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Citations for Invivogen :
601 - 639 of 639 citations for 5 Benzyloxy pyrimidine 2 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... where n=2) were immunized with the indicated immunogen in 100 μL of 50% v/v of AddaVax™ adjuvant (Invivogen) and boosted with adjuvant on Day 14 ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Molecular Biology 2022Quote: ... Sorted cells were kept for 2 days in standard E14 medium supplemented by 100 μg/µl primorcin (InvivoGen, ant-pm-1) and 10 µM ROCK inhibitor (STEMCELL Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant IgA1 produced in ExpiCHO-S cells as described above was purified using protein M agarose beads (Cat# gel-pdm-2, InvivoGen US) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... treated with CM 1:1 diluted in fresh cell growth media or PC (positive control: 1 μg/ml TLR-2 ligand Pam3CSK4 and 100 nM TLR-9 ligand CpG ODN 1668, both InvivoGen, USA). For experiments with different glucose concentrations ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-ppp-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (Catalog Code, lyec-12, InvivoGen), following the manufacturer’ ss instructions ...
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bioRxiv - Bioengineering 2023Quote: ... or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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RORγt-dependent antigen-presenting cells direct regulatory T cell-mediated tolerance to food antigenbioRxiv - Immunology 2024Quote: ... with intraperitoneal injections of 100 μg of OVA mixed with 1 mg of Alum (1:1; Alhydrogel® adjuvant 2%; vac-alu-50; InvivoGen). Seven days after the final sensitization ...
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S HexaPro protein (1 µg for the first dose and 5 µg for the booster dose) was mixed with 100 µg of aluminium hydroxide (Invivogen, Cat#vac-alu-250). Serum was collected for analysis on study days 14 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 5 × 105 cells/mL in 200 μL of medium with 100 ng/mL LPS (InvivoGen, San Diego, CA, USA) and incubated at 37 °C for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were cultured at 37°C with 5% carbon dioxide and were confirmed to be negative for mycoplasma by testing with MycoStrip™ - Mycoplasma Detection Kit (InvivoGen; rep-mys-50) or Myco-Sniff Mycoplasma PCR Detection Kit (MP Biomedicals ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Pathology 2020Quote: ... FIV was diluted in PBS to 133μg/mL Coronavirus-specific protein and mixed 1:1 in 2% Alhydrogel (Invivogen vac-alu-250) to give a final concentration of 66.7μg/mL in 1% Alhydrogel ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Cell Biology 2023Quote: ... and if required relevant selection antibiotics (hygromycin 200 µg/ml ; InvivoGen, ant-hg-1 ; puromycin 2 µg/ml, InvivoGen, ant-pr-1). Im95m cells were routinely maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Guide-RNAs were stably transduced in SpCas9-expressing DC 2.4 cells using the LentiGuide-Neo vector and selected with 500 ug/mL of G418 (InvivoGen #108321-42-2). LentiGuide-Neo was a gift from Caroline Goujon (Addgene plasmid # 139449 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 6-well plate in 2 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr). Cells were trypsinized daily (typically 3 d ...
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bioRxiv - Immunology 2023Quote: ... Intraperitoneal sensitization: Mice were immunized intraperitoneally with 250 μg OVA and 250 μL Alhydrogel 2% (aluminum hydroxide [alum], InvivoGen, San Diego, CA), 40 μg CpG ...
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bioRxiv - Biochemistry 2024Quote: ... CD8+ T cell depletion was done for one treatment group (targeted HC-HAdV5HVR7, encoding for li-OVA.1+2 + muIL2v) with 0.2 mg mCD8-mIgG2 (InvivoGen 10354-42-01) via intraperitoneal injection per mouse and application ...
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bioRxiv - Neuroscience 2024Quote: ... which was composed of DMEM (Thermo, 41966) containing 10% Heat-inactivated FBS (HI-FBS, Cytiva, SH30071.03HI) and 2% primocin (Invivogen, ant-pm-1). The next day ...
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bioRxiv - Immunology 2021Quote: ... Cells reconstituted with pLenti CMVie-IRES-BlastR-based constructs were maintained with addition of 5 µg/ml and 1 µg/ml blasticidin (InvivoGen, Cat. no: ant-bl-05) for HEK293T and iBMDMs ...
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bioRxiv - Immunology 2024Quote: ... at 37°C under 5% CO2 and were routinely tested for mycoplasma contamination using MycoStrip™ detection kit (cat. no. rