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Citations for Invivogen :
901 - 950 of 955 citations for MEK Kinase Kinase 1 MAP4K1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... with penicillin-streptomycin (100 units/mL) and activated using 3 µM CpG (ODN 7909) (tlrl-2006-1, Invivogen, San Diego, CA, USA). Cells were cultured in 200 µl medium for 1-8 days using round bottom 96-well plates ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
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bioRxiv - Immunology 2022Quote: ... stimuli were as follows: 1×108 pfu/well of purified phage preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). The TLR3 signalling inhibitor (EMD Millipore ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of CK2α recombinant DNA encoding residues 1 to 336 was subcloned into an in-house knob-and-hole pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the library were measured by mCherry flow cytometry and selected with 1 μg/ml puromycin (Invivogen, ant-pr). The selected cells were expended in culture for 13-14 doublings to obtain enough number of cells for xenograft experiments.
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bioRxiv - Immunology 2022Quote: A single dose consisted of 10 μg of peptide (peptide stock is 10 mg/mL resuspended in DMSO) was added to 25 μg cyclic-di-GMP (stock is 1 mg/mL resuspended in PBS) (Invivogen tlrl-nacdg) and PBS added to a final volume of 50 μL ...
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bioRxiv - Developmental Biology 2023Quote: ... Media was removed prior to the addition of heat-inactivated serum diluted 1:20 in HEK Blue Detection media (Invivogen, #hb-det3). Cells were incubated for 48 hours and then absorbance was measured at 630 nm with a plate reader (SpectraMax) ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Cancer Biology 2023Quote: ... The transfection mix was applied to the cells for six hours then removed and replaced with lentiviral collection media: DMEM + 10% FBS(HyClone) + 1.1% BSA + HEPES (10 mM) + sodium pyruvate (10 mM) + Primocin (Invivogen ant-pm-1). The lentivirus was collected in two batches at 48 and 72 hours and pooled together ...
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bioRxiv - Bioengineering 2023Quote: ... the mice were intratumorally injected with 40 μg of STINGΔTM protein mixed with 1 μg of 2’3’-cGAMP (InvivoGen, Cat # tlrl-nacga23-02) in 25 μL sterile PBS ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was transfected into Huh7 cells using TransIT LT-1 transfection reagent and after selection with puromycin dihydrochloride (Invivogen, CA, USA), a single colony was isolated using cloning cylinders (Merck ...
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bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
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bioRxiv - Biochemistry 2024Quote: ... CD8+ T cell depletion was done for one treatment group (targeted HC-HAdV5HVR7, encoding for li-OVA.1+2 + muIL2v) with 0.2 mg mCD8-mIgG2 (InvivoGen 10354-42-01) via intraperitoneal injection per mouse and application ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were then sorted for GFP-positive to enrich for CROP-seq-vector-positive cells and plated on matrigel-coated plates in mTesr1supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and Y-27632 (final concentration 5µM) ...
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bioRxiv - Molecular Biology 2021Quote: ... Parent Flp-In™ T-REx™-293 cells were additionally supplemented with 15 μg/ml blasticidin (InvivoGen, cat n°ant-bl-1) and 100 μg/ml zeocin for cultivation ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells were re-plated to a 75 cm2 flask in culture medium supplemented with 20 µg/mL blasticidin (InvivoGen, ant-bl-1) to begin the antibiotic selection.
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bioRxiv - Immunology 2020Quote: ... T cell independent type 1 (TI-I) stimulation was mimicked by incubation with CpG OND Type B or R848 (InvivoGen, San Diego, USA). TI type 2 (TI-II ...
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bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Developmental Biology 2022Quote: ... dTomato-positive cells were sorted on a BD FACS Aria III using BD FACSDiva 8.0.1 Software using a 100 µm nozzle and cultured on Martigel-coated plates in mTeSR Plus supplemented with 100 µg/ml Primocin (InvivoGen, ant-pm-1) and 25 µm Y-27632 ...
