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Citations for Invivogen :
1251 - 1300 of 1368 citations for 4 3 2 Chloroethyl 3 nitrosoureido tetrahydro 2H thiopyran 1 1 dioxide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 1 mg/kg of lipopolysaccharide (LPS endotoxin from E. coli 0111:B4 ultra-pure; InvivoGen, San Diego, CA) dissolved in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... T47D and MCF7 cells were transduced with the lentiviral particles and stably expressing cells were generated after selection using G418 (InvivoGen ant-gn-1) for two weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... 293T cells transduced to stably express TIM1 (TIM1-293T) or mock transduced (Mock-293T) were maintained in the medium supplemented with 1 μg/mL puromycin (InvivoGen, Cat# ant-pr).
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bioRxiv - Cell Biology 2024Quote: ... was a gift from Kristen Brennard and stably expressed in RPE1 cells using puromycin selection (10 ug/mL, Invivogen Cat#ant-pr-1). CA-YAP and dCas9-expressing cells were generated using third-generation lentiviral transduction ...
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bioRxiv - Cell Biology 2024Quote: ... Stable cell lines generated in this background were cultured with 5 µg/ml of blasticidin and 200 µg/ml of hygromycin B (Invivogen #ant-hg-1). To minimise background expression of the transgene ...
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bioRxiv - Cell Biology 2024Quote: ... For GSK3 knockout/knockdown cells were generated by transfecting 293T cells with the corresponding lentiCRISPR v2 plasmid with jetPRIME (Polyplus-transfection, Cat# 101000046) and selected with puromycin (InvivoGen ant-pr-1) (1 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in complete RPMI-1640 medium and were transfected with 1 mg/ml cGAS agonist (G3-ended Y-form Short DNA) (InvivoGen, San Diego, USA) and LyoVec™ (InvivoGen ...
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bioRxiv - Biochemistry 2021Quote: PMA-differentiated THP1 macrophages in 24-well plates were pretreated with fatty acids (2.5 μM and 10 μM) for 30 min prior to stimulation with 4 μg/mL cGAMP (InvivoGen) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl UV-treated sample was inoculated onto 4×104 cells/ well of HEK-Blue IFNα/β cells (InvivoGen) in 96-well plates ...
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bioRxiv - Genetics 2023Quote: ... Ganciclovir selection was conducted for 4 days under 10 μM ganciclovir/culture medium (#sud-gcv; InvivoGen, San Diego, CA) after G418 selection or 8 days after single-cell sorting ...
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bioRxiv - Cell Biology 2020Quote: ... Agonist were used as following concentrations: 5’ppp-dsRNA (2 μg/ml, tlrl-3prna, Invivogen) and 2’3’-cGAMP (10 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... transfection reagent (vehicle) and 2 μg/ml or 10 μg/ml ultrapure LPS-B5 (InvivoGen) prepared from E ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2023Quote: ... 2 μg of M2ex3 antigen + 40 μg CpG (oligonucleotide 1826, a TLR9 agonist from InvivoGen), or 2 μg of M2ex3 + 40 μg STING agonist (2’3’-c-di-AM(PS)2(Rp,Rp) ...
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bioRxiv - Immunology 2022Quote: ... the medium was refreshed with RPMI-1640 complete medium containing 2 μg/ml puromycin (Invivogen). The medium was refreshed every 2-3 days for two weeks and identified the transfection efficiency by immunoblot.
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bioRxiv - Immunology 2023Quote: ... with 2% NuSerum medium (29) supplemented with Primocin (50 µg/ml, (InvivoGen, San Diego, CA), and retinoic acid (1 x 10−8 M ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% advanced fetal bovine serum (FBS, Capricorn) and 2 µg/mL puromycin (InvivoGen). For AAV production ...
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bioRxiv - Molecular Biology 2022Quote: ... aerogenes suspension (OD600 = 2) in SorMC and the indicated concentration of Hygromycin B Gold (InvivoGen) at 2×105 cells for AX4 or 1×105 cells for NC28.1 ...
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bioRxiv - Microbiology 2024Quote: ... Transduced cells were maintained and selected in the presence of 2 µg/mL puromycin (Invivogen). Validating knockdown cell lines at the functional level were carried out by incubating PMA/THP-1 cells with a variety of RLR or TLR ligands for 24-48 hours and measuring IP-10 in the culture supernatants via ELISA (described below) ...
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bioRxiv - Immunology 2024Quote: ... with 2% NuSerum medium (68) supplemented with Primocin (50 µg/ml, (InvivoGen, San Diego, CA), and retinoic acid (1 x 10−8 M ...
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bioRxiv - Immunology 2024Quote: ... the following commercial antibodies were used as positive controls: anti-Flagellin (mabg-flic-2, InvivoGen), anti-DNA (MAB1293 ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Immunology 2021Quote: ... NLRP3-/- iBMDMs or NLRP3-/- iBMDMs reconstituted with mScarlet-tagged NLRP3 were seeded at 0.5×106 cells/well on a 12-well tissue culture plate and on the next day treated or not with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 1% (v/v) nonessential amino acid (Multicell Wisent Inc., St-Bruno, QC, Canada) and 5μg/mL of Plasmocin® (Invivogen, San Diego, CA, USA), to prevent mycoplasma contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... The EGFP-fused lineage barcode was confirmed to exist as a single copy in the genome and to be highly expressed after blasticidin selection (15 g/ml, InvivoGen, no. ant-bl-1) and flow cytometry sorting ...
