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Citations for Invivogen :
751 - 800 of 806 citations for 2 Amino 6 chloro 9 3 5 di O p toluoyl beta D 2 deoxyribofurnanosyl purine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The K562 cell line was cultured in the same media than NK cells supplemented with 5 µg/mL of Plasmocin (InvivoGen) and was routinely tested for mycoplasma infection with Venor GeM Classic detection kit (Minerva Biolabs).
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bioRxiv - Biochemistry 2022Quote: ... and 100 μg/ml streptomycin supplemented with 15 μg/ml Blasticidin and 100 μg/ml Hygromycin (Invivogen, #ant-hg-5) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... Mice were immunized by intranasal administration of the quadrivalent HA vaccine containing 150 ng of each HA with or without 5 μg of lipopolysaccharide (LPS; InvivoGen), 5 μg of poly(I:C ...
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bioRxiv - Immunology 2020Quote: Total spleen cells were cultured at a density of 1 × 106 cells/mL in RPMI 1640 supplemented with 10% fetal calf serum (FCS) with 5 ng/mL LPS (Invivogen). For 4-hydroxytamoxifen (OHTAM ...
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bioRxiv - Neuroscience 2023Quote: ... The culture media was also supplemented with 0.2% (1:500 dilution from a 5 μg/ml working concentration) Plasmocin™ Prophylactic (ant. mpp; InvivoGen). The day of plating from cryopreservation was allocated as day 0 and 50% media changes were carried out every 2-3 days ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then pelleted (150 RCF, 5 min) and SEAP enriched supernatant was then utilized for detection media (QUANTI-Blue, Invivogen) activation.
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bioRxiv - Molecular Biology 2022Quote: ... Cell lines used for all mturboID-MS experiments were generated in HEK293 Flp-InTM T-RExTM cells as previously described14 and pool of stable transfectants selected with 200 μg/mL hygromycin (Multicell) and 5 μg/mL blasticidin (Invivogen). Expression of bait proteins were induced with 1 μg/mL tetracycline for 24 h ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred in a 10-cm culture dish and cultured in complete DMEM medium supplemented with 5-20 µg/ml of puromycin (InvivoGen) and 100-500 µg/ml geneticine disulfate (G418 ...
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bioRxiv - Cell Biology 2022Quote: ... and pKANA2CENPB followed by selection on hygromycin B (1 mg/mL, #089-06151, Wako) and blasticidin S (5 µg/mL, #ant-bl-05, InvivoGen).
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... OT-1 T-cells (5 × 104 cells/well) were co-cultured with 0.5 ng/mL SIINFEKL peptide (InVivoGen, # vac-sin)-pulsed splenocytes (5 × 104 cells/well ...
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bioRxiv - Cancer Biology 2024Quote: ... 24-well or 96-well plates at a concentration of 5×105 cells/mL in RPMI 1640 supplemented with 20% fetal calf serum and 1X primocin (Invivogen).
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bioRxiv - Immunology 2023Quote: ... we immunized two groups of BALB/c mice (n=10) with 5 µg protein antigens adjuvanted with 10 µg Monophosphoryl lipid A (MPLA, Invivogen) and 10 µg Quil-A (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... Figure 1A) and were sensitized intraperitoneally twice at 5-day intervals with either PBS or 10 μg of Ovalbumin (OVA, InvivoGen) together with 1 mg of aluminum hydroxide adjuvant (InvivoGen ...
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bioRxiv - Microbiology 2024Quote: ... Cells transduced with lentiviruses encoding for the different gRNAs were passaged in selection medium containing 10 µg/ml blasticidin and 5 µg/ml puromycin (ant-pr-1, InvivoGen) to obtain polyclonal cell populations lacking either GSDMD or GSDME ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from A3-5 were cultured with 250 ng/mL Poly(I:C)-LMW/LyoVec™ (PolyI:C; Invivogen, San Diego, USA) for 48 h at 37°C and 5% CO2 ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
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bioRxiv - Genomics 2024Quote: ... Hygromycin resistant colonies were selected on 1x Vogels+FGS media with 100 µg/mL hygromycin (cat# ant-hg-5; Invivogen). Genomic DNA was isolated from resultant colonies ...
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bioRxiv - Immunology 2024Quote: ... PlatinumE cells were selected for 5 days using 1 µg/ml puromycin and 10 µg/ml blasticidin S (both Invivogen) and subsequently cultured ...
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bioRxiv - Immunology 2024Quote: ... THP-1 cells and MDMs were seeded at 5 x 105 cells/well and primed with 500ng/mL Pam3CSK4 (Invivogen) for 16-24 hours before coculture ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 days after transfection the cells were transferred to 10 cm dish and 4-5 days after transfection cells were selected with 100 μg/ml Hygromycin (InvivoGen). All CRIS-PITCh plasmids are summarized in Table S1.
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bioRxiv - Biochemistry 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in a humidified atmosphere and regularly tested for Mycoplasma contamination using a commercially available detection kit (InvivoGen). After thawing ...
