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Citations for Invivogen :
851 - 900 of 1010 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... or PAL-CRID3 (0.001-100 µM) for 30 min and then stimulated with 5 μg mL−1 nigericin (Invivogen) for 30 min.
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bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
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bioRxiv - Immunology 2021Quote: ... cells were pretreated for 1 hour with Chloroquine or CU-CPT9a or stimulated with TLR agonists (all from Invivogen).
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bioRxiv - Microbiology 2021Quote: ... Two days post-transfection the media was replaced with DMEM containing 0.5% fetal bovine serum and NOD1 or NOD2 agonist [1 μg/ml C12-iE-DAP (Invivogen)+ 10 ng/ml human interferon gamma (AbD serotec ...
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bioRxiv - Microbiology 2020Quote: ... DNA ratio of 3:1 according to manufacturer’s instructions or with Poly(I:C) (LMW)/LyoVec™ (tlrl-picwlv, InvivoGen). The treatment was administered as indicated ...
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bioRxiv - Cancer Biology 2021Quote: ... LPS-EK (1 μg/mL) and ODN2006 (10 μg/mL) were purchased from InvivoGen (tlrl-kit1hw and tlrl-pslta). Calf thymus DNA was purchased from Sigma (D1501) ...
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bioRxiv - Immunology 2022Quote: ... 2×104 DCs were co-cultured for 3 days with 5×104 FACS sorted naïve OT-II CD4+ T cells in the presence of 1 µg/ml OVA peptide (OVA323-339, Invivogen) and 0.25 ng/ml TGFβ (recombinant mouse TGF-b1 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa EGFP-CHIP and HEK EGFP-CHIP cells were supplemented with blasticidin (10 µg/ml) (ant-bl-1, Invivogen) and hygromycin B (50 µg/ml ...
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bioRxiv - Microbiology 2020Quote: ... IFN-β promoter activity was induced by transfecting 250 ng of poly I:C (average size 0.2-1 kb) (InvivoGen) during 16 h ...
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bioRxiv - Cell Biology 2021Quote: Endogenously tagged cell lines were maintained in HMI-9 supplemented with 20 % (v/v) FCS at 37 °C in 5% C02 and maintained in 10 µg.mL-1 Blasticidin (InvivoGen). Parasite density was monitored using a haemocytometer.
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bioRxiv - Immunology 2022Quote: ... d6-monocytes were stimulated with either 3pRNA as described above or 1 μg/ml P3CSK (InvivoGen, San Diego, USA) 5h prior to ATP challenge ...
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bioRxiv - Pathology 2019Quote: Spleen B cells were isolated and stimulated in vitro (1×106 cell/mL) with 1µg/mL of LPS (InVivoGen) for four days ...
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bioRxiv - Microbiology 2021Quote: ... Long-term passaging of landing pad cells utilized D10-dox with 20 μg/mL blasticidin (InvivoGen, ANT-BL-1) to remove cells with silenced landing pad loci.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The selection was started 48 hours after transfection using growth media supplemented with 500µg/ml Hygromycin Gold (ant-hg-1, Invivogen). Selection media was changed every 3-4 days until confluent colonies of stable transgenic cells had formed and cells were ready to be split.
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... total splenocytes isolated from OT-II mouse spleens were cultured with OVA 323-339 peptide (1 mg/ml, InvivoGen) and recombinant murine IL-2 (20 ng/ml ...
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bioRxiv - Immunology 2021Quote: Enriched human memory B cells were cultured at 0.2 x 106 cells per ml in complete RPMI supplemented with 1 mg/ml R848 (Invivogen), 1 mg/ml soluble CD40L (Fitzgerald) ...
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bioRxiv - Immunology 2021Quote: ... Flow through sera was then applied to a gravity flow column containing 1 mL Peptide M-sepharose resin (InvivoGen) to purify IgA ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2022Quote: ... Recipient mice were immunized intra-peritoneally (i.p.) with a 200 μl solution composed of 1 part of Alum (Invivogen, Alhydrogel® adjuvant 2% ...
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bioRxiv - Immunology 2022Quote: 2 × 106 pleura cells were plated and stimulated with 1 µg/mL poly(I:C) (Invivogen, San Diego, CA, USA) for 4 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... 1 µg purified S-EABR eVLPs were administered IM in the presence of 50% v/v AddaVaxTM adjuvant (Invivogen). Serum samples for ELISAs and neutralization assays were obtained on indicated days.
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bioRxiv - Microbiology 2023Quote: ... cells were placed under drug selection in 10% FBS/RPMI containing 1 µg/ml puromycin (InvivoGen, Cat# ant-pr) or 6 ng/ml blasticidin (InvivoGen ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-coated with laminin for 24 hr prior to treatment with MCC950 (1 μM, 2 hr, Invivogen, inh-mcc) followed by 15 min exposure to isoproterenol (100 nM) ...
