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Citations for Invivogen :
51 - 100 of 170 citations for Recombinant Mouse TNFRSF17 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were incubated for 30 min at room temperature (RT) and 0.2 µg/ml of Fc-h-dectin-hFc (Invivogen) was added to the UV-irradiated conidia and incubated for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... and 10% FCS in a 96 well ‘U’ bottomed plate and stimulated with 1μg/mL LPS-EB Ultrapure (Invivogen) and Brefeldin A (eBioScience) ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Pam3CSK4 (100 µg/mouse; InvivoGen), or Poly(I:C ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2023Quote: ... B16-F10 cells expressing luciferase (B16-F10-luc) were generated by infection of recombinant lentivirus stably expressing luciferase luc2 under the control of hEF1A-eIF4G promoter (InvivoGen)61 ...
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bioRxiv - Immunology 2020Quote: ... clarified by centrifugation and subsequently incubated with Peptide M(Invivogen)/Protein G-coupled sepharose beads (Invivogen, catalog# gel-pdm-5 ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Bioengineering 2023Quote: ... 2×105 PBMCs and 4×104 autologous CD8+ T cells were incubated with either supernatants from engineered PCs or media containing recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) or anti-CD33 x anti-CD3 bispecific (AMG 330 ...
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bioRxiv - Bioengineering 2023Quote: ... 5×103 control cells and 5×104 CD8+ T cells were incubated with either various dilutions of supernatants from genome-engineered cells or media containing various concentrations of recombinant anti-CD19 x anti-CD3 bispecific (Invivogen, bimab-hcd19cd3) for 48 hours (Figure 1F-H & 3G-I) ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2020Quote: ... ovalbumin (OVA) EndoFit (5 μg/mouse) and Alhydrogel adjuvant 2% (alum, 100 μg/mouse) were purchased from Invivogen; recombinant hemagglutinin (rHA ...
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bioRxiv - Microbiology 2020Quote: ... with 50 μl/mouse of AddaVax (Invivogen) as the adjuvant ...
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bioRxiv - Immunology 2023Quote: ... or Poly(I:C) (100 µg/mouse; InvivoGen) in 200 µl PBS for 24 h ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 µg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 μg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Systems Biology 2020Quote: ... irrelevant protein Ovalbumin (OVA, Invivogen, San Diego, CA) and PD1 as an internal negative control were coupled to distinct fluorescent-barcoded MagPlex microspheres (Luminex Corporation) ...
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bioRxiv - Cancer Biology 2022Quote: ... Full-length ovalbumin protein (#vac-pova, InvivoGen, USA) was added at 100 μg mL-1 to the cells and incubated for 20 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... or the mouse kappa light chain plasmid (InVivoGen). The gBlock DNA and plasmids were cut with the specified enzymes ...
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bioRxiv - Bioengineering 2022Quote: ... Protein vaccines were administered in combination with Alhydrogel (Invivogen) at a 1:9 v/v ratio of adjuvant to antigen ...
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bioRxiv - Immunology 2022Quote: ... 5 μg HA protein combined with either Alum (InvivoGen) or AddaVax (InvivoGen ...
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bioRxiv - Immunology 2021Quote: ... Protein vaccines were administered with 1:10 AdjuPhos (Invivogen).
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bioRxiv - Microbiology 2023Quote: ... or 10 μg of protein adjuvanted with AddaVax (Invivogen)156,157 ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Immunology 2019Quote: ... a Mouse TLR Agonist kit was purchased from Invivogen and BMDMs were treated with each ligand for 16 hours ...
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bioRxiv - Cell Biology 2022Quote: ... ASC-expressing RAW264.7 mouse macrophages (RAW-ASC; InvivoGen raw-asc) are engineered to stably express murine ASC ...
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bioRxiv - Microbiology 2019Quote: ... Primary antibodies used: Mouse anti-HA antibody (InvivoGen, 1:1000), rabbit anti-ACP (1:2000) ...
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bioRxiv - Immunology 2021Quote: ... Mouse IFN-α was measured by a bioluminescence kit (InvivoGen). All assays were performed on cell free supernatants according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... or 200 µg of isotype control antibody (mouse IgG2a, Invivogen) three times at days 6 ...
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bioRxiv - Immunology 2023Quote: ... mice were immunised with VacOva (vac-pova, InvivoGen; 50μg/mouse) and LPS (L6529 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse STING activator DMAXX and cGAMP were purchased from InvivoGen.
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bioRxiv - Cancer Biology 2023Quote: ... Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14). Wild-type human NIK and the NIK-K429/430A mutant plasmids were a gift from Prof ...
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bioRxiv - Immunology 2020Quote: ... recipient mice were administered (i.p.) 100 μg EndoFit Ovalbumin protein (InvivoGen). After three days ...
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bioRxiv - Immunology 2020Quote: ... 5μg of PR8-HA protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink was injected into the left quadriceps and right gastrocnemius (50 μL per site) ...
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bioRxiv - Microbiology 2022Quote: A549-hACE2 lung carcinoma cells expressing the human ACE2 protein (Invivogen) were maintained in DMEM with 4.5 g/L glucose and 2 mM L-glutamine (Gibco) ...