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Citations for Invivogen :
51 - 100 of 126 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 800ng-1μg of RNA was reverse transcribed in a mix containing M-MLV reverse transcriptase (Invivogen), dNTP mix (Biobasic) ...
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bioRxiv - Immunology 2021Quote: ... reagents in human TLR1-9 Agonist kit (InvivoGen, tlrl-kit1hw) were diluted to working concentration in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Immunology 2023Quote: ... Serum flow-through was then passed through 1 mL of Peptide M agarose (InvivoGen, gel-pdm-5) affinity column in gravity mode ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2020Quote: ... Human THP-1 monocyte-like cells (THP-1 Lucia ISG, Invivogen) were maintained in RPMI 1640(Thermo Fisher cat ...
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bioRxiv - Microbiology 2022Quote: A549-hACE2 lung carcinoma cells expressing the human ACE2 protein (Invivogen) were maintained in DMEM with 4.5 g/L glucose and 2 mM L-glutamine (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Immunology 2023Quote: ... IgG3 concentrations were measured by an IgG3 Human ELISA Kit (Invivogen).
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bioRxiv - Immunology 2023Quote: Human monocytes were stimulated with 10ug/mL TL8 (Invivogen, tlrl-tl8506), 10ug/mL ssPolyU(Invivogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Microbiology 2020Quote: ... filtered through sterile miracloth and pipetted on a double selective PDA plate containing 0.1 M Tris pH 8 supplemented with 100 μg/ml hygromycin (Duchefa) and 100 μg/ml zeocin (InvivoGen). Double drug resistant colonies were selected after three days and monospored on a new plate supplemented with both drugs ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by 2,5 μM BV6 (Selleckchem) pre-treatment (SK-N-AS cells only) and treatment with 10 μg/ml poly(I:C) (Invivogen). Human TNF-α (600 lU/ml ...
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bioRxiv - Immunology 2021Quote: ... Flow through sera was then applied to a gravity flow column containing 1 mL Peptide M-sepharose resin (InvivoGen) to purify IgA ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were selected with 2.5 µg/ml (PC3) and 5 µg/m (DU145) of blasticidin (InvivoGen ant-bl-1) for 7-9 days ...
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bioRxiv - Biochemistry 2019Quote: The vector containing human tlr2 gene (pUNO-hTLR2) was obtained from InvivoGen for gene manipulation ...
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bioRxiv - Microbiology 2021Quote: ... Human cGAS (hcGAS) and murine cGAS (mcGAS) plasmids were purchased from Invivogen. Flag-cGAS and V5-cGAS were amplified by PCR and were cloned into pcDNA3.2-DEST plasmids ...
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bioRxiv - Microbiology 2021Quote: ... generated from the human lung A549 cell line were purchased from InvivoGen SAS (Toulouse Cedex 4 ...
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bioRxiv - Immunology 2019Quote: ... RAW 264.7 macrophages stably expressing GFP-FL and hnRNP M-FL were transfected for 48 hours and then selected through addition of puromycin (Invivogen).
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bioRxiv - Immunology 2020Quote: ... 300µl of plasma was diluted with PBS heat-inactivated (56°C for 1 hr) and incubated with peptide M/Agarose (Invivogen) or Protein G/Agarose (GE lifeSciences ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2020Quote: Human embryonic kidney TLR5 knock-down cells (HEK-Blue-KD-TLR5 cells, Invivogen) were used for the in vitro experiments In these HEK-BLUE cells ...
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bioRxiv - Microbiology 2022Quote: Human embryonic kidney (HEK) 293 reporter cells for TLR2 and TLR4 (Invivogen, France) were exposed to the eluted dust samples to study their potential to induce NFkB responses ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with a human-mouse hybrid aCD20 (InvivoGen hcd20-mab10, 5 ng/ml), added to wells at 40,000 cells per well ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Sorted cells were kept for 2 days in E14 standard medium supplemented by 100 μg/m primocin (InvivoGen, ant-pm-1) and 10uM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... X293T (Human Embryonic Kidney cell line) and HEK-Blue IFN α/β cells (InvivoGen) were cultured in Dulbecco’s modified eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2023Quote: ... A549 lung carcinoma cells expressing human ACE2 (M08-0801, ©InvivoGen, San Diego, USA) were cultivated in DMEM (Gibco® ...
