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Citations for Invivogen :
1001 - 1045 of 1045 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... 20 μl of supernatants from the HEK-Blue™ or THP1-Blue™ cells were mixed with 180 μl of QuantiBlue reagent (InvivoGen) and optical density (O.D. ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Bioengineering 2020Quote: ... 20 µL of cell culture supernatant was then withdrawn and analyzed for alkaline phosphatase content via change in Quanti-Blue (Invivogen, rep-qbs) absorbance at 620 nm (Spectramax id3 plate reader).
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were treated with 400 µM FFA and 3 µM HSG4112 in the presence or absence of bafilomycin A1 (Invivogen, tlrl-baf1). Cellular localization of LC3B was observed using a Carl Zeiss Confocal LSM710 Meta microscope and the images were processed with the software supplied by the manufacturer (Carl Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... During this incubation cells were resuspended in DMEM supplemented with 10% (vol/vol) FBS and 1x Normocin (InvivoGen, San Diego, CA, USA) and 6,000 cells were dispensed onto each well loaded with medium for a final siRNA concentration of 20 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... ≈80% confluent adherent cells were washed twice in 1× PBS and subsequently pulsed with puromycin-containing media (InvivoGen, 10 μg mL−1) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... A549-Cas9 cells were transduced with a lentivirus containing hACE2 and subjected to Zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA) at 72 h after transduction ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Neuroscience 2023Quote: ... To assess autophagic function, cells were treated with Bafilomycin A1 (BafA1, 200 nM, Enzo, BML-CM110-0100) and/or Torin1 (200 nM, Invivogen, inh-tor1). All compounds were mixed with culture medium and placed onto plated cells for 4 h before extraction.
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bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
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bioRxiv - Bioengineering 2024Quote: All materials for in vivo use were tested for endotoxin using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
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bioRxiv - Neuroscience 2019Quote: ... Tet-inducible EGFP-RBM45 stable cell lines (see below) were maintained in the presence of 50μg/ml Zeocin (Invivogen, San Diego, CA, USA). The expression of EGFP-RBM45 was induced with 1 μg/ml doxycycline hyclate (Sigma ...
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bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were selected by at least one-week treatment with 150 ug/ml G418 or 10 μg/mL blasticidin (InvivoGen, San Diego, CA). Bulk populations of selected cells were used in this study ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Immunology 2020Quote: ... Immune cell stimulations were performed in the absence (Control) or presence of 0,1 μg/ml of extrapure Lipopolysacharide (Invivogen LPS, Cat. tlrl-3pelps), 10 μg/ml Poly I:C (Invivogen ...
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bioRxiv - Cancer Biology 2022Quote: ... The final product was verified with Sanger sequencing from both directions and incorporated into cells using PEI transfection and selection with G418 (InvivoGen, ant-gn-5).
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL of supernatants from the reporter cells (HEK-Blue IFN-α/β or HEK-Blue IFN-λ) were added to 180 μL of Quanti-blue substrate (InvivoGen) and incubated at 37°C for 1-2 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were routinely confirmed to be negative for mycoplasma infection every 3–4 months using PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Cas9+mCherry+ RMS cells were then transiently transfected with pLentiguide-PURO plasmid and selected with 1 µg/ml Puromycin (InVivoGen, #ant-pr-1) for two days ...
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bioRxiv - Immunology 2023Quote: The ADCC potential of individual monoclonal antibodies was measured as described (25) using a Jurkat Lucia NFAT cell line (Invivogen, jktl-nfat-cd16), cultured according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... cells were washed in 1x phosphate buffer saline (PBS) and subsequently incubated with puromycin containing media (InvivoGen, San Diego, California, USA, 10 µg/ml) for 10 min ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Molecular Biology 2021Quote: ... HuH-7 Flp-In cell line stocks and their derivatives were cultured in medium containing zeocin (100 µg/ml, InvivoGen, Cat#: ant-zn-05) or hygromycin B Gold (200 µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... The stably transfected cells carrying the Zeocin antibiotic resistance gene (Sh-ble) were selected with and maintained in Zeocin (800 mg/ml) (Invivogen, San Diego, CA, USA). Following 10-15 days of incubation in the presence of Zeocin ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... After 48 h cells were put under antibiotic selection using 400 μg/ml zeocin and 10 μg/ml Blasticidin S (Invivogen, San Diego, California, US)) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20μl of HEK cell supernatant was transferred to a flat-bottom 96-well plate containing 180 μl of QuantiBlue (Invivogen, cat. code rep-qbs) detection reagent and incubated at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... cells were transduced similarly with supernatant containing ACE2-encoding lentivirus and selected with 1 ug/mL of puromycin (InvivoGen, San Diego, CA, catalogue #ant-pr-1).
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bioRxiv - Bioengineering 2023Quote: ... 4 or 8 µg/ml) or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...