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bioRxiv - Genomics 2022Quote: ... After 2 to 6 minutes of gentle manual shaking in ice cold PBS with 1% (v/v) primocin (InvivoGen), the intestinal pieces were inspected microscopically (x100 magnification ...
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bioRxiv - Developmental Biology 2024Quote: All cells were cultured in DMEM/F12 containing 10% FBS and Primocin (50 ng/ml) (InvivoGen, ant-pm-1). DPP4+ ...
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bioRxiv - Biochemistry 2023Quote: Jurkat-Lucia TCR-hPD-1 effector cells and Raji-APC-hPD-L1 antigen presenting cells were obtained from Invivogen as components of the PD-1/PD-L1 Bio-IC assay (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants from THP-1 Dual cells (20 µl) were mixed with QUANTI-Luc 4 Lucia/Gaussia reagent (Invivogen), and luminescence was measured by a luminometer ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were selected with 2.5 µg/ml (PC3) and 5 µg/m (DU145) of blasticidin (InvivoGen ant-bl-1) for 7-9 days ...
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bioRxiv - Immunology 2023Quote: ... a potent inhibitor of the noncanonical IkB kinases TANK-binding kinase-1 (TBK1) and IκB kinase-ε (IKKε) (Invivogen) were diluted in DMSO ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were verified as >95% pure by SDS-PAGE and contained <0.01 endotoxin units ml-1 by HEK-Blue TLR4 reporter assay (InvivoGen).
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bioRxiv - Cell Biology 2020Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Immunology 2021Quote: The transduced THP-1 cells were differentiated with 200 nM PMA for 24 h and treated with DOX (Wako) or nigericin (InvivoGen) at the indicated concentrations ...
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bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (10 μL each site) in both legs with 1 mg/mL OVA323–339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Immunology 2021Quote: ... For control purposes cells were stimulated with cytosolic 1 µg LPS using lipofectamine or nigericin (6.7 µM) (InvivoGen, tlrl-nig). 1% triton X100 was used as a lysis control in cell death assays (37) ...
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bioRxiv - Molecular Biology 2019Quote: The enhancer/promoter region of the chicken vitellogenin II gene spanning nucleotides −638 to −1 was cloned into a CpG-free firefly luciferase plasmid (pCpGL-basic, InvivoGen) using HindIII and NcoI ...
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bioRxiv - Microbiology 2021Quote: ... Vero and 293T GFP-split cells transduced cells with pQCXIP were cultured with 4ug/ml and 1 ug/ml of puromycin (InvivoGen), respectively ...
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bioRxiv - Microbiology 2020Quote: ... were subcutaneously immunised with ZIKV antigen formulated in aluminium hydroxide gel (1% ALUM, Brenntag) combined with 5 µg monophosphoryl lipid A (MPLA)(InvivoGen) or PBS containing the adjuvant ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Thermo Fischer Scientific) and 5 μg/ml blastidicin (BSD; InvivoGen). MEXi 293E cells (IBA Lifesciences ...
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bioRxiv - Immunology 2020Quote: Neutrophils isolated from 4 healthy volunteers were plated at 50,000 cells per well and stimulated in duplicate with the following ligands for 1 hour: lipotechoic acid (LTA) 100ng/mL (Invivogen), LPS (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Immunology 2021Quote: ... host mice were immunized subcutaneously in the hock area of both legs with 50 μL OVA323-339 peptides (1 mg/mL, InvivoGen) emulsified with complete Freund’s adjuvant (CFA ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1×106 T cells per well in RPMI medium supplemented with Primocin (100 µg/ml; Invivogen), 10 % charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2 and A549-S cell lines were selected using 10 μg/ml puromycin (InvivoGen, catalog no. ant-pr-1) 4 days post-transduction ...
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bioRxiv - Cell Biology 2019Quote: ... The U2OS USP42 KO cell line and its complemented cell lines stably expressing either GFP or GFP-USP42 (FL, ΔC or Δ946-1196) were cultured with identical media with 1 μg/ml puromycin (InvivoGen) or with 1 μg/ml puromycin and 500 μg/ml Geneticin ...
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bioRxiv - Immunology 2019Quote: ... 10% L929-conditioned media and 1% P/S supplemented with 100 ng/mL crude LPS (InVivogen, San Diego, CA, USA) and 20 ng/mL IFNγ (R&D Systems ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... for 72 h in the presence or absence of 1 µg/ml LPS-EB ultrapure (InvivoGen, San Diego, California, US). 2,4-Dinitrophenol (DNP ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Immunology 2021Quote: ... route in the deltoid muscle with a total volume of 1 ml of vaccine formulation containing 50 μg of RBD-trimer admixed with AddaVax adjuvant (InvivoGen) at weeks 0 ...