Labshake search
Citations for Invivogen :
1251 - 1300 of 1318 citations for 7 BROMO 2 1 3 BENZOTHIADIAZOLE 4 SULFINIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... The culture media was also supplemented with 0.2% (1:500 dilution from a 5 μg/ml working concentration) Plasmocin™ Prophylactic (ant. mpp; InvivoGen). The day of plating from cryopreservation was allocated as day 0 and 50% media changes were carried out every 2-3 days ...
-
bioRxiv - Molecular Biology 2023Quote: ... were then stably integrated into this cell line by Cas9-mediated integration into the AAVS1 locus by simultaneous nucleofection of plasmids containing either targeting sgRNAS (AAVS1-T2 and AAVS1-T2, Table S3) and spCas988 and selected using 1 µg/mL Puromycin (Invivogen) to generate stable integrants ...
-
bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells expressing the TCR reporter construct were maintained in culture with 10 µg/ml blasticidin (Invivogen ant-bl-1). For TCR expression ...
-
bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
-
bioRxiv - Genomics 2022Quote: ... while material that was collected with washing of the cell strainer surface with ice cold PBS with 1% (v/v) primocin (InvivoGen) was referred to as jejunal epithelial cell fraction two (IEC-2) ...
-
bioRxiv - Immunology 2023Quote: ... Cat# tlrl-hprna), LyoVec (Cat# lyec-1) and dispersible whole glucan particles (WGP, Cat# tlrl-wgp) were purchased from InvivoGen. Recombinant murine IFN-β1 (Cat# 581304 ...
-
bioRxiv - Immunology 2023Quote: ... wild-type BALB/c female mice were sensitized intraperitoneally twice at 7-day intervals with either PBS (Group A) or 40 μg of OVA (Group B-F) together with 1 mg of aluminum hydroxide adjuvant (InvivoGen) on day 0 and day 7 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
-
bioRxiv - Pathology 2023Quote: ... The present study used the MTA model using 1 µg/ml unmethylated CpG oligonucleotide (CpG ODN: InvivoGen, San Diego, CA) or P ...
-
bioRxiv - Bioengineering 2024Quote: ... K562s were transfected according to manufacturer’s protocol with EGFR using pre-packaged lentiviral particles (G&P Biosciences) and selected for EGFR expression by culture in 1 µg/mL puromycin (InVivoGen). K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences ...
-
bioRxiv - Genetics 2024Quote: ... and nitrate salts are as described in the appendix of reference (Kafer E, 1977) supplemented with 1% sorbitol and 20 mg/liter phleomycin (InvivoGen) after adjusting the pH to 7.0 ...
-
bioRxiv - Immunology 2021Quote: ... BMDCs were surface-labeled with fluorochrome-coupled antibodies to the following antigens (all 1:400): MHCII-v450 (Invivogen, 48-5321-82), CD11b-PerCP-Cy5.5 (Invivogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
-
bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
-
bioRxiv - Neuroscience 2021Quote: ... or the same amount of IgG negative control antibody (Bio-Rad, MCA6004GA for MARCO and Invivogen, bgal- mab10-1 for TLR2) was administered in the same needle with IL-33.
-
bioRxiv - Cell Biology 2021Quote: ... or photoactivatable GFP-α-tubulin (plasmid provided by Alexey Khodjakov) were cultured in DMEM supplemented with 1 mg/mL G418 (InvivoGen). All cell lines were grown in medium supplemented with 10% FBS (HyClone) ...
-
bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
-
bioRxiv - Pathology 2020Quote: ... reporter gene under the control of an IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites (Invivogen).
-
bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
-
bioRxiv - Immunology 2022Quote: ... were generated by transfecting OKT3-2A-Hygromycin_pEK plasmid as previously described and selected for with 0.4mg/mL hygromycin (InvivoGen, ant-hg-1) (12) ...
-
bioRxiv - Bioengineering 2024Quote: ... this clone was used to generate a stable pool harboring the human glycosyltransferase gene ST6GAL1 via transfection with a plasmid containing the coding sequence of human beta-galactoside alpha-2,6-sialyltransferase 1 isoform a (hSTGAL1) (GenBank NP_001340845.1) and expressed from a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a derivative of pcDNA3.1(+ ...
-
bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
-
bioRxiv - Bioengineering 2024Quote: ... Results were collected 24 hours after treatment using a mouse IFN-β solid-phase sandwich ELISA kit (Invivogen Cat#42400-1) on cell supernatants following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... Results were collected 24 hours after treatment using a mouse IFN-β solid-phase sandwich ELISA kit (Invivogen Cat#42400-1) on cell supernatants following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... GFP only) were cultured in the same medium and selected by adding 100 μg/ml of hygromycin B (InvivoGen, ant-hg-1) to the media ...
-
bioRxiv - Molecular Biology 2021Quote: ... Stable transformants were maintained in media supplemented with blasticidin and 50 μg/ml Hygromycin B Gold (InvivoGen, cat n°ant-hg-1).
-
bioRxiv - Molecular Biology 2019Quote: ... Then digested tissues were triturated with a pipette in plating medium (DMEM with 10% FBS and 100 μg/mL antimicrobial agent Primocin™ (ant-pm-1, Invivogen, USA). Dissociated neurons were immediately plated onto 35 mm dishes coated with 50 μg/mL poly-D-lysine (P6407 ...
-
bioRxiv - Immunology 2021Quote: ... about 0.5cm² and incubated at 4C in an antibiotic mixture consisting of 1X PBS +100ug/mL Normocin (Invivogen Cat# ant-nr-1), 50ug/mL Gentamicin (Amresco ...
-
bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
-
bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
-
bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
-
bioRxiv - Immunology 2022Quote: ... stimuli were as follows: 1×108 pfu/well of purified phage preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). The TLR3 signalling inhibitor (EMD Millipore ...
-
bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
-
bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the library were measured by mCherry flow cytometry and selected with 1 μg/ml puromycin (Invivogen, ant-pr). The selected cells were expended in culture for 13-14 doublings to obtain enough number of cells for xenograft experiments.
-
bioRxiv - Immunology 2022Quote: A single dose consisted of 10 μg of peptide (peptide stock is 10 mg/mL resuspended in DMSO) was added to 25 μg cyclic-di-GMP (stock is 1 mg/mL resuspended in PBS) (Invivogen tlrl-nacdg) and PBS added to a final volume of 50 μL ...
-
bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
-
bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
-
bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
-
bioRxiv - Bioengineering 2023Quote: ... the mice were intratumorally injected with 40 μg of STINGΔTM protein mixed with 1 μg of 2’3’-cGAMP (InvivoGen, Cat # tlrl-nacga23-02) in 25 μL sterile PBS ...
-
bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
-
bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
-
bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
-
bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... dTomato-positive cells were sorted on a BD FACS Aria III using BD FACSDiva 8.0.1 Software using a 100 µm nozzle and cultured on Martigel-coated plates in mTeSR Plus supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and 25 µm Y-27632 ...
-
bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...