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Citations for Invivogen :
601 - 650 of 692 citations for SKF 83566 hydrobromide CAS 108179 91 5 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... During this incubation cells were resuspended in DMEM supplemented with 10% (vol/vol) FBS and 1x Normocin (InvivoGen, San Diego, CA, USA) and 6,000 cells were dispensed onto each well loaded with medium for a final siRNA concentration of 20 nM ...
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bioRxiv - Microbiology 2020Quote: ... whereas selection for penDE-CP_DsRed and PpcbC-ble-tCYC1 co-transformation was done using medium containing 50 μg/ml phleomycin (Invivogen, San Diego, CA). When the CRISPRa vector was transformed using PEG-mediated transformation ...
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bioRxiv - Physiology 2020Quote: ... HEK-Blue™ mNOD1 (#hkb-mnod1) cells and HEK-Blue™ mTLR4 (#hkb-mtlr4) cells were obtained from InvivoGen (San Diego, CA). Antibodies for phospho-PKB (#4058 ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc fusion proteins were generated by cloning the PSG1a cDNA (UniProt KB-P11464) and PSG9 cDNA (NM_002784) into the pFuse-IgG1e3-Fc1 vector (InvivoGen, San Diego, CA). The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen) ...
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bioRxiv - Immunology 2020Quote: ... with penicillin-streptomycin (100 units/mL) and activated using 3 µM CpG (ODN 7909) (tlrl-2006-1, Invivogen, San Diego, CA, USA). Cells were cultured in 200 µl medium for 1-8 days using round bottom 96-well plates ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
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bioRxiv - Microbiology 2023Quote: ... A549-Cas9 cells were transduced with a lentivirus containing hACE2 and subjected to Zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA) at 72 h after transduction ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fujifilm Wako Pure Chemical Corp., Osaka, Japan) supplemented with 10% fetal bovine serum (FBS; biowest, Nuaillé, France) and 400 µg/ml G418 (InvivoGen, CA, USA) as previously described (Zhou et al. ...
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bioRxiv - Bioengineering 2024Quote: All materials for in vivo use were tested for endotoxin using the HEK-Blue mTLR4 reporter cell line (InvivoGen, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Immunology 2024Quote: ... Individual 7.6X mock formulations of TT and DT antigens with aluminum-phosphate adjuvant (Adju-Phos®, AP, purchased from InvivoGen, CA, USA) were prepared aseptically by combining calculated amounts of stock solution of each antigen with a stock AP to a final concentration of 114 Lf/mL TT ...
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bioRxiv - Immunology 2024Quote: ... FMs were treated with NT or buprenorphine in the presence or absence of inhibitors to: TLR4 (LPS-RS, 10μg/ml) (Invivogen, San Diego, CA); TLR2 (TL2-C29 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Immunology 2023Quote: ... we immunized three groups of C57BL/6 mice (n=9) with 5 µg protein antigens adjuvanted with 500 µg alum (Alhydrogel®, Invivogen) and 20 µg CpG (ODN 1826 ...
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were selected by at least one-week treatment with 150 ug/ml G418 or 10 μg/mL blasticidin (InvivoGen, San Diego, CA). Bulk populations of selected cells were used in this study ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Microbiology 2021Quote: ... in a 1:1 ratio with 75 μg alum and 7.5 μg monophosphoryl Lipid A (Alum/MPLA group) (Invivogen, San Diego, CA, USA) or 1:1 ratio with AddaS03 (AddaS03 group ...
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bioRxiv - Immunology 2022Quote: ... immunization with S Ptn associated with the adjuvants Poly(I:C) HMW (VacciGradeTM, 10 mg, batch #VPIC-34-05, InvivoGen, San Diego, CA, USA) and Alum/Alhydrogel 2% (lot #0001657855 ...
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bioRxiv - Microbiology 2022Quote: ... About 6-8-week-old C57BL/6 mice were immunized with 20 µg (50 µl) purified antigen in PBS using Addavax (Invivogen, San Diego, CA) as the adjuvant ...
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bioRxiv - Genomics 2022Quote: ... 2 mL STC was added and the protoplasts were mixed with 20 mL liquid MMS containing 0.3 % agar and 200 μg hygromycin mL-1 (InvivoGen, San Diego, CA, USA). The mixture was poured on MMS containing 0.6 % agar and 200 μg hygromycin mL-1 ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Physiology 2020Quote: ... Muramyl dipeptide (MDP) (Cat# tlrl-mdp) and ultrapure LPS from Escherichia coli O111:B4 (Cat# tlrl-3pelps) were from InvivoGen (San Diego, CA). Blood glucose was repeatedly measured via tail vein sampling using Accu-Chek Aviva blood glucometer from Roche Diagnostics (Missisauga ...
