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Citations for Invivogen :
951 - 1000 of 1045 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... while material that was collected with washing of the cell strainer surface with ice cold PBS with 1% (v/v) primocin (InvivoGen) was referred to as jejunal epithelial cell fraction two (IEC-2) ...
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bioRxiv - Immunology 2023Quote: ... Ultrapure LPS for cell culture experiments (lipopolysaccharide from Escherichia coli 0111:B4) was purchased from Invivogen (San Diego, CA, USA).
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bioRxiv - Immunology 2023Quote: Bioactive functional type I IFN was quantified in supernatants using the reporter cell line HEK-Blue IFN-α/β (Invivogen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
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bioRxiv - Immunology 2023Quote: ... cells were pretreated with the caspase-1 inhibitor YVAD (Enzo) (1 μM) or with the NLRP3 inhibitor MCC-950 (Invivogen) (10 μM ...
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bioRxiv - Immunology 2024Quote: ... with WT or Sulf2+/- antigen-presenting cells (BMDM or DCs) activated with LPS (100 ng/ml LPS, 4 h, InvivoGen). As a source of antigen ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 ITGB1 KO cells were transduced with pWPI-ITGB1 and selected in cDMEM supplemented with 400 μg/ml G418 (Invivogen). S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL; Invivogen, ANT-PR-1). After six days of selection ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were expanded to a surface-treated T-75 flask and treated with 2 µg/mL of puromycin (Invivogen) for 2 weeks for selection of the transduced cells to generate the cell line expressing hM3D(Gq) ...
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bioRxiv - Immunology 2024Quote: ... 2cz-Tag-it co-culture with RAW 264.7 cells activated for 6 hours with 1.5 ug/ml Ultrapure LPS from E.coli O111:B4 (InvivoGen, Cat#tlrl-3pepls) and 2cz cultured with 50 ng/ml of its strong agonist peptide SIYRYYGL (GenScript ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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bioRxiv - Cancer Biology 2024Quote: ... 24-well or 96-well plates at a concentration of 5×105 cells/mL in RPMI 1640 supplemented with 20% fetal calf serum and 1X primocin (Invivogen).
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bioRxiv - Genomics 2023Quote: ... Cells were then imaged in the imaging buffer (0.5 μg/ml DAPI, 10 μg/ml Fungin (InvivoGen ant-fn-1) in PBS ...
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bioRxiv - Genetics 2024Quote: ... the pooled mixture of cells were expanded and subsequently maintained in D10-dox containing 1 μg/mL puromycin (InvivoGen, ANTPR).
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bioRxiv - Biochemistry 2024Quote: ... The identities of cell lines were confirmed by chromosomal marker analysis and tested negative for mycoplasma using a MycoStrip (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... HEK-Blue TLR3 and HEK-Blue TLR4 reporter cells (Cat. no.# hkb-htlr2, hkb-htlr3 and hkb-htlr4) were purchased from InvivoGen (USA). Human monocytic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of the supernatant or an IFNα/β standard containing purified IFNα and IFNβ were added to 180 μL HEK-Blue cells (Invivogen) (50,000 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/uL primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in E14 standard medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... The clonal Dia-C6 cells were used for lenti-sgRNA transduction followed by 20 µg/mL blasticidin (InvivoGen, ant-bl-1) selection and fluorescence cell sorting for mNeonGreen or mScarlet (see Plasmids ...
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bioRxiv - Immunology 2019Quote: ... cells were incubated for additional 5h with 50 ng/ml Phorbol-12- myristate-13-acetate (PMA) (InvivoGen, San Diego, CA, USA) + 1 µg/ml Ionomycin (MP Biomedicals ...
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bioRxiv - Physiology 2021Quote: ... transfection: Cells with 60-80% confluency were transfected with synthetic double-stranded RNA poly(I:C) (high molecular weight, InvivoGen #tlrl-pic) using lipofectamine 2000 following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... A459/KZFP cells were cultured in F-12K medium with 1.0 μg/ml puromycin (Invivogen, San Diego, CA; #ant-pr-1). An A549 cell line stably expressing Cas9 (A549/Cas9 cells ...
