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Citations for Invivogen :
1201 - 1250 of 1284 citations for 6 chloro 3 hydroxymethyl 1 3 benzoxazol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and the protein was first adsorbed on 200 μg of aluminum hydroxide (alum; containing 100 μg of aluminum) before 20 μg of CpG1826 (vac-1826-1, Invivogen) were added at the point of injection ...
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bioRxiv - Immunology 2021Quote: 1×106 thawed PBMCs were incubated at 37 ⁰C and 5% CO2 during 18 hours in RPMI with 10% FBS without any stimuli or with 1 µM CpG-A (ODN 2216; InvivoGen). After incubation ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Cancer Biology 2022Quote: Mouse colonic organoids were maintained in maintained in advanced DMEM/F-12 supplemented with 1X Primocin (Invivogen #ant-pm-1), 10mM HEPES ...
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bioRxiv - Microbiology 2022Quote: VeroE6/TMPRSS2 (JCRB 1819) cells (Matsuyama et al., 2020) were propagated in the presence of 1 mg/ml geneticin (G418; Invivogen) and 5 μg/ml plasmocin prophylactic (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... and pKANA2CENPB followed by selection on hygromycin B (1 mg/mL, #089-06151, Wako) and blasticidin S (5 µg/mL, #ant-bl-05, InvivoGen).
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bioRxiv - Microbiology 2024Quote: ... Cells transduced with lentiviruses encoding for the different gRNAs were passaged in selection medium containing 10 µg/ml blasticidin and 5 µg/ml puromycin (ant-pr-1, InvivoGen) to obtain polyclonal cell populations lacking either GSDMD or GSDME ...
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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bioRxiv - Genomics 2023Quote: ... Cells were then imaged in the imaging buffer (0.5 μg/ml DAPI, 10 μg/ml Fungin (InvivoGen ant-fn-1) in PBS ...
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bioRxiv - Genetics 2024Quote: ... the pooled mixture of cells were expanded and subsequently maintained in D10-dox containing 1 μg/mL puromycin (InvivoGen, ANTPR).
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... OSCC cell lines were than infected with the lentiviruses in the supernatants and the pools were selected using 1 µg/ml puromycin (Invivogen) for 5 days ...
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bioRxiv - Immunology 2022Quote: ... with 5 µg H7 HA-12D adjuvanted with 150 µg alum alone or 150 µg alum and 1 µg CpG (ODN 1826, InvivoGen) via subcutaneous injection on days 0 and 21 ...
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bioRxiv - Neuroscience 2023Quote: ... The culture media was also supplemented with 0.2% (1:500 dilution from a 5 μg/ml working concentration) Plasmocin™ Prophylactic (ant. mpp; InvivoGen). The day of plating from cryopreservation was allocated as day 0 and 50% media changes were carried out every 2-3 days ...
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bioRxiv - Molecular Biology 2023Quote: ... were then stably integrated into this cell line by Cas9-mediated integration into the AAVS1 locus by simultaneous nucleofection of plasmids containing either targeting sgRNAS (AAVS1-T2 and AAVS1-T2, Table S3) and spCas988 and selected using 1 µg/mL Puromycin (Invivogen) to generate stable integrants ...
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bioRxiv - Molecular Biology 2023Quote: ... Jurkat cells expressing the TCR reporter construct were maintained in culture with 10 µg/ml blasticidin (Invivogen ant-bl-1). For TCR expression ...
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Genomics 2022Quote: ... while material that was collected with washing of the cell strainer surface with ice cold PBS with 1% (v/v) primocin (InvivoGen) was referred to as jejunal epithelial cell fraction two (IEC-2) ...
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bioRxiv - Immunology 2023Quote: ... Cat# tlrl-hprna), LyoVec (Cat# lyec-1) and dispersible whole glucan particles (WGP, Cat# tlrl-wgp) were purchased from InvivoGen. Recombinant murine IFN-β1 (Cat# 581304 ...
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bioRxiv - Immunology 2023Quote: ... wild-type BALB/c female mice were sensitized intraperitoneally twice at 7-day intervals with either PBS (Group A) or 40 μg of OVA (Group B-F) together with 1 mg of aluminum hydroxide adjuvant (InvivoGen) on day 0 and day 7 ...