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bioRxiv - Immunology 2024Quote: ... all cells were centrifuged for 5 minutes at 300 x g and the medium was replaced with myeloid medium containing 7 µg/mL puromycin (Invivogen) to select cells transduced for 72 hours.
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bioRxiv - Molecular Biology 2024Quote: ... Stably transfected 293 Flp-In T-REx derivative cell lines were cultured in a medium supplemented with 5 mg/mL Blasticidin (Invivogen) and 100 mg/mL Hygromycin B (Invivogen).
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells stably expressing GFP were cultured in RPMI 1640 containing 10% (v/v) FCS and 5 μg/ml blasticidin (InvivoGen). Mouse thymocytes were cultured in RPMI1640 medium containing 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... of the resulting vector into MDAMB231 or BY549 cells and further selection on 5 µg/ml puromycin (InvivoGen, Toulouse, France), for 10–15 days ...
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bioRxiv - Immunology 2021Quote: ... Mice (8 mice per group) were intramuscularly injected twice at four-week intervals with each VLPs (HA content=3 µg) with AddaVax™ adjuvant (Invivogen, San Diego, CA, USA) (Figure 2) ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Omicron Variant (B.1.1.529/BA.1) pLV-SpikeV11) and Omicron Variants (BA.4/BA.5) pLV-SpikeV13) were purchased from InvivoGen (San Diego, CA). The human T-Cell lymphoma Jurkat (E6-1 ...
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) Primocin™ (InvivoGen, San Diego, CA; ant-pm-1) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Genomics 2020Quote: ... The action of MAPK Kinases (MEK1 and MEK2) was blocked by the use of a selective inhibitor UO126 at 5 µM (Invivogen, CA, USA). The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h the medium was replaced with a selection medium supplemented with 500 μg/ml hygromycin (Invivogen. Cat# ant-hg-5). The cells that survived and proliferated in 500 μg/ml hygromycin were selected and were permanently cultured in a medium containing 500 μg/ml hygromycin ...
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bioRxiv - Immunology 2023Quote: ... 1×106 UCBMC were stimulated for 6 hours at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 0.5 μg/ml PMA and 5 μg/ml ionomycin (InvivoGen, San Diego, California). CD107a antibodies were added at the beginning of stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Immunology 2024Quote: ... mice were vaccinated with either prefusion-stabilized protein subunit vaccine based on the Wuhan-1 SARS-CoV-2 isolate (S-2P) through subcutaneous injection of either 0.5 µg or 5 µg of protein formulated with AddaVax (Invivogen, #vac-adx-10) or mRNA-1273 (WA-1 matched spike ...
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bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were infected with virus in the presence of 5 µM polybrene for two days and subsequently selected either through antibiotic selection with 1 µg/ml puromycin (InvivoGen, ant-pr), 10 µg/ml blasticidin (InvivoGen ...
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bioRxiv - Pathology 2020Quote: ... All constructs generated were stably transduced into previously CLCN5 silenced cells using lentiviral particles produced in HEK-293 cells and were subsequently selected with 400 μg/ml hygromycin (Invivogen, #ant-hg-5). Site-directed mutagenesis was performed with the QuikChange® II XL Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna).
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bioRxiv - Immunology 2023Quote: Five zebrafish housed in 400-mL beakers containing 200mL sterile tank water were immersed in high molecular weight polyinosine-polycytidylic acid (HMW poly I:C, InvivoGen Cat.# tlrl-pic-5) (50µg/mL final concentration in 200mL tank water ...
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bioRxiv - Cancer Biology 2022Quote: ... The final product was verified with Sanger sequencing from both directions and incorporated into cells using PEI transfection and selection with G418 (InvivoGen, ant-gn-5).
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bioRxiv - Developmental Biology 2024Quote: ... The chemical inhibitor or DMSO solution was replaced with fresh solution in the morning of 4 dpf and 5 dpf. Caspase-1 inhibitor - InvitroFit™ Ac-YVAD-cmk (Garcia-Calvo et al. 1998) (InvivoGen inh-yvad) was dissolved in DMSO and used at 20µM final working concentration ...
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bioRxiv - Cell Biology 2024Quote: ... Isogenic cells stably expressing LEXY from a doxycycline-sensitive promoter were selected by treatment with 200 µg/ml hygromycin B (InvivoGen, ant-hg-5).
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bioRxiv - Molecular Biology 2024Quote: ... culture of AP2-P-3HA-glmS was transfected at ring stage with 50 μg of pSLI plasmid (containing a blasticidin selection marker) and selected first with 5 μg/mL blasticidin (Invivogen ant-bl-05). Parasites reappeared approximately five weeks after transfection and positive selection for integration was performed via the addition of 400µg/mL G418 (Sigma G8168).
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bioRxiv - Cell Biology 2024Quote: ... Stable cell lines generated in this background were cultured with 5 µg/ml of blasticidin and 200 µg/ml of hygromycin B (Invivogen #ant-hg-1). To minimise background expression of the transgene ...