Labshake search
Citations for Invivogen :
51 - 100 of 100 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Class C CpG oligonucleotide ODN-2395 (Invivogen). They were dissolved in 50:50 DMSO:PBS and applied topically at 100µg per dose ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... and c-di-GMP (100 µM; InvivoGen). To determine whether STING homologs can bind to CDNs without non-specific electrostatic interactions ...
-
bioRxiv - Immunology 2022Quote: ... Mice were infected intranasally with 3×104 PFU of x31-PmpG-1 virus and boosted intranasally with 1μg PmpG-1303-311 peptide + 2μg CpG (ODN 1826, Invivogen) on days 14 and 28 (75) ...
-
bioRxiv - Bioengineering 2024Quote: The ability of 1V209-modified peptides to activate TLR7 was assessed using HEK-Blue mTLR7 reporter cells (InvivoGen) using the standard protocol provided by the supplier ...
-
bioRxiv - Biochemistry 2019Quote: ... and c-di-AMP were purchased from Invivogen. The following monoclonal antibodies for western blotting were purchased from Cell Signaling Technologies ...
-
bioRxiv - Immunology 2021Quote: ... Transfection of DNA and Poly(I-C) (InvivoGen) was performed with Lipofectamine 3000 (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... c-di-AMP (7.5 µg per dose) (Invivogen), or c-di-AMP (7.5 µg ...
-
bioRxiv - Immunology 2022Quote: ... 2×104 DCs were co-cultured for 3 days with 5×104 FACS sorted naïve OT-II CD4+ T cells in the presence of 1 µg/ml OVA peptide (OVA323-339, Invivogen) and 0.25 ng/ml TGFβ (recombinant mouse TGF-b1 ...
-
bioRxiv - Immunology 2022Quote: Mice were injected subcutaneously with a total volume of 200ul containing 100ug TRP2180-188 peptide and 50ug poly(I:C) (InvivoGen) formulated in in PBS ...
-
bioRxiv - Neuroscience 2019Quote: For active immunization 59 mice were immunized subcutaneously into the flanks with 200 µg MOG35-55 peptide (Anawa) emulsified in complete Freund’s adjuvant (CFA; InvivoGen) and 200 ng pertussis toxin (List Biological Laboratories Inc. ...
-
bioRxiv - Immunology 2020Quote: ... and 10mM HEPES (UCSF Media Core Facility) in the presence of OVA257-264 peptide (0.1nM) (Invivogen, San Diego, California) and IL-2 (100U/ml ...
-
Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... total splenocytes isolated from OT-II mouse spleens were cultured with OVA 323-339 peptide (1 mg/ml, InvivoGen) and recombinant murine IL-2 (20 ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... Flow through sera was then applied to a gravity flow column containing 1 mL Peptide M-sepharose resin (InvivoGen) to purify IgA ...
-
bioRxiv - Neuroscience 2021Quote: ... were mixed well and heated to 50°C (or 65-70°C in the case of Invivogen high molecular weight poly I:C) in order to solubilize and melt dsRNA and then cooled to room-temperature to allow re-annealing before freezing in 1 ml aliquots and storing at −20 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
-
bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (10 μL each site) in both legs with 1 mg/mL OVA323–339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
-
bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
-
bioRxiv - Immunology 2021Quote: ... host mice were immunized subcutaneously in the hock area of both legs with 50 μL OVA323-339 peptides (1 mg/mL, InvivoGen) emulsified with complete Freund’s adjuvant (CFA ...
-
bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
-
bioRxiv - Immunology 2022Quote: ... 50,000 T cells were co-cultured with 10,000 sorted APCs in 96-well V-bottom plates (Cellstar) in the presence or absence of 20 μg/mL OVA323-339 peptide (Invivogen). To assess cytokine production ...
-
bioRxiv - Immunology 2023Quote: ... whole splenocytes from OT-1 mice were electroporated on day 3 after initial activation with 1 µg/mL OVA 257-264 peptide (InvivoGen), and cells were further expanded for 4 days with 200 U/mL rhIL-2 (NCI) ...
-
The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
-
bioRxiv - Immunology 2021Quote: ... 4 µg c-di-UMP (Invivogen, San Diego, Californien, USA) or mock-electroporated using a Gene Pulser Xcell Electroporation instrument (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2020Quote: ... poly dA: dT (dsDNA) and poly I: C (dsRNA) (Invivogen) were transfected into cells using PEI transfection reagent (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... 6-7 week-old BALB/c mice were ordered from Invivogen/Envigo and were allowed to acclimatize for 10 days prior to experimentation ...
-
bioRxiv - Pathology 2022Quote: ... A commercial standard of c-di-AMP (InvivoGen, Hong Kong, China) was used to determine the concentration by comparing the peak areas.