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bioRxiv - Cell Biology 2022Quote: ... 24 and a Blasticidin resistance gene used for the initial selection of infected cells (10 µg/ml Blasticidin, Invivogen, ant- bl). MEF cells were cultivated in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Microbiology 2022Quote: ... RIG-I stimulation was assessed by analyzing Lucia luciferase reporter gene expression in 20 µl cell culture supernatants using QUANTI-Luc™ (Invivogen). Data are expressed as the normalized fold change over mock infected cells ...
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bioRxiv - Microbiology 2021Quote: ... 50 µL of each cell supernatant were transferred into separate plates and added 50 µL of quantiblue reagent™ following manufacturer’s instructions (InvivoGen Inc.). Afterwards ...
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bioRxiv - Systems Biology 2021Quote: ... Different innate immune stimuli were applied to cell medium at 10% culture volume as follows: High molecular weight (HMW) poly(I:C) (Invivogen #tlrl-pic) at a concentration of 1mg/mL for 2 h (final concentration in the well 100 ug/mL ...
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bioRxiv - Systems Biology 2021Quote: ... 20 μL of the cell media supernatant was added to 50 μL of QUANTI-Luc™ assay solution (Invivogen #rep-qlc1) in a white 96-well microplate (Greiner ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Cell Biology 2021Quote: ... Double knockout cells were selected by using a combination of 1μg/ml Puromycin and 6μg/ml Blasticidin (Invivogen, #ant-bl-10p) for 1 week.
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bioRxiv - Microbiology 2021Quote: ... HEK293-FT_hACE2 and A549_hACE2 cells were grown in their correspondent medium with 250-500 μg/ml of blasticidin (Invivogen # ant-bl-1). All cell media were supplemented with 8% (v/v ...
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bioRxiv - Immunology 2022Quote: ... THP-1 Dual cells were grown in the presence of respective selection agents [100 mg/mL zeocin (InvivoGen, #ant-zn-1) and 10 mg/mL blasticidin (InvivoGen ...
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bioRxiv - Molecular Biology 2022Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/µl primorcin (InvivoGen, ant-pm-1) and 10 µM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Microbiology 2022Quote: Selection of cells transfected with in vitro RNA transcripts produced from replicon constructs was initiated by addition of puromycin (InvivoGen, UK) to the culture media at 24 - 48 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... 20 µl of cell supernatant were transferred to new plates and supplemented with freshly made 180 µl Quanti-Blue solution (Invivogen, France). After 180 minutes of incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrigel/cell mix was incubated for 15 min at 37C to allow for solidification and base media with Primocin® (InvivoGen) was added to top and bottom chambers of transwell ...
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bioRxiv - Immunology 2023Quote: The RAW-Blue ISG macrophage cell line stably transfected with an ISGF3-dependent reporter construct was cultured and maintained according to the manufacturer’s instructions (RAW-Blue ISG cells, Invivogen raw-isg). ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: The inhibitory effect of APB-R3 on IL-18 signal transduction was tested using the HEK-BlueTM IL-18 reporter cell assay system (InvivoGen, US). APB-R3 and recombinant IL-18BP were appropriately diluted ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-ppp-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (Catalog Code, lyec-12, InvivoGen), following the manufacturer’ ss instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated for 6 h with the indicated concentration of either High Molecular Weight (HMW) Poly(I:C) (InvivoGen, tlrl-pic) or Poly(I:C ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were negative for mycoplasma contamination as confirmed using a MycoStrip™ - Mycoplasma Detection Kit (InvivoGen rep-mys-10). FBS was heat inactivated prior to use at 56°C for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: All materials were verified to be free of endotoxins using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) using the protocol provided by the supplier ...
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bioRxiv - Immunology 2024Quote: ... differentiated for seven days with 50ng/mL M-CSF) and adherent peritoneal exudate cells (PECs) incubated with and without 200ng/mL lipopolysaccharide (LPS, InvivoGen, #tlrlpb5lps) for 6 hours (Fig S1D) ...