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Citations for Invivogen :
651 - 700 of 754 citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... we immunized three groups of C57BL/6 mice (n=9) with 5 µg protein antigens adjuvanted with 500 µg alum (Alhydrogel®, Invivogen) and 20 µg CpG (ODN 1826 ...
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bioRxiv - Biochemistry 2020Quote: ... parasites were cultured with anhydrotetracycline medium at 250 nM (aTc+, MilliporeSigma) for 2 days before blasticidin /aTc+ medium was used (blasticidin at 2.5 µg/mL, InvivoGen).
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Immunology 2021Quote: ... in the left and right mid-thighs with 50 µg MD39 and 500 µg alum (Alhydrogel adjuvant 2%; InvivoGen) per side ...
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bioRxiv - Neuroscience 2021Quote: ... endotoxin levels in exosomes and iPS-Mg medium were measured using the HEK Blue LPS detection kit 2 (InvivoGen) following manufacturers’ instructions ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... experiments were grown in Dulbecco’s modified Eagle’s medium DMEM supplemented with 10% fetal bovine serum and 50ug/ml normocin (ant-nr-2, Invivogen). HEK293 cells and other cell lines used were grown at 37°C and 5% CO2.
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bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-coated with laminin for 24 hr prior to treatment with MCC950 (1 μM, 2 hr, Invivogen, inh-mcc) followed by 15 min exposure to isoproterenol (100 nM) ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured for 2 weeks in the presence of 1 µg/mL puromycin (ant-pr-1, InvivoGen). The cells were single-cell-cloned using a limiting dilution method ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2022Quote: 2 × 106 pleura cells were plated and stimulated with 1 µg/mL poly(I:C) (Invivogen, San Diego, CA, USA) for 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... of filtered PAO1 supernatant or TSB and KGMTM-2 containing purified flagellin at 1μg/ml or vehicle (tlrl-pafla; from P. aeruginosa; InvivoGen) were used for treating hTCEpi cells for various durations as indicated in the figure legends.
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bioRxiv - Immunology 2022Quote: ... CASP8-/- × MLKL-/- and subsequently derived gene-deficient BLaER1 macrophages were stimulated with 2 ng/mL LPS from E.coli (Invivogen) for 18 h ...
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bioRxiv - Genomics 2022Quote: ... After 2 to 6 minutes of gentle manual shaking in ice cold PBS with 1% (v/v) primocin (InvivoGen), the intestinal pieces were inspected microscopically (x100 magnification ...
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bioRxiv - Molecular Biology 2024Quote: ... and stable donor cell lines expressing MCP-CD63 were cultured in the presence of 2 μg/ml puromycin (Invivogen).
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bioRxiv - Cancer Biology 2023Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) Primocin™ (InvivoGen, San Diego, CA; ant-pm-1) for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Human monocytes THP–1 cells were seeded at a density of 3×105 cells/ml and treated with 100 ng/ml phorbol myristate acetate (PMA, InvivoGen, San Diego, CA, Cat. No. tlrl-pma) for 24 hours for their differentiation to nascent (M0 ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Genomics 2020Quote: ... The action of MAPK Kinases (MEK1 and MEK2) was blocked by the use of a selective inhibitor UO126 at 5 µM (Invivogen, CA, USA). The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... hDaxx or ATRX were maintained in HFF cell culture medium additionally supplemented with 5 μg/ml puromycin (InvivoGen, San Diego, CA, USA). HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h the medium was replaced with a selection medium supplemented with 500 μg/ml hygromycin (Invivogen. Cat# ant-hg-5). The cells that survived and proliferated in 500 μg/ml hygromycin were selected and were permanently cultured in a medium containing 500 μg/ml hygromycin ...
