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Citations for Invivogen :
851 - 900 of 1004 citations for Flap endonuclease 1 FEN 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells that stably express human ACE2 (ACE2-293T) were cultivated in the presence of 2 μg/ml puromycin (Invivogen). Caco2 (human epithelial colorectal adenocarcinoma ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells stably expressing human TMPRSS2 were generated by lentiviral transduction followed by selection with blasticidin (40 μg/mL; Invivogen). SARS-CoV-2 strains hCoV-19/USA-WA1/2020 (NR-52281) ...
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bioRxiv - Molecular Biology 2023Quote: ... was fused to the Fc region (CH2 and CH3 domains) and hinge region of the human IgG1 heavy chain in the pFUSE-Fc1 vector (InvivoGen). Jag1ECD-Fc protein was expressed in Expi293F cells grown in Expi293F expression medium at 37°C in an 8% CO2 incubator with constant shaking ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2024Quote: Assessment of TLR4 and TLR5 activity was implemented using human embryonic kidney (HEK)-Blue-mTLR4 and HEK-Blue-mTLR5 cells respectively (Invivogen) using a protocol as previously described (Allen et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Immunology 2021Quote: The transduced THP-1 cells were differentiated with 200 nM PMA for 24 h and treated with DOX (Wako) or nigericin (InvivoGen) at the indicated concentrations ...
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bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (10 μL each site) in both legs with 1 mg/mL OVA323–339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Immunology 2021Quote: ... For control purposes cells were stimulated with cytosolic 1 µg LPS using lipofectamine or nigericin (6.7 µM) (InvivoGen, tlrl-nig). 1% triton X100 was used as a lysis control in cell death assays (37) ...
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bioRxiv - Molecular Biology 2019Quote: The enhancer/promoter region of the chicken vitellogenin II gene spanning nucleotides −638 to −1 was cloned into a CpG-free firefly luciferase plasmid (pCpGL-basic, InvivoGen) using HindIII and NcoI ...
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bioRxiv - Microbiology 2021Quote: ... Vero and 293T GFP-split cells transduced cells with pQCXIP were cultured with 4ug/ml and 1 ug/ml of puromycin (InvivoGen), respectively ...
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bioRxiv - Microbiology 2020Quote: ... were subcutaneously immunised with ZIKV antigen formulated in aluminium hydroxide gel (1% ALUM, Brenntag) combined with 5 µg monophosphoryl lipid A (MPLA)(InvivoGen) or PBS containing the adjuvant ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Thermo Fischer Scientific) and 5 μg/ml blastidicin (BSD; InvivoGen). MEXi 293E cells (IBA Lifesciences ...
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bioRxiv - Immunology 2020Quote: Neutrophils isolated from 4 healthy volunteers were plated at 50,000 cells per well and stimulated in duplicate with the following ligands for 1 hour: lipotechoic acid (LTA) 100ng/mL (Invivogen), LPS (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Host mice were immunized subcutaneously in the hock area (50 μL each site) in both legs with 1 mg/mL OVA323-339 peptide (InvivoGen) emulsified with CFA (InvivoGen) ...
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Immunology 2021Quote: ... host mice were immunized subcutaneously in the hock area of both legs with 50 μL OVA323-339 peptides (1 mg/mL, InvivoGen) emulsified with complete Freund’s adjuvant (CFA ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1×106 T cells per well in RPMI medium supplemented with Primocin (100 µg/ml; Invivogen), 10 % charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2 and A549-S cell lines were selected using 10 μg/ml puromycin (InvivoGen, catalog no. ant-pr-1) 4 days post-transduction ...
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bioRxiv - Cell Biology 2019Quote: ... The U2OS USP42 KO cell line and its complemented cell lines stably expressing either GFP or GFP-USP42 (FL, ΔC or Δ946-1196) were cultured with identical media with 1 μg/ml puromycin (InvivoGen) or with 1 μg/ml puromycin and 500 μg/ml Geneticin ...
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bioRxiv - Immunology 2019Quote: ... 10% L929-conditioned media and 1% P/S supplemented with 100 ng/mL crude LPS (InVivogen, San Diego, CA, USA) and 20 ng/mL IFNγ (R&D Systems ...