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated for 24 hours with 1×108 pfu/well of purified Pf4 phage or equivalent volume ΔPf4 preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). Cells were centrifuged at 300 x g for 5 minutes and supernatant was collected and used for cytokine profiling through Luminex (The Human Immune Monitoring Center ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of cell mixture was transferred to a white opaque 96-well plate and treated with 50 μL of QUANTI-Luc 4 Lucia/Gaussia (InvivoGen) and immediately read using luminescence at 0.1 second read time ...
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bioRxiv - Immunology 2024Quote: ... with WT or Sulf2+/- antigen-presenting cells (BMDM or DCs) activated with LPS (100 ng/ml LPS, 4 h, InvivoGen). As a source of antigen ...
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bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 days after transfection the cells were transferred to 10 cm dish and 4-5 days after transfection cells were selected with 100 μg/ml Hygromycin (InvivoGen). All CRIS-PITCh plasmids are summarized in Table S1.
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bioRxiv - Developmental Biology 2021Quote: ... Mice were injected with the following drugs and euthanized after 2 hours: Poly(I:C) HMW (Invivogen), IP injection 10mg/kg (Pietras et al. ...
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bioRxiv - Biochemistry 2020Quote: ... Infected cells were selected for by the addition of 2 μg/mL puromycin (InvivoGen; ant-pr). Expression was verified by SDS-PAGE and/or BN-PAGE analysis.
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bioRxiv - Cell Biology 2020Quote: ... cells infected with shRNA encoding virus were selected in puromycin (2 μg/mL, InvivoGen, ant-pr) and used 4 days post infection.
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells were stably transfected with 2 μg of pUNO1-hOSM expression construct (InvivoGen), using TurboFect™ followed by Blasticidin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 2 hours with or without TLR2 blocking antibody (Invivogen cat.# mab2-mtlr2) followed by incubation with CEP or Pam3CSK4 (Invivogen cat.# tlrl-pms ...
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bioRxiv - Immunology 2022Quote: ... filter sterilised and tested for endotoxin content using the HEK-Blue LPS detection kit 2 (InvivoGen) and found to have <0.002 EU endotoxin per mg of protein ...
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bioRxiv - Immunology 2023Quote: ... MEFs or BMDMs were transfected with 2 µg/ml Interferon Stimulatory DNA (ISD, tlrl-isdn, InvivoGen) complexed with Lipofectamine 2000 (11668019 ...
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bioRxiv - Immunology 2024Quote: ... filter sterilized and assayed for LPS levels using the HEK-Blue LPS Detection kit 2 (InvivoGen). All proteins used in these experiments contain < 0.1 EU/ug LPS.
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bioRxiv - Immunology 2024Quote: ... the cell suspensions were stimulated with 2 µg/mL cytosine-phosphate-guanosine oligodeoxynucleotide (CpG ODN; InvivoGen), 50 ng/ml phorbol myristate acetate (PMA ...
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Immunology 2021Quote: ... Cells reconstituted with pLenti CMVie-IRES-BlastR-based constructs were maintained with addition of 5 µg/ml and 1 µg/ml blasticidin (InvivoGen, Cat. no: ant-bl-05) for HEK293T and iBMDMs ...
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bioRxiv - Cancer Biology 2022Quote: ... Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen, San Diego, USA, #ant-pr-1).
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bioRxiv - Immunology 2021Quote: Human PBMC or purified primary cDCs were cultured in RPMI 1640 media supplemented with 10% Fetal Bovine Serum (HyClone) alone or in the presence of either 1μg/ml 2’3’-c’diAM(PS)2 (Invivogen) or 5μg/ml Poly I:C (SIGMA ...
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bioRxiv - Microbiology 2020Quote: ... adjuvanted with 500 μg aluminium hydroxide (Alhydrogel adjuvant 2%, vac-alu-250, InvivoGen, San Diego, CA, USA) in 100 μl volume on days 0 and 28 ...
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bioRxiv - Microbiology 2021Quote: ... by limiting-dilution and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen) for 3-5 d at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... by limiting-dilution and allowed to expand in growth medium supplemented with 2 μg/ml puromycin (InvivoGen) and 8 μg/ml blasticidin (InvivoGen ...
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bioRxiv - Immunology 2022Quote: ... HEL2XOVApep or HEL3XOVApep adsorbed on 45 or 90 µg alum adjuvant (Alhydrogel, InvivoGen cat. 21645-51-2).
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bioRxiv - Immunology 2022Quote: SiO2 crystals free of bacterial contamination (Nano-SiO2, diameter less than 100 nm, InvivoGen, #tlrl-sio-2) at a concentration of 10 mg/ml supplemented with phenol red ...
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bioRxiv - Microbiology 2021Quote: Human IgA was purified from fecal supernatants with Peptide M/ Agarose (InvivoGen, Cat. No. gel-pdm-2) as described by the manufacturer ...