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bioRxiv - Microbiology 2021Quote: ... The 279bp human AldA enhancer was PCR amplified from the pDRIVE5-GFP-10 plasmid (Invivogen). The HCMV enhancer was PCR amplified from the pDrive5 GFP-1 Promoter Test (Invivogen) ...
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bioRxiv - Immunology 2021Quote: ... IgA+ cells were detected by addition of goat anti-human IgA F(ab’)2 (InVivoGen) followed by anti-goat IgG AlexaFluor 594 (Jackson Immunoresearch ...
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Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1bioRxiv - Biochemistry 2023Quote: ... Plasmids were transformed into SMD1163 by electroporation and grown on YPDS (1% yeast extract, 2% peptone, 2% dextrose, 1 M sorbitol) zeocin (InvivoGen, ant-zn-1p) plates ...
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec) and 5 μg/ml L-18-MDP (InvivoGen) + 10 ng/ml human interferon gamma ...
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bioRxiv - Immunology 2021Quote: ... WIL2-S cells were opsonized with dilutions of anti-human CD20 – murine IgG2a (hcd20-mab10, InvivoGen) for 30 min followed by incubation with the diluted secondary antibody for 30 minutes and washed 3 times with HBSS ...
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bioRxiv - Immunology 2020Quote: ... Treatment of live leptospires was performed with 100 μg/mL of the human cathelicidin LL37 (Invivogen) in PBS for 2 hours.
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bioRxiv - Cancer Biology 2022Quote: ... We selected infected human cell lines with 1 mg ml-1 blasticidin (InvivoGen, #ant-bl-1) for 14 days or with 1 mg ml-1 puromycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... human γδT cells were pre-treated with p-JNK inhibitor (SP600125: 20uM, Invivogen, San Diego, CA) for 30min followed by IL-12/18/21 stimulation for 4 hours(43) ...
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bioRxiv - Genetics 2020Quote: The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were transfected with mscv2.2 vectors containing human or murine NLRC4 were using lipofectamine 2000 (Invivogen). Briefly ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Genomics 2019Quote: ... supplemented with 5% human platelet lysate (Cook Regentec, #G34936) and 50 µg/mL Primocin (InvivoGen, #ant-pm-1). hASCs were cultured in the same media composition until 70-80% confluency and detached using TrypLE Select (Life Technology ...
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bioRxiv - Cell Biology 2022Quote: A549 lung carcinoma cells or A549 cells expressing human ACE2 or ACE2 and TMPRSS2 were obtained from Invivogen (a549 ...
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bioRxiv - Immunology 2020Quote: ... and 100 µl CpG (Murine CpG, ODN 1826, Human CpG ODN 2006 for pig and rabbit, InvivoGen, USA) were both added at 1mg/ml in WFI ...
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bioRxiv - Immunology 2019Quote: Detection of bioactive human IFN-I was performed using reporter cell line HEK-Blue IFN-α/β (InvivoGen) as previously described12 ...
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bioRxiv - Immunology 2021Quote: Human cell line THP-1 monocytes – ASC speck reporter cells were purchased from Invivogen (#thp-ascgfp, Invivogen, France) and called THP-1-ASC-GFP ...
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bioRxiv - Immunology 2023Quote: ... A549 with TMPRSS2 and human ACE2 overexpression cell line that was used in immunostaining was purchased from InvivoGen company (Cat.#a549-hace2tpsa ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Neuroscience 2022Quote: ... HEK-Blue™-hTLR2 cell line (Co-transfected with human TLR2 and SEAP reported gene) was purchased from InvivoGen. These cells were grown in DMEM media supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and MHC-I blocking antibody (W6/32; 50 ug/ml) in RPMI supplemented with human serum and primocin (Invivogen). Cells were harvested and stained with anti-CD3 FITC antibody (UCHT1 ...