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Loss of p32 triggers energy deficiency and impairs goblet cell differentiation in ulcerative colitisbioRxiv - Molecular Biology 2020Quote: ... for 72 h in the presence or absence of 1 µg/ml LPS-EB ultrapure (InvivoGen, San Diego, California, US). 2,4-Dinitrophenol (DNP ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Immunology 2021Quote: ... route in the deltoid muscle with a total volume of 1 ml of vaccine formulation containing 50 μg of RBD-trimer admixed with AddaVax adjuvant (InvivoGen) at weeks 0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Treatment was initiated when tumors reached 5–6 mm in size and was given twice a week: 1 μg MPLA/tumor (#vac-mpls, InvivoGen) and indicated doses of mouse IFNγ (#485-MI/CF ...
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bioRxiv - Immunology 2019Quote: ... BMDMs were seeded at a density of 2 × 105 cells/well in 48-well plates and pre-stimulated with 500 ng ml-1 of LPS (tlrl-peklps; InvivoGen) for 3 h ...
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bioRxiv - Cell Biology 2019Quote: ... Stable transfectants were selected in medium containing 500 μg/ml hygromycin B (InvivoGen, cat. # ant-hg-1; San Diego, CA). Pools of resistant cell colonies were characterized for MT1-MMP expression by reverse transcription-PCR and Western blotting ...
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bioRxiv - Immunology 2020Quote: ... 300µl of plasma was diluted with PBS heat-inactivated (56°C for 1 hr) and incubated with peptide M/Agarose (Invivogen) or Protein G/Agarose (GE lifeSciences ...
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bioRxiv - Immunology 2021Quote: Primary human monocyte derived macrophages (hMDMs) or murine bone marrow-derived macrophages (BMDMs) were primed with 1 μg/mL or 400 ng/mL Pam3CSK4 (InvivoGen), respectively for 4 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and the protein was first adsorbed on 200 μg of aluminum hydroxide (alum; containing 100 μg of aluminum) before 20 μg of CpG1826 (vac-1826-1, Invivogen) were added at the point of injection ...
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bioRxiv - Immunology 2021Quote: 1×106 thawed PBMCs were incubated at 37 ⁰C and 5% CO2 during 18 hours in RPMI with 10% FBS without any stimuli or with 1 µM CpG-A (ODN 2216; InvivoGen). After incubation ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were seeded at 1 × 105 cells/well in 96-well U-bottom plates in media containing 500 ng/mL Pam3CSK4 (Invivogen) for 4-6 hours then treated with either Val-boroPro (10 μM ...
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bioRxiv - Immunology 2022Quote: ... immunization with 50 µg TNP-KLH prepared with 1/3 volume of either alum or aluminum hydroxide gel (alhydrogel, Invivogen); 20 µg recombinantly produced trimer-stabilized HA (see below ...
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bioRxiv - Cancer Biology 2022Quote: Mouse colonic organoids were maintained in maintained in advanced DMEM/F-12 supplemented with 1X Primocin (Invivogen #ant-pm-1), 10mM HEPES ...
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bioRxiv - Microbiology 2022Quote: VeroE6/TMPRSS2 (JCRB 1819) cells (Matsuyama et al., 2020) were propagated in the presence of 1 mg/ml geneticin (G418; Invivogen) and 5 μg/ml plasmocin prophylactic (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... and pKANA2CENPB followed by selection on hygromycin B (1 mg/mL, #089-06151, Wako) and blasticidin S (5 µg/mL, #ant-bl-05, InvivoGen).
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bioRxiv - Microbiology 2024Quote: ... A positive control for TNF-α or IL-1β release was also prepared by challenging cells with 1 μg/ml lipopolysaccharide (Escherichia coli 0111:B4; InvivoGen) for an equivalent time ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
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bioRxiv - Genomics 2023Quote: ... Cells were then imaged in the imaging buffer (0.5 μg/ml DAPI, 10 μg/ml Fungin (InvivoGen ant-fn-1) in PBS ...
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bioRxiv - Genetics 2024Quote: ... the pooled mixture of cells were expanded and subsequently maintained in D10-dox containing 1 μg/mL puromycin (InvivoGen, ANTPR).
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs at day 60 of differentiation were treated with 1-2 µM pevonedistat (Hycultec) or 750 ng MG-132 (InvivoGen) for three days ...
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bioRxiv - Immunology 2023Quote: ... Cat# tlrl-hprna), LyoVec (Cat# lyec-1) and dispersible whole glucan particles (WGP, Cat# tlrl-wgp) were purchased from InvivoGen. Recombinant murine IFN-β1 (Cat# 581304 ...
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bioRxiv - Immunology 2023Quote: ... wild-type BALB/c female mice were sensitized intraperitoneally twice at 7-day intervals with either PBS (Group A) or 40 μg of OVA (Group B-F) together with 1 mg of aluminum hydroxide adjuvant (InvivoGen) on day 0 and day 7 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...