-
bioRxiv - Immunology 2021Quote: ... 2’-3’-cGAMP and c-di-UMP were purchased from Invivogen. EFV was purchased from Bristol-Myers Squibb.
-
bioRxiv - Immunology 2024Quote: ... The adjuvant C-Di-GMP was purchased from InvivoGen (Toulouse, France).
-
bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
-
bioRxiv - Immunology 2024Quote: ... mice were intradermally injected with 50 μg of neoantigen or control peptide and/or 100 μg high molecular weight VacciGrade polyinosinic-polycytidylic acid (poly(I:C) (Invivogen Cat. No. vac-pic)) ...
-
bioRxiv - Cancer Biology 2022Quote: ... iDCs were activated overnight with class C ODN 2395 (InvivoGen, San Diego, CA) and then cultured with or without indicated antigen peptides at 37 C for 30 min ...
-
bioRxiv - Immunology 2021Quote: ... 10µg OVA plus 0.5µg c-di-GMP (both from InvivoGen, San Diego, CA)) 3 times per week for 7 weeks ...
-
bioRxiv - Cell Biology 2021Quote: ... at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen) and 5 µg.mL-1 Phleomycin (InvivoGen).
-
bioRxiv - Pathology 2020Quote: ... a truncated HSP27 peptide consisting of the C-terminal half of the peptide, (100 µg, 75 µL) mixed with an aluminum hydroxide gel adjuvant (Alhydrogel® 2%, Al, 25 µL; Invivogen vac-alu-250, San Diego, CA). One week after the commencement of E2 and/or vaccinations ...
-
bioRxiv - Immunology 2023Quote: ... The Class C CpG ODN_2395 sequence (5’-TCGTCGTTTTCGGCGCGCGCCG-3’) was obtained from Invivogen (#tlrl-2395). We used the crystal structure of mouse TLR9 extracellular domain (TLR9 ECD ...
-
bioRxiv - Bioengineering 2020Quote: ... with agitation (200 rpm) at 37 °C containing the antibiotic zeocin (InvivoGen, San Diego, CA, USA) at a concentration of 20 μg/mL ...
-
bioRxiv - Immunology 2022Quote: ... 2′3′-RR c-di-AMP (2′3′-RR-S2 CDA) (Invivogen cat. no. tlrl-nacda2r), 2′3′- cyclic-di-GMP-AMP (2′3′-cGAMP ...
-
bioRxiv - Molecular Biology 2019Quote: ... Bacteria were shaken at 37°C for 40 min and plated onto Zeocin (25 µg/ml, InvivoGen) agar plates ...
-
bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
-
bioRxiv - Immunology 2021Quote: ... the cells were pre-incubated for 30 min at 37°C with 5 μg/mL of anti-TLR2 monoclonal antibody (clone T2.5, InvivoGen) or with an isotype control (mIgG1 ...
-
bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated at 37°C for 24 hours before supernatants were collected and mixed with QuantiBlue reagent (Invivogen). Colour changes ...
-
bioRxiv - Microbiology 2021Quote: ... 10 µg/ml Tri-Lys (negative control for NOD1/NULL1) or 10 µg/ml MDP-control (MDP-c; negative control for NOD2/NULL2) (InvivoGen) were used as treatment controls ...
-
bioRxiv - Microbiology 2022Quote: ... with 10 μg of A/Michigan/45/15 (N1) or B/Malaysia/2506/04 NA adjuvanted with 10 μg of poly(I⋅C) (Invivogen). Four weeks after the prime ...
-
bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
-
bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
-
bioRxiv - Immunology 2023Quote: ... we immunized two groups of BALB/c mice (n=10) with 5 µg protein antigens adjuvanted with 10 µg Monophosphoryl lipid A (MPLA, Invivogen) and 10 µg Quil-A (Invivogen ...
-
bioRxiv - Microbiology 2023Quote: ... Unlabeled standards (purity ≥95%; all from Sigma-Aldrich, except for XMP from Santa Cruz Biotechnology and c-di-GMP from InvivoGen) were diluted in the same solvent as samples for calibration curves.
-
bioRxiv - Cell Biology 2022Quote: ... Twenty mL of HEK-Blue™ supernatants were transferred to a 96-well plate containing 180 mL pre-warmed (37°C) QUANTI-Blue™ (the substrate for SEAP, InvivoGen, rep-qbs). Reactions were developed at 37°C for 50-90 min and measured at 630 nm using FLUOstar Omega Microplate Reader (BMG Lactech) ...
-
bioRxiv - Microbiology 2022Quote: ... extraction buffer supplemented with a final concentration of 0.01% formic acid and 25nM c-di-GMP-Fluorinated internal standard (InvivoGen CAT: 1334145-18-4). Pelleted cells were mechanically disturbed with the Qiagen TissueLyser LT at 50 oscillations/second for 2 minutes.