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bioRxiv - Immunology 2020Quote: ... THP-1-XBlue cells were cultured in complete RPMI medium with the addition of zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA). Prior to stimulation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Immunology 2020Quote: The cell lines A549-Dual (adenocarcinoma human alveolar basal epithelial cells) and RAW-Lucia ISG (RAW-mouse macrophages) (InvivoGen, San Diego, CA, USA) were cultured in DMEM (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BALB/cJ mice were immunized with 20 μg of PyCSP[NXA] formulated with 20% v/v of Adjuplex (Invivogen, San Diego, CA, USA) as an adjuvant in PBS at 0 ...
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bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... and age were immunized subcutaneously with 30 μg of S2P proteins in PBS in a 1:1 (v/v) mix with AddaVax adjuvant (InvivoGen, San Diego, CA) at weeks 0 ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...
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bioRxiv - Microbiology 2024Quote: ... Stimulation of the IFNL triggered the JAK/STAT/ISGF3 pathway and induced SEAP production which was measured by a colorimetric assay at 650 nm using Quanti-BlueTM solution according to the manufacturer’s instructions (InvivoGen, San Diego, CA, USA).
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Microbiology 2023Quote: IgA1 heavy chains were generated by cloning the variable regions of selected antibodies into the expression vector pFUSEss-CHIg-hA1 (Cat# pfusess-hcha1, Invivogen, San Diego, CA). Variable regions of the antibody coding sequences were PCR amplified using the IgG1 heavy chain expression plasmid as a template and custom primers that appended an EcoRI site and an NheI site at the 5’ and 3’ ends respectively ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Cell Biology 2023Quote: ... We used a variety of inhibitors including Torin 1 (inh-tor1) and Bafilomycin A1 (tlrl-baf1) both purchased from InvivoGen (San Diego, CA). INK128 (S2811) ...
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bioRxiv - Genomics 2022Quote: ... the tissue was longitudinally cut and subjected to incubation in 3 mM EDTA in ice cold PBS with 1% (v/v) primocin (InvivoGen, San Diego, CA) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... NF-κB activation was determined after 20 h of incubation according to the manufacturer’s instructions by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen, San Diego, CA, USA). The plates were incubated for 2 hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 1 mg/kg of lipopolysaccharide (LPS endotoxin from E. coli 0111:B4 ultra-pure; InvivoGen, San Diego, CA) dissolved in saline ...
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bioRxiv - Immunology 2023Quote: ... were immunized intramuscularly with 1.5 μg purified nanoparticle immunogen in 100 μl (50 μl in each hind leg) of 50% (v/v) mixture of AddaVax adjuvant (Invivogen, San Diego, CA) (Figure 1 and S1) ...
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bioRxiv - Microbiology 2024Quote: ... Human adenocarcinoma alveolar basal epithelial A549 cells overexpressing ACE2 and TMPRSS2 (‘A549-ACE2-TMPRSS2’) (Cat#: a549-hace2tpsa) was purchased from Invivogen (San Diego, CA). Human airway epithelial Calu-3 (Cat# ...
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bioRxiv - Immunology 2024Quote: ... in a round-shaped bottom 96-well plate in the absence or presence of CpG A (3 µM; InvivoGen, San Diego, CA, USA) for 3 hours ...
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bioRxiv - Neuroscience 2024Quote: ... cells were exposed to various concentrations (0 to 10 µg/mL) of muramyl dipeptide (MDP, a bacterial cell wall component) (Cat. No. tlrl-lmdp, InvivoGen, San Diego, CA) for 24h ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μg mL-1 blasticidin S (no. 029-18701; Wako) or 100 µg mL-1 zeocin (no. 11006-33-0; InvivoGen, San Diego, CA) for the selection ...
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bioRxiv - Immunology 2022Quote: ... for 3 hr followed by (flagellin isolated from P. aeruginosa, 2 or 5 µg/mL) for 3 hr using FLA-PA Ultrapure (InvivoGen, tlrl-pafla) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 h the medium was replaced with a selection medium supplemented with 500 μg/ml hygromycin (Invivogen. Cat# ant-hg-5). The cells that survived and proliferated in 500 μg/ml hygromycin were selected and were permanently cultured in a medium containing 500 μg/ml hygromycin ...
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bioRxiv - Immunology 2023Quote: ... 1×106 UCBMC were stimulated for 6 hours at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 0.5 μg/ml PMA and 5 μg/ml ionomycin (InvivoGen, San Diego, California). CD107a antibodies were added at the beginning of stimulation ...