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Citations for Invivogen :
101 - 150 of 150 citations for Human C17orf112 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Microbiology 2023Quote: ... human cardiac fibroblasts in 96-well plate were transfected with 62 ng of high molecular weight poly(I:C) (InvivoGen) using TransIT-mRNA Transfection Kit (Mirus Bio) ...
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bioRxiv - Biochemistry 2023Quote: HKB11 PD-1 cells were used as stable pool generated by transfecting HKB11 cells with pMAX vector41 encoding human PD-1 (UniProt Q15116) and selection with 0.8 mg/mL geneticin (Invivogen).
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bioRxiv - Immunology 2021Quote: ... variable genes were inserted into plasmids encoding the constant region for the heavy chain (pFUSEss-CHIg-hG1, Invivogen) and light chain (pFUSE2ss-CLIg-hl2 ...
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bioRxiv - Cell Biology 2020Quote: Rescue experiments were performed by transfecting clonal B16F1 CRTC3 KO cells with pSelect-puro-mcs plasmid (psetp_mcs, Invivogen) carrying full length CRTC3 ...
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bioRxiv - Physiology 2023Quote: Mouse FGL1 cDNA sequences (full length, N-terminal domain, globular domain) were cloned into pFUSEN-hG2Fc plasmid (InvivoGen) with the following modifications ...
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Bioengineering 2022Quote: Non-glycosylated monoclonal human IgG1 antibody against human EGFR (hegfr-mab12, lot: EG12-39-01) and isotype control -Human IgG1 (bgal-mab1, lot: BG1-41-01) were purchased from InvivoGen. Human EGFR (Research Grade Cetuximab Biosimilar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2023Quote: ... was fused to the Fc region (CH2 and CH3 domains) and hinge region of the human IgG1 heavy chain in the pFUSE-Fc1 vector (InvivoGen). Jag1ECD-Fc protein was expressed in Expi293F cells grown in Expi293F expression medium at 37°C in an 8% CO2 incubator with constant shaking ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: The human monocytic THP-1 cell line (ATCC TIB-202) and the THP-1 DualTM reporter cell (InvivoGen, Thpd-nfis) were culture in RPMI supplemented with 10% heat-inactivated fetal calf serum ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Microbiology 2019Quote: ... in 12-well plates were transfected with 0.2 µg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Freestyle™ 293-F were transfected with pUNO1-Spike-dfur expression vectors (Spike and SpikeV1 to V11 plasmids, Invivogen) (1.2 µg plasmid DNA per 106 cells ...
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bioRxiv - Microbiology 2023Quote: ... in 24-well plates were transfected with 0.1 µg of plasmid DNA per well using LyoVec transfection reagent (InvivoGen), according to manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... The lyophilized powder was combined with 300 µg of CpG (Murine CpG, ODN 1826, Porcine, Rabbit, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) which has been condensed with Polyethyleneimine (PEI ...
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bioRxiv - Microbiology 2023Quote: The ADCC reporter assay was performed by using Jurkat-Lucia NFAT-CD16 cells (human FcγRIII, V158) (Invivogen, Cat# jktl-nfat-cd16) as effector cells according to the manufacturer’s instruction with modifications ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Microbiology 2022Quote: ... five different pseudotypes were generated using expression plasmids of respective spike variants: for prototype B.1/D614G as before (1) or sourced from Invivogen for VOCs Beta (Cat ...
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bioRxiv - Genetics 2019Quote: The 24 and 48bp homology arm CMV/GFP/Zeocin resistance knock-in cassettes were generated by designing PCR primers complementary to the psiRNA-SV40 Early PolyA GFPzeo plasmid (Invivogen) flanked by the 48 base pairs of homology and the UgRNA sequence separated by a 3bp spacer ...
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bioRxiv - Molecular Biology 2019Quote: The enhancer/promoter region of the chicken vitellogenin II gene spanning nucleotides −638 to −1 was cloned into a CpG-free firefly luciferase plasmid (pCpGL-basic, InvivoGen) using HindIII and NcoI ...
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bioRxiv - Cell Biology 2020Quote: We generated dual expression barcoding vectors by inserting pairs of barcoding protein sequences into the pVITRO1-hygro-mcs plasmid (InvivoGen), which contains two multiple cloning sites MCS1 and MCS2 (Supplementary Fig ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human antibodies were expressed in HEK-293T cells following transient transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from Invivogen. Recombinant antibodies were purified using Protein A sepharose (IBA ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells were transfected with the respective S protein expression plasmids for the delta variant (Cat. No. plv-spike-v8, Invivogen) and the omicron variant (A67V ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Microbiology 2020Quote: ... or 7.7G6 mAb were produced in HEK-293T cells following transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from InvivoGen. Human antibodies were purified from cell culture supernatants using Protein-A affinity chromatography ...
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bioRxiv - Cancer Biology 2020Quote: ... transient transfection of HEK-293FT cells with packaging and lentiviral vector plasmids were performed using LENTI-Smart INT kit (InvivoGen) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... eGFP alone or POP-eGFP plasmids (600 ng and 1200 ng) were co-transfected with inflammasome-mCherry plasmids (300 ng) at 70% confluence using lipofectamine 2000 (Invivogen). After 16 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lines transfected with the pMOT-4H plasmid (Oberholzer et al 2006) were grown with 25 ug/ml of hygromycin B Gold (Invivogen).
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bioRxiv - Immunology 2023Quote: ... FreeStyleTM 293-F cells were co-transfected with the specified M2e-MAbs heavy and light chain variable region plasmids (InvivoGen) using Polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cell Biology 2021Quote: ... or photoactivatable GFP-α-tubulin (plasmid provided by Alexey Khodjakov) were cultured in DMEM supplemented with 1 mg/mL G418 (InvivoGen). All cell lines were grown in medium supplemented with 10% FBS (HyClone) ...
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bioRxiv - Immunology 2022Quote: ... were generated by transfecting OKT3-2A-Hygromycin_pEK plasmid as previously described and selected for with 0.4mg/mL hygromycin (InvivoGen, ant-hg-1) (12) ...
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bioRxiv - Immunology 2023Quote: ... To produce pseudoviruses expressing the S protein from different variants the following plasmids were used: Alpha (B.1.1.7) (InvivoGen, plv-spike-v2); Beta (B.1.351 ...
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bioRxiv - Immunology 2020Quote: The cell lines A549-Dual (adenocarcinoma human alveolar basal epithelial cells) and RAW-Lucia ISG (RAW-mouse macrophages) (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
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bioRxiv - Microbiology 2022Quote: ... The protocol using HEK-Blue™-hTLR2 and HEK-Blue™-hTLR4 reporter cell lines expressing human TLR2 and TLR4 (InvivoGen, San Diego, CA) was employed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cas9+mCherry+ RMS cells were then transiently transfected with pLentiguide-PURO plasmid and selected with 1 µg/ml Puromycin (InVivoGen, #ant-pr-1) for two days ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...