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bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Cancer Biology 2020Quote: Tumor tissue is collected in Basis medium (Advanced DMEM F12 Serum Free medium (Thermo, 12634010) containing 10mM Hepes (Thermo,15630080), 2mM GlutaMAX supplement (Thermo, 35050061) and 100 μg/ml Primocin (InVivoGen, ant-pm-1)) ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Neuroscience 2019Quote: ... 400 μg of conjugate fentanyl-TT hapten was mixed with 600 μg CpG ODN 2006 (Eurofins Genomics, Louisville, KY) and 1 mg Alhydrogel adjuvant 2% (InvivoGen, San Diego, CA) for 30 min and then refrigerated for 24h prior to IM administration at approximately 1.2 ml per monkey ...
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bioRxiv - Physiology 2021Quote: ... Duodenal or jejunal segments (∼6 cm) were isolated and placed into falcon tubes containing cold DMEM (high glucose, 4.5 g/l) containing Primocin (Invivogen, cat# ant-pm-1). Intestinal epithelial cells were dissociated using a Gentle Cell Dissociation Reagent (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the targeting vectors and incubated for 2 days before addition of selective medium containing 1.5 μg/ml puromycin (Invivogen, cat# ant-pr-1). After 5 days ...
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bioRxiv - Cancer Biology 2022Quote: Cells were plated on glass-teflon microscope slide (Tekdon, #518plain) and the next day treated with either DMSO or Phleomycin (InvivoGen # ant-ph-1) for 1 hr ...
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bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 hESC cells with high expression of sgRNAs (fused with mCherry) were enriched by G418 selection (1000 μg/ml, InvivoGen, ant-gn-1) for 11 days and flow cytometry sorting ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2023Quote: ... We used a variety of inhibitors including Torin 1 (inh-tor1) and Bafilomycin A1 (tlrl-baf1) both purchased from InvivoGen (San Diego, CA). INK128 (S2811) ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen, San Diego, CA) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... A2780 cells were transduced with pLVX-TetOne-MDM4-Puro and selected for with 1.5 μg/mL puromycin (InvivoGen, cat. no. ant-pr-1). Tight inducibility was confirmed through doxycycline (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 1 mg/kg of lipopolysaccharide (LPS endotoxin from E. coli 0111:B4 ultra-pure; InvivoGen, San Diego, CA) dissolved in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... T47D and MCF7 cells were transduced with the lentiviral particles and stably expressing cells were generated after selection using G418 (InvivoGen ant-gn-1) for two weeks ...
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bioRxiv - Developmental Biology 2024Quote: ... The chemical inhibitor or DMSO solution was replaced with fresh solution in the morning of 4 dpf and 5 dpf. Caspase-1 inhibitor - InvitroFit™ Ac-YVAD-cmk (Garcia-Calvo et al. 1998) (InvivoGen inh-yvad) was dissolved in DMSO and used at 20µM final working concentration ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Immunology 2021Quote: ... NLRP3-/- iBMDMs or NLRP3-/- iBMDMs reconstituted with mScarlet-tagged NLRP3 were seeded at 0.5×106 cells/well on a 12-well tissue culture plate and on the next day treated or not with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h or with 1 μg/ml LPS for 2.5 h and additionally with 20 μM MCC950 (Millipore Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 1% (v/v) nonessential amino acid (Multicell Wisent Inc., St-Bruno, QC, Canada) and 5μg/mL of Plasmocin® (Invivogen, San Diego, CA, USA), to prevent mycoplasma contamination ...
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bioRxiv - Plant Biology 2021Quote: ... Transgenic Col-0 (LUC) plants were selected on 1/2 MS medium supplemented with 50 μM hygromycin B (InvivoGen, Cat No. ant-hg-5). LUC activity was confirmed as described above ...
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated for 24 hours with 1×108 pfu/well of purified Pf4 phage or equivalent volume ΔPf4 preparation and 100 ng/ml LPS (Invivogen, Cat. No. tlrl-eblps). Cells were centrifuged at 300 x g for 5 minutes and supernatant was collected and used for cytokine profiling through Luminex (The Human Immune Monitoring Center ...
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bioRxiv - Developmental Biology 2024Quote: ... The EGFP-fused lineage barcode was confirmed to exist as a single copy in the genome and to be highly expressed after blasticidin selection (15 g/ml, InvivoGen, no. ant-bl-1) and flow cytometry sorting ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.