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Citations for Invivogen :
1151 - 1200 of 1314 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were routinely checked to be free of mycoplasma contamination using Plasmo Test Mycoplasma Detection Kit (InvivoGen, rep-pt1).
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bioRxiv - Immunology 2021Quote: Enriched human memory B cells were cultured at 0.2 x 106 cells per ml in complete RPMI supplemented with 1 mg/ml R848 (Invivogen), 1 mg/ml soluble CD40L (Fitzgerald) ...
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bioRxiv - Genomics 2020Quote: ... These 4T1 cells were always kept in selection medium containing 10 µg/mL of blasticidin (ant-bl-05, Invivogen). When reaching 70% confluency ...
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bioRxiv - Immunology 2021Quote: ... For rescue assays cells were treated at the time of LPS priming with 10 μM AC-YVAD-CMK (Invivogen), 1 mM GNE9605 (Cayman Chemicals) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as a measure of TLR4 activation in HEK-null2 and HEK-hTLR4 cells as recommended by the provider (Invivogen) Secreted cytokines were collected 48 hrs post-agonist treatment and quantified by ELISA following the manufacturer protocols ...
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bioRxiv - Microbiology 2023Quote: ... cells were placed under drug selection in 10% FBS/RPMI containing 1 µg/ml puromycin (InvivoGen, Cat# ant-pr) or 6 ng/ml blasticidin (InvivoGen ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte THP1 cells (TIB2002, ATCC) were differentiated in macrophages with phorbol-12 myristate-13-acetate (PMA) (#tlrl-pma, Invivogen), as previously described (80 ...
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bioRxiv - Immunology 2023Quote: ... ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37°C for 24 hours before supernatants were collected and mixed with QuantiBlue reagent (Invivogen). Colour changes ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were serially diluted in growth medium and added to the washed plate with 100,000 Jurkat Lucia NFAT CD16 cells/well (Invivogen). Antibodies and cells were incubated for 24 hours at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 5000 B cells were then cultured in the presence of 50,000 sorted B220-cells and with 1ug/mL R848 (tlrl-r848, InVivoGen) and anti-CD40 (clone FGK4.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Stable populations of puromycin-resistant cells were obtained by selecting in 1ug/mL puromycin (Invivogen, San Diego, CA, USA) for at least 48hrs.
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bioRxiv - Cell Biology 2023Quote: ... the first seven days in HAM F12K Complete containing antimicrobial agent for primary cells (Primocin, cat. ant-pm2, InvivoGen) (1:500 ...
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bioRxiv - Immunology 2023Quote: The presence of TLR’s agonists in circulation was determined by exposing the Raw Blue macrophage reporter cell line (InvivoGen) to mice sera ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Cells were stimulated as follows: (1) stimulated with 1 μg/ml high-molecular mass poly(I:C) (Invivogen, tlrl-pic) transfected with 2 μg/ml Lipofectamin 2,000 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... or HEK-Dual RNA 4KO MDA5 cells were cultured overnight with increasing concentrations of Poly(I:C) HMW (Cat. #.: tlrl-pic, InvivoGen), Poly(I:C ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants from THP-1 Dual cells (20 µl) were mixed with QUANTI-Luc 4 Lucia/Gaussia reagent (Invivogen), and luminescence was measured by a luminometer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were selected with 2.5 µg/ml (PC3) and 5 µg/m (DU145) of blasticidin (InvivoGen ant-bl-1) for 7-9 days ...
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bioRxiv - Immunology 2023Quote: ... FO B cells were sorted (the gating strategy is shown in Figure S4A) and stimulated with 2.5 µM CpG (InvivoGen) and nicotine (Sigma ...
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... The cells were maintained in RPMI-1640 containing 10% FBS and 1 µg/mL puromycin (InvivoGen, ant-pr-1). The THP-1 human monocytic cell line was purchased from ATCC and maintained in RPMI-1640 supplemented with 10% FBS and 55 µM beta-mercaptoethanol at 37°C with 5% CO2.
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bioRxiv - Biochemistry 2023Quote: HKB11 PD-1 cells were used as stable pool generated by transfecting HKB11 cells with pMAX vector41 encoding human PD-1 (UniProt Q15116) and selection with 0.8 mg/mL geneticin (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... cells were trypsinized and seeded into 100 mm dishes and selection was initiated with 10 μg/ml blasticidin (Invivogen) and 200 μg/ml hygromycin B (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
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bioRxiv - Genomics 2024Quote: ... THP1-ASC-GFP-Cas9 CASP1/8KO Brunello-iT7 cells were pre-differentiated overnight with 100 ng/ml PMA (Invivogen). The next day ...
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bioRxiv - Developmental Biology 2024Quote: All cells were cultured in DMEM/F12 containing 10% FBS and Primocin (50 ng/ml) (InvivoGen, ant-pm-1). DPP4+ ...
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bioRxiv - Microbiology 2024Quote: ... NF-kB activation was measured at OD650 after incubation of cell supernatants in QUANTI-BlueTM medium as described in manufacturer’s instructions (InvivoGen).
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bioRxiv - Microbiology 2024Quote: ... and diluted to 2.5 x 105 cells/ml in complete RPMI with 100 nM phorbol 12-myristate 13-acetate (PMA) (InvivoGen) to differentiate cells to a macrophage phenotype ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were tested for mycoplasma contamination once every two months using the Mycotest kit (InvivoGen, rep-mys-10).
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bioRxiv - Immunology 2021Quote: The transduced THP-1 cells were differentiated with 200 nM PMA for 24 h and treated with DOX (Wako) or nigericin (InvivoGen) at the indicated concentrations ...
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bioRxiv - Immunology 2021Quote: ... For control purposes cells were stimulated with cytosolic 1 µg LPS using lipofectamine or nigericin (6.7 µM) (InvivoGen, tlrl-nig). 1% triton X100 was used as a lysis control in cell death assays (37) ...
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Neuroscience 2020Quote: Stably transfected HEK293 Flp-In T-Rex cells were grown and maintained in DMEM supplemented with 10% FBS and 100 µg/mL Hygromycin (InvivoGen) and 15 µg/mL Blasticidin (InvivoGen) ...
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The stably transduced population of cells was selected using appropriate antibiotics (5 ug/ml puromycin or 50-100 μg/ml hygromycin Gold, both from Invivogen) for at least 5 days ...
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bioRxiv - Microbiology 2020Quote: ... supernatants from iBMDM infections were incubated with the reporter cell line and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per manufacturer’s instructions using recombinant mouse IFNβ (PBL assay science ...
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bioRxiv - Cell Biology 2021Quote: ... Stable expression of the constructs in the HEK 293 cells was maintained by selection with hygromycin B (250 μg/ml final; Invivogen). For experiments ...
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bioRxiv - Immunology 2020Quote: Neutrophils isolated from 4 healthy volunteers were plated at 50,000 cells per well and stimulated in duplicate with the following ligands for 1 hour: lipotechoic acid (LTA) 100ng/mL (Invivogen), LPS (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... the medium was further supplemented with puromycin (7 μg/ml for selection of clones or 6 μg/ml for maintenance of cell lines; Invivogen) and/or hygromycin (100 μg/ml for selection of clones or 70 μg/ml for maintenance of clones ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK-293T cells used for these studies have been authenticated by STR profiling and mycoplasma testing is done quarterly using PlasmoTest (Invivogen).
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bioRxiv - Cancer Biology 2022Quote: ... The cell lines were maintained and Mycoplasma absence was tested regularly using the PlasmoTest kit (HEK blue, Invivogen, Toulouse, France). HCT116 NCLX KO cells are the same cells described in Pathak et al. ...
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bioRxiv - Immunology 2022Quote: ... Sorted cells were seeded at a final density of 1 x 106 cells/mL and were left unstimulated or stimulated with 100 ng/mL LPS (Escherichia coli O111:B4; Invivogen) or 1000 U/mL IFN-β (PBL Interferon Source ...
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bioRxiv - Cell Biology 2022Quote: ... by stimulating cells with a range of concentrations (25 nM – 200 nM) of phorbol 12-myristate 13-acetate (PMA; InvivoGen) for different incubation periods ...
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bioRxiv - Immunology 2020Quote: ... adherent cells were replated, and stimulated with either LPS (100ng/mL, Enzo) or whole glucan particles (100 μg/mL, Invivogen).
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bioRxiv - Microbiology 2020Quote: ... RAW Blue cell-free supernatants were collected and 50 μl was mixed with 150 μl of the QUANTI-Blue substrate (InvivoGen) and incubated at 37° C for 45 minutes in a flat bottom 96-well plate ...
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bioRxiv - Microbiology 2020Quote: ... The transduction media was then changed with fresh DMEM for 24 hours then the transduced cells were selected using DMEM containing 10 μg/mL Blasticidin (Invivogen) and 1 μg/mL Puromycin (Invivogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed in 1x phosphate buffer saline (PBS) and subsequently incubated with puromycin containing media (InvivoGen, 10 μg/ml) for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: Cultured cells were collected in a pre-cooled ep-tube using a cell scraper (SPL) and lysed in ice-cold RIPA buffer (InvivoGen) containing protease and phosphatase inhibitors ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1×106 T cells per well in RPMI medium supplemented with Primocin (100 µg/ml; Invivogen), 10 % charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2 and A549-S cell lines were selected using 10 μg/ml puromycin (InvivoGen, catalog no. ant-pr-1) 4 days post-transduction ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines have been routinely tested for mycoplasma contamination (Mycoplasma contamination detection kit; rep-pt1; InvivoGen - San Diego - CA).