec transfection reagent (Invivogen, tlrl-piclv, 500 ng/ml) or with a combination of poly(I:C)/LyoVec and interferon beta (PeproTech ...
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bioRxiv - Neuroscience 2021Quote: ... were mixed well and heated to 50°C (or 65-70°C in the case of Invivogen high molecular weight poly I:C) in order to solubilize and melt dsRNA and then cooled to room-temperature to allow re-annealing before freezing in 1 ml aliquots and storing at −20 °C ...
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bioRxiv - Immunology 2020Quote: ... IFN I response was induced by high molecular weight poly(I:C) complexes with LyoVec transfection reagent (Invivogen, tlrl-piclv, 500 ng/ml), in combination with IFNβ (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... HEK293-C34 cells were gifted by Y Matsuura at Osaka University and maintained in high-glucose DMEM (Nacalai Tesque) containing 10% FBS and 10 μg/mL blasticidin (solution) (InvivoGen, California, USA), and the exogenous expression of ACE2 and TMPRSS2 was induced by the addition of doxycycline hydrochloride (1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The lyophilized powder was combined with 300 µg of CpG (Murine CpG, ODN 1826, Porcine, Rabbit, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) which has been condensed with Polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Physiology 2021Quote: ... Duodenal or jejunal segments (∼6 cm) were isolated and placed into falcon tubes containing cold DMEM (high glucose, 4.5 g/l) containing Primocin (Invivogen, cat# ant-pm-1). Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: Five zebrafish housed in 400-mL beakers containing 200mL sterile tank water were immersed in high molecular weight polyinosine-polycytidylic acid (HMW poly I:C, InvivoGen Cat.# tlrl-pic-5) (50µg/mL final concentration in 200mL tank water ...
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bioRxiv - Immunology 2024Quote: ... mice were intradermally injected with 50 μg of neoantigen or control peptide and/or 100 μg high molecular weight VacciGrade polyinosinic-polycytidylic acid (poly(I:C) (Invivogen Cat. No. vac-pic)) ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
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bioRxiv - Developmental Biology 2023Quote: ... or Mycostrip detection kit (Invivogen). Cells were routinely screened for indicatiors of pluripotency OCT4 ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Microbiology 2023Quote: ... A549-derived cells were transfected with 500 ng of poly(I:C) (a mix of high and low molecular weight, ratio 1:1, InvivoGen, tlrl-picw and tlrl-pic) using lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: The cell lines A549-Dual (adenocarcinoma human alveolar basal epithelial cells) and RAW-Lucia ISG (RAW-mouse macrophages) (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... virgin female mice were injected intraperitoneally (IP) with a low dose of high molecular weight (HMW) Poly(I:C) dsRNA (InvivoGen, San Diego, CA. Cat# tlrl-pic). At 2.5hrs or 4hrs post-injection ...
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bioRxiv - Microbiology 2022Quote: ... The protocol using HEK-Blue™-hTLR2 and HEK-Blue™-hTLR4 reporter cell lines expressing human TLR2 and TLR4 (InvivoGen, San Diego, CA) was employed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... determined using the Endotoxin Detection kit (InvivoGen).
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Cancer Biology 2021Quote: ... examined for mycoplasma regularly (detection kit from Invivogen). A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell cultures were routinely tested for mycoplasma contamination using Universal Mycoplasma Detection Kit (ATCC, #30-1012K) and PlasmoTest Mycoplasma Detection Kit (Invivogen, #rep-pt1).
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bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma contamination test using PlasmoTest mycoplasma detection kit (InvivoGen) was done routinely for all the cell lines.
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bioRxiv - Molecular Biology 2020Quote: ... and tested for mycoplasma regularly (detection kit from Invivogen). HCT116-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene # 52962 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell cultures were certified mycoplasma-free (PlasmoTest kit, Invivogen).
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bioRxiv - Immunology 2019Quote: ... a Mouse TLR Agonist kit was purchased from Invivogen and BMDMs were treated with each ligand for 16 hours ...
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bioRxiv - Immunology 2021Quote: ... Mouse IFN-α was measured by a bioluminescence kit (InvivoGen). All assays were performed on cell free supernatants according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... IFNβ was measured with a bioluminescent ELISA kit (LumiKine, Invivogen). For experiments involving blocking antibodies (Abs) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely tested for mycoplasma using Mycostrip kit (Invivogen).
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bioRxiv - Cell Biology 2020Quote: ... Testing for mycoplasma contamination was performed bimonthly using the PlasmoTest kit (InvivoGen).
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bioRxiv - Cell Biology 2019Quote: ... Testing for mycoplasma contamination was performed bimonthly using the PlasmoTest kit (InvivoGen). Plasmids were transfected using either X-tremeGENE 9 (Roche) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were tested regularly for mycobacterium contamination using the PlasmoTest kit (Invivogen) and authenticated by the source ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lines were confirmed mycoplasma-free using the PlasmoTest mycoplasma kit (Invivogen). Cell lines were passaged every 4-6 days and MIN6 were detached with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... RAW-Blue ISG and Quantified-Blue assay kit were obtained from Invivogen, Ltd ...
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bioRxiv - Cell Biology 2024Quote: ... Lipofectamine 3000 transfection kit was purchased from InvivoGen (San Diego, CA, USA). Phospho-IRF3(Ser396 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were regularly tested for mycoplasma contamination using PlasmoTestTM Mycoplasma Detection Kit (InvivoGen).
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bioRxiv - Cancer Biology 2021Quote: ... and tested negative for Mycoplasma using PlasmoTest-Mycoplasma Detection Kit (InvivoGen, rep-pt1). Experiments were performed within the first few passages after thawing frozen cell stocks ...
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bioRxiv - Cell Biology 2021Quote: ... All cells were routinely checked for mycoplasma contamination using PlasmoTest™ Kit (InvivoGen).
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Ferroportin Depletes Iron Needed for Cell Cycle Progression in Head and Neck Squamous Cell CarcinomabioRxiv - Cancer Biology 2022Quote: ... Cells were screened for mycoplasma using PCR or MycoStripTm mycoplasma detection kit (Invivogen).
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bioRxiv - Cell Biology 2023Quote: ... and were routinely checked for mycoplasma (PlasmoTest Mycoplasma detection kit, InvivoGen, #rep-pt1).
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bioRxiv - Immunology 2024Quote: ... ODN1826 and ODN2395 were from a mouse TLR9 agonist kit (Invivogen, tlrl-kit9m). Poly(I:C ...
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bioRxiv - Cancer Biology 2024Quote: ... using the PlasmoTestTM Mycoplasma Detection Kit (rep-pt1; InvivoGen, San Diego, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... They were routinely checked for mycoplasma (PlasmoTest™-Mycoplasma Detection Kit, InvivoGen, #rep-pt1) and underwent cell authentification by short tandem repeat analysis (powerplex16 HS kit ...
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bioRxiv - Cell Biology 2022Quote: ... All cells were tested for mycoplasma contamination every two weeks with PlasmoTest kit (InvivoGen). Reagents used for transfections were ...