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Pathology 2023Quote: ... The present study used the MTA model using 1 µg/ml unmethylated CpG oligonucleotide (CpG ODN: InvivoGen, San Diego, CA) or P ...
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bioRxiv - Bioengineering 2024Quote: ... K562s were transfected according to manufacturer’s protocol with EGFR using pre-packaged lentiviral particles (G&P Biosciences) and selected for EGFR expression by culture in 1 µg/mL puromycin (InVivoGen). K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences ...
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bioRxiv - Genetics 2024Quote: ... and nitrate salts are as described in the appendix of reference (Kafer E, 1977) supplemented with 1% sorbitol and 20 mg/liter phleomycin (InvivoGen) after adjusting the pH to 7.0 ...
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bioRxiv - Immunology 2021Quote: ... BMDCs were surface-labeled with fluorochrome-coupled antibodies to the following antigens (all 1:400): MHCII-v450 (Invivogen, 48-5321-82), CD11b-PerCP-Cy5.5 (Invivogen ...
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
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bioRxiv - Neuroscience 2021Quote: ... or the same amount of IgG negative control antibody (Bio-Rad, MCA6004GA for MARCO and Invivogen, bgal- mab10-1 for TLR2) was administered in the same needle with IL-33.
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bioRxiv - Cell Biology 2021Quote: ... or photoactivatable GFP-α-tubulin (plasmid provided by Alexey Khodjakov) were cultured in DMEM supplemented with 1 mg/mL G418 (InvivoGen). All cell lines were grown in medium supplemented with 10% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Pathology 2020Quote: ... reporter gene under the control of an IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites (Invivogen).
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bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
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bioRxiv - Immunology 2022Quote: ... were generated by transfecting OKT3-2A-Hygromycin_pEK plasmid as previously described and selected for with 0.4mg/mL hygromycin (InvivoGen, ant-hg-1) (12) ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Omicron Variant (B.1.1.529/BA.1) pLV-SpikeV11) and Omicron Variants (BA.4/BA.5) pLV-SpikeV13) were purchased from InvivoGen (San Diego, CA). The human T-Cell lymphoma Jurkat (E6-1 ...
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bioRxiv - Bioengineering 2024Quote: ... Results were collected 24 hours after treatment using a mouse IFN-β solid-phase sandwich ELISA kit (Invivogen Cat#42400-1) on cell supernatants following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Results were collected 24 hours after treatment using a mouse IFN-β solid-phase sandwich ELISA kit (Invivogen Cat#42400-1) on cell supernatants following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... GFP only) were cultured in the same medium and selected by adding 100 μg/ml of hygromycin B (InvivoGen, ant-hg-1) to the media ...
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bioRxiv - Molecular Biology 2021Quote: ... Stable transformants were maintained in media supplemented with blasticidin and 50 μg/ml Hygromycin B Gold (InvivoGen, cat n°ant-hg-1).
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bioRxiv - Molecular Biology 2019Quote: ... Then digested tissues were triturated with a pipette in plating medium (DMEM with 10% FBS and 100 μg/mL antimicrobial agent Primocin™ (ant-pm-1, Invivogen, USA). Dissociated neurons were immediately plated onto 35 mm dishes coated with 50 μg/mL poly-D-lysine (P6407 ...
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bioRxiv - Immunology 2021Quote: ... about 0.5cm² and incubated at 4C in an antibiotic mixture consisting of 1X PBS +100ug/mL Normocin (Invivogen Cat# ant-nr-1), 50ug/mL Gentamicin (Amresco ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
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bioRxiv - Immunology 2022Quote: ... stimuli were as follows: 1×108 pfu/well of purified phage preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). The TLR3 signalling inhibitor (EMD Millipore ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the library were measured by mCherry flow cytometry and selected with 1 μg/ml puromycin (Invivogen, ant-pr). The selected cells were expended in culture for 13-14 doublings to obtain enough number of cells for xenograft experiments.
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bioRxiv - Immunology 2022Quote: A single dose consisted of 10 μg of peptide (peptide stock is 10 mg/mL resuspended in DMSO) was added to 25 μg cyclic-di-GMP (stock is 1 mg/mL resuspended in PBS) (Invivogen tlrl-nacdg) and PBS added to a final volume of 50 μL ...