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Citations for Invivogen :
51 - 100 of 214 citations for Recombinant Human ERBB2 protein Fc tagged R PE labeled since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...
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bioRxiv - Immunology 2023Quote: ... the human cDNA sequence (Invivogen) was subcloned into the pcDNA3 vector between the HindIII and EcoRV restriction sites followed by transfection of 5 µg plasmid into BAFF/CD40L double positive fibroblasts using Lipofectin reagent (Thermo) ...
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bioRxiv - Immunology 2022Quote: ... and human IL-1β (Invivogen), were dissolved in PBS and added to the cell culture medium to achieve final concentrations of 10 μM (ADP-heptose) ...
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bioRxiv - Immunology 2024Quote: ... 2) 0.2 ng/µL LPS-R (tlrl-eklps; Invivogen) as a positive control for human TLR4 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed twice with PBS and stained with Fc-hDectin-1a (Invivogen) and Calcofluor white (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: Engineered human THP1-Dual cells (Invivogen), derived from the human THP1 monocyte cell line ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...
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bioRxiv - Biophysics 2020Quote: Co-expression of FLAG- and HA-tagged UMOD isoforms was performed using a bicistronic vector pVITRO-hygro-mcs (Invivogen). For this purpose ...
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bioRxiv - Microbiology 2020Quote: ... Human CD14+ monocytes (2×106/ml) were pre-incubated with human anti-Dectin-1 (10μg/ml, Invivogen), anti-TLR2 blocking antibodies (10μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells without puromycin resistance were labeled with 10ug/ml Puromycin (Invivogen, ant-pr) for 10min ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Immunology 2022Quote: cDNA encoding a truncated human ACE2 fused to human albumin was sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vector was transiently transfected into Expi293F cells in suspension (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... A549 lung carcinoma cells expressing human ACE2 and human TMPRSS2 (A549.ACE2+.TMPRSS2+; cat n° a549-hace2tpsa, Invivogen) were grown in DMEM/10% FBS supplemented with 100 µg/ml Normocin ...
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bioRxiv - Cell Biology 2020Quote: ... 10% R-spondin (conditioned medium) and 100 μg/ml Primocin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... In some assays human anti-RBD (Invivogen) of known antibody concentration was used as standard ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Immunology 2021Quote: Anti-human CD20 murine IgG2a (hcd20-mab10, InvivoGen) and mouse anti-Biotin mIgG2a [3E6] (ab36406 ...
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bioRxiv - Cell Biology 2021Quote: ... Human TMPRS2 (pUNO1-hTMPRSS2a) was ordered from InvivoGen. Full length SARS-CoV-2 Spike plasmid (VG40589-UT ...
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bioRxiv - Immunology 2023Quote: HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
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bioRxiv - Microbiology 2020Quote: ... or OVA protein (InvivoGen) was administered iv to the infected mice.
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bioRxiv - Microbiology 2020Quote: ... or OVA protein (InvivoGen) adjuvanted with 1 μg of monophosphoryl lipid A (MPLA ...
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bioRxiv - Microbiology 2024Quote: ... or OVA protein (InvivoGen) was administered intravenously (iv. ...
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bioRxiv - Microbiology 2024Quote: ... or OVA protein (InvivoGen) adjuvanted with 1 µg of monophosphoryl lipid A (MPLA ...
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bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
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bioRxiv - Microbiology 2023Quote: ... Samples were incubated for 30 min at room temperature (RT) and 0.2 µg/ml of Fc-h-dectin-hFc (Invivogen) was added to the UV-irradiated conidia and incubated for 1 h at RT ...
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bioRxiv - Immunology 2023Quote: ... and 10% FCS in a 96 well ‘U’ bottomed plate and stimulated with 1μg/mL LPS-EB Ultrapure (Invivogen) and Brefeldin A (eBioScience) ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were gel purified and cloned into heavy and kappa expression plasmids52,53 adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 ampicillin resistant vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... Single chain human IL-12 was purchased from Invivogen. Blinatumomab (anti-hCD19-CD3 ...
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bioRxiv - Microbiology 2024Quote: ... human carcinoma epithelial lung A549 (Invivogen Inc, Toulouse, France), human microglial clone 3 HCM3 (ATCC ...
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bioRxiv - Microbiology 2024Quote: ... human carcinoma epithelial lung A549 (Invivogen Inc, Toulouse, France), and Monkey kidney normal Vero E6 (CCL-81 ...
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bioRxiv - Microbiology 2023Quote: Human HepG2 liver carcinoma AhR-Lucia reporter cells (InvivoGen) were cultured according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Labeled cells (0.5x106 cells/mL) were cultured for 2-3 days with 5 μg/mL R848 (InvivoGen), 50 U/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2020Quote: Biological activity of laminarin in LamOVA conjugates was analyzed by ELISA using a soluble murine Fc-Dectin-1a receptor (InvivoGen). Hypoallergenicity was analyzed in vitro as a measure of rat basophil leukemia cell (RBL-2H3 ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells stably expressing GFP were cultured in RPMI 1640 containing 10% (v/v) FCS and 5 μg/ml blasticidin (InvivoGen). Mouse thymocytes were cultured in RPMI1640 medium containing 10% (v/v ...
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bioRxiv - Immunology 2021Quote: ... reagents in human TLR1-9 Agonist kit (InvivoGen, tlrl-kit1hw) were diluted to working concentration in PBS ...
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bioRxiv - Microbiology 2024Quote: ... HEK293 cells expressing human ACE2 and TMPRSS2 (Invivogen; hkb-hace2tpsa) were propagated in complete DMEM supplemented with puromycin (0.5mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... B16-F10 cells expressing luciferase (B16-F10-luc) were generated by infection of recombinant lentivirus stably expressing luciferase luc2 under the control of hEF1A-eIF4G promoter (InvivoGen)61 ...
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bioRxiv - Immunology 2024Quote: ... The macrophages were then polarized to M1 by first adding 20 ng/mL of recombinant IFNγ for 24h and then 100 ng/mL of LPS (Invivogen) for another 24h ...
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bioRxiv - Immunology 2020Quote: ... Human THP-1 monocyte-like cells (THP-1 Lucia ISG, Invivogen) were maintained in RPMI 1640(Thermo Fisher cat ...
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bioRxiv - Immunology 2023Quote: ... IgG3 concentrations were measured by an IgG3 Human ELISA Kit (Invivogen).
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bioRxiv - Immunology 2023Quote: Human monocytes were stimulated with 10ug/mL TL8 (Invivogen, tlrl-tl8506), 10ug/mL ssPolyU(Invivogen ...
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bioRxiv - Cell Biology 2024Quote: ... human lung adenocarcinoma epithelial A549-Dual cells (InvivoGen, catalog #: a549-nfis) and human embryonic kidney HEK293T (ATCC ...