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Citations for Invivogen :
1251 - 1300 of 1321 citations for 7 Chloro 3 4 2 hydroxyethyl 1 piperazinyl 1 2 propoxyethyl pyrido 3 4 b pyrazin 2 1H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Biochemistry 2022Quote: ... K562s were transfected according to manufacturer’s protocol with EGFR using pre-packaged lentiviral particles (G&P Biosciences) and selected for EGFR expression by culture in 1 μg/mL puromycin (InVivoGen). K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences LTV-CD20 ...
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bioRxiv - Cancer Biology 2022Quote: Mouse colonic organoids were maintained in maintained in advanced DMEM/F-12 supplemented with 1X Primocin (Invivogen #ant-pm-1), 10mM HEPES ...
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bioRxiv - Immunology 2022Quote: WT and LRBA-KO HEK293T cells were plated on 12 mm cover slides in a 24-wells plate and then treated for 1 h with 50 µM Rapamicin (Invivogen), 333 nM Torin 1(Invivogen) ...
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bioRxiv - Microbiology 2022Quote: VeroE6/TMPRSS2 (JCRB 1819) cells (Matsuyama et al., 2020) were propagated in the presence of 1 mg/ml geneticin (G418; Invivogen) and 5 μg/ml plasmocin prophylactic (Invivogen ...
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bioRxiv - Molecular Biology 2023Quote: ... were then stably integrated into this cell line by Cas9-mediated integration into the AAVS1 locus by simultaneous nucleofection of plasmids containing either targeting sgRNAS (AAVS1-T2 and AAVS1-T2, Table S3) and spCas988 and selected using 1 µg/mL Puromycin (Invivogen) to generate stable integrants ...
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bioRxiv - Immunology 2022Quote: ... with 5 µg H7 HA-12D adjuvanted with 150 µg alum alone or 150 µg alum and 1 µg CpG (ODN 1826, InvivoGen) via subcutaneous injection on days 0 and 21 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were grown until confluency and reseeded into 6-well dishes prior to addition of 1 µg/ml puromycin (InvivoGen) and 5 µg/ml blasticidin (InvivoGen) ...
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bioRxiv - Neuroscience 2023Quote: ... The culture media was also supplemented with 0.2% (1:500 dilution from a 5 μg/ml working concentration) Plasmocin™ Prophylactic (ant. mpp; InvivoGen). The day of plating from cryopreservation was allocated as day 0 and 50% media changes were carried out every 2-3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells expressing the TCR reporter construct were maintained in culture with 10 µg/ml blasticidin (Invivogen ant-bl-1). For TCR expression ...
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Genomics 2022Quote: ... while material that was collected with washing of the cell strainer surface with ice cold PBS with 1% (v/v) primocin (InvivoGen) was referred to as jejunal epithelial cell fraction two (IEC-2) ...
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Pathology 2023Quote: ... The present study used the MTA model using 1 µg/ml unmethylated CpG oligonucleotide (CpG ODN: InvivoGen, San Diego, CA) or P ...
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bioRxiv - Cancer Biology 2024Quote: ... OSCC cell lines were than infected with the lentiviruses in the supernatants and the pools were selected using 1 µg/ml puromycin (Invivogen) for 5 days ...
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bioRxiv - Microbiology 2024Quote: ... Cells transduced with lentiviruses encoding for the different gRNAs were passaged in selection medium containing 10 µg/ml blasticidin and 5 µg/ml puromycin (ant-pr-1, InvivoGen) to obtain polyclonal cell populations lacking either GSDMD or GSDME ...
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bioRxiv - Immunology 2023Quote: ... Cat# tlrl-hprna), LyoVec (Cat# lyec-1) and dispersible whole glucan particles (WGP, Cat# tlrl-wgp) were purchased from InvivoGen. Recombinant murine IFN-β1 (Cat# 581304 ...
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
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bioRxiv - Genomics 2023Quote: ... Cells were then imaged in the imaging buffer (0.5 μg/ml DAPI, 10 μg/ml Fungin (InvivoGen ant-fn-1) in PBS ...
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bioRxiv - Genetics 2024Quote: ... the pooled mixture of cells were expanded and subsequently maintained in D10-dox containing 1 μg/mL puromycin (InvivoGen, ANTPR).
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bioRxiv - Immunology 2021Quote: ... BMDCs were surface-labeled with fluorochrome-coupled antibodies to the following antigens (all 1:400): MHCII-v450 (Invivogen, 48-5321-82), CD11b-PerCP-Cy5.5 (Invivogen ...
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
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bioRxiv - Neuroscience 2021Quote: ... or the same amount of IgG negative control antibody (Bio-Rad, MCA6004GA for MARCO and Invivogen, bgal- mab10-1 for TLR2) was administered in the same needle with IL-33.
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bioRxiv - Cell Biology 2021Quote: ... or photoactivatable GFP-α-tubulin (plasmid provided by Alexey Khodjakov) were cultured in DMEM supplemented with 1 mg/mL G418 (InvivoGen). All cell lines were grown in medium supplemented with 10% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2020Quote: ... reporter gene under the control of an IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites (Invivogen).
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bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
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bioRxiv - Immunology 2022Quote: ... were generated by transfecting OKT3-2A-Hygromycin_pEK plasmid as previously described and selected for with 0.4mg/mL hygromycin (InvivoGen, ant-hg-1) (12) ...
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bioRxiv - Bioengineering 2024Quote: ... this clone was used to generate a stable pool harboring the human glycosyltransferase gene ST6GAL1 via transfection with a plasmid containing the coding sequence of human beta-galactoside alpha-2,6-sialyltransferase 1 isoform a (hSTGAL1) (GenBank NP_001340845.1) and expressed from a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a derivative of pcDNA3.1(+ ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Molecular Biology 2019Quote: ... Then digested tissues were triturated with a pipette in plating medium (DMEM with 10% FBS and 100 μg/mL antimicrobial agent Primocin™ (ant-pm-1, Invivogen, USA). Dissociated neurons were immediately plated onto 35 mm dishes coated with 50 μg/mL poly-D-lysine (P6407 ...
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bioRxiv - Immunology 2021Quote: ... about 0.5cm² and incubated at 4C in an antibiotic mixture consisting of 1X PBS +100ug/mL Normocin (Invivogen Cat# ant-nr-1), 50ug/mL Gentamicin (Amresco ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
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bioRxiv - Immunology 2022Quote: ... stimuli were as follows: 1×108 pfu/well of purified phage preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). The TLR3 signalling inhibitor (EMD Millipore ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Immunology 2022Quote: A single dose consisted of 10 μg of peptide (peptide stock is 10 mg/mL resuspended in DMSO) was added to 25 μg cyclic-di-GMP (stock is 1 mg/mL resuspended in PBS) (Invivogen tlrl-nacdg) and PBS added to a final volume of 50 μL ...
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bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
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bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Bioengineering 2023Quote: ... the mice were intratumorally injected with 40 μg of STINGΔTM protein mixed with 1 μg of 2’3’-cGAMP (InvivoGen, Cat # tlrl-nacga23-02) in 25 μL sterile PBS ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the library were measured by mCherry flow cytometry and selected with 1 μg/ml puromycin (Invivogen, ant-pr). The selected cells were expended in culture for 13-14 doublings to obtain enough number of cells for xenograft experiments.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...