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bioRxiv - Immunology 2023Quote: ... 1×106 UCBMC were stimulated for 6 hours at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 0.5 μg/ml PMA and 5 μg/ml ionomycin (InvivoGen, San Diego, California). CD107a antibodies were added at the beginning of stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Selection and maintenance of cells that underwent recombination started 24 h later by including hygromycin (50 µg/ml, Invivogen ant-hg-5) in the culture media ...
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bioRxiv - Immunology 2024Quote: ... mice were vaccinated with either prefusion-stabilized protein subunit vaccine based on the Wuhan-1 SARS-CoV-2 isolate (S-2P) through subcutaneous injection of either 0.5 µg or 5 µg of protein formulated with AddaVax (Invivogen, #vac-adx-10) or mRNA-1273 (WA-1 matched spike ...
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bioRxiv - Cell Biology 2024Quote: Differentiated CD11c+ CD1c+ cells from iPSC cell lines and CB donors were FACS sorted (Table 5) and stimulated with a combination of 1 µg/ml LPS (InvivoGen, #tlrl-smlps), 5 µg/ml HMW poly(I:C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were infected with virus in the presence of 5 µM polybrene for two days and subsequently selected either through antibiotic selection with 1 µg/ml puromycin (InvivoGen, ant-pr), 10 µg/ml blasticidin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... A total of five adjuvants were tested for their efficacy within the vaccine formulations: Aluminum hydroxide gel (alum) (Alhydrogel adjuvant 2%, InvivoGen), Polyinosinic-polycytidylic acid (poly (I:C ...
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bioRxiv - Cancer Biology 2021Quote: We transduced cells with lentivirus carrying KRAB-dCas9 and sgRNA targeting candidate progrowth enhancers and performed puromycin (2 μg/ml) (InvivoGen) for three days postelectroporation to select against non-transduced cells ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... H1-Hela+CDHR3 SETD3 KO cells that stably expressed the protein of interest were selected by treatment with 2 μg ml−1 puromycin (InvivoGen) along with non transduced cells as negative controls.
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bioRxiv - Cell Biology 2022Quote: ... U2OS media was replaced with fresh media and 24 hours later U2OS infected cells were selected with 2 µg/ml of puromycin (InvivoGen) for 72 hours.
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bioRxiv - Neuroscience 2021Quote: ... were a kind gift of David Pla-Martin and Francesc Palau 21 and were grown in growth medium containing 2 μg/ml puromycin (InvivoGen).
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... MHCI-specific ovalbumin SIINFEKL/OVA257-264 or MHCII-specific ovalbumin peptide OVA323-339 were added at 2 μg/ml (Invivogen). Phorbol 12-myristate 13-acetate (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide plasmid and PB transposase were lipofectamin transfected into parental SunTag cell lines and selected by puromycin (2 µg/ml, Invivogen) treatment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected Cells were incubated for 72 hours before selection with media containing 2 ug/mL Puromycin (Invivogen ant-pr-1). Cells were selected for at least 3 passages before fluorescence activated cell sorting (FACS) ...
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bioRxiv - Cancer Biology 2023Quote: ... In some cases, cells were first pre-treated for 1 h with following inhibitors (2 µM H-151, 10 µM MCC950 (InvivoGen)) before addition of the treatments ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... RD-SCARB2-KO20 and RD-ΔEXT1+hSCARB254 cells were maintained in DMEM supplemented with 10 µg/ml puromycin (58-58-2, InvivoGen). All infected cells were maintained in media supplemented with 2.5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... iPSC-CMs at day 60 of differentiation were treated with 1-2 µM pevonedistat (Hycultec) or 750 ng MG-132 (InvivoGen) for three days ...
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bioRxiv - Microbiology 2023Quote: ... S10-3 WT cells were transduced with pTRIPZ-Rab5/7/11-GFP or EGFP-LAMP1 and selected in cDMEM supplemented with 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2023Quote: ... cells were transferred to a 10 cm dish the day after transfection and selection with Puromycin (2 μg/mL, InvivoGen) was started within 48 h of transfection ...