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Citations for Invivogen :
1101 - 1150 of 1152 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the library were measured by mCherry flow cytometry and selected with 1 μg/ml puromycin (Invivogen, ant-pr). The selected cells were expended in culture for 13-14 doublings to obtain enough number of cells for xenograft experiments.
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bioRxiv - Immunology 2022Quote: A single dose consisted of 10 μg of peptide (peptide stock is 10 mg/mL resuspended in DMSO) was added to 25 μg cyclic-di-GMP (stock is 1 mg/mL resuspended in PBS) (Invivogen tlrl-nacdg) and PBS added to a final volume of 50 μL ...
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bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
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bioRxiv - Bioengineering 2023Quote: ... the mice were intratumorally injected with 40 μg of STINGΔTM protein mixed with 1 μg of 2’3’-cGAMP (InvivoGen, Cat # tlrl-nacga23-02) in 25 μL sterile PBS ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
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bioRxiv - Biochemistry 2024Quote: ... CD8+ T cell depletion was done for one treatment group (targeted HC-HAdV5HVR7, encoding for li-OVA.1+2 + muIL2v) with 0.2 mg mCD8-mIgG2 (InvivoGen 10354-42-01) via intraperitoneal injection per mouse and application ...
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bioRxiv - Neuroscience 2024Quote: ... which was composed of DMEM (Thermo, 41966) containing 10% Heat-inactivated FBS (HI-FBS, Cytiva, SH30071.03HI) and 2% primocin (Invivogen, ant-pm-1). The next day ...
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bioRxiv - Cell Biology 2024Quote: ... Neutrophils were resuspended at 1×107 cells/mL in complete media w/o phenol red supplemented with 20 ng/ml GM-CSF and pulsed with 1 μg/ml LPS (InvivoGen, # tlrl-smlps) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were infected with virus in the presence of 5 µM polybrene for two days and subsequently selected either through antibiotic selection with 1 µg/ml puromycin (InvivoGen, ant-pr), 10 µg/ml blasticidin (InvivoGen ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
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bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
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bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Developmental Biology 2022Quote: ... dTomato-positive cells were sorted on a BD FACS Aria III using BD FACSDiva 8.0.1 Software using a 100 µm nozzle and cultured on Martigel-coated plates in mTeSR Plus supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and 25 µm Y-27632 ...
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bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Physiology 2021Quote: ... Duodenal or jejunal segments (∼6 cm) were isolated and placed into falcon tubes containing cold DMEM (high glucose, 4.5 g/l) containing Primocin (Invivogen, cat# ant-pm-1). Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the targeting vectors and incubated for 2 days before addition of selective medium containing 1.5 μg/ml puromycin (Invivogen, cat# ant-pr-1). After 5 days ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated on glass-teflon microscope slide (Tekdon, #518plain) and the next day treated with either DMSO or Phleomycin (InvivoGen # ant-ph-1) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2023Quote: ... We used a variety of inhibitors including Torin 1 (inh-tor1) and Bafilomycin A1 (tlrl-baf1) both purchased from InvivoGen (San Diego, CA). INK128 (S2811) ...
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bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 1 mg/kg of lipopolysaccharide (LPS endotoxin from E. coli 0111:B4 ultra-pure; InvivoGen, San Diego, CA) dissolved in saline ...
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bioRxiv - Cell Biology 2024Quote: ... was a gift from Kristen Brennard and stably expressed in RPE1 cells using puromycin selection (10 ug/mL, Invivogen Cat#ant-pr-1). CA-YAP and dCas9-expressing cells were generated using third-generation lentiviral transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... T47D and MCF7 cells were transduced with the lentiviral particles and stably expressing cells were generated after selection using G418 (InvivoGen ant-gn-1) for two weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... 293T cells transduced to stably express TIM1 (TIM1-293T) or mock transduced (Mock-293T) were maintained in the medium supplemented with 1 μg/mL puromycin (InvivoGen, Cat# ant-pr).
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bioRxiv - Biochemistry 2024Quote: ... cells were covered with selection media comprised of DMEM + 10 % HI FBS + 1% PS and 2 μg/mL puromycin (Invivogen 58-58-2). Selection media was replaced every 48 h until control WT cells were completely dead ...
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bioRxiv - Cell Biology 2024Quote: ... Stable cell lines generated in this background were cultured with 5 µg/ml of blasticidin and 200 µg/ml of hygromycin B (Invivogen #ant-hg-1). To minimise background expression of the transgene ...
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bioRxiv - Cell Biology 2024Quote: ... For GSK3 knockout/knockdown cells were generated by transfecting 293T cells with the corresponding lentiCRISPR v2 plasmid with jetPRIME (Polyplus-transfection, Cat# 101000046) and selected with puromycin (InvivoGen ant-pr-1) (1 μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in complete RPMI-1640 medium and were transfected with 1 mg/ml cGAS agonist (G3-ended Y-form Short DNA) (InvivoGen, San Diego, USA) and LyoVec™ (InvivoGen ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Immunology 2021Quote: ... NLRP3-/- iBMDMs or NLRP3-/- iBMDMs reconstituted with mScarlet-tagged NLRP3 were seeded at 0.5×106 cells/well on a 12-well tissue culture plate and on the next day treated or not with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... Transgenic Col-0 (LUC) plants were selected on 1/2 MS medium supplemented with 50 μM hygromycin B (InvivoGen, Cat No. ant-hg-5). LUC activity was confirmed as described above ...
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated for 24 hours with 1×108 pfu/well of purified Pf4 phage or equivalent volume ΔPf4 preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). Cells were centrifuged at 300 x g for 5 minutes and supernatant was collected and used for cytokine profiling through Luminex (The Human Immune Monitoring Center ...
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bioRxiv - Developmental Biology 2024Quote: ... The EGFP-fused lineage barcode was confirmed to exist as a single copy in the genome and to be highly expressed after blasticidin selection (15 g/ml, InvivoGen, no. ant-bl-1) and flow cytometry sorting ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Immunology 2021Quote: ... Cells reconstituted with pLenti CMVie-IRES-BlastR-based constructs were maintained with addition of 5 µg/ml and 1 µg/ml blasticidin (InvivoGen, Cat. no: ant-bl-05) for HEK293T and iBMDMs ...
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bioRxiv - Cancer Biology 2022Quote: ... Stably transduced cells were either selected by flow cytometry using BD FACSAria III cell sorter or 1μg/ml puromycin (InvivoGen, San Diego, USA, #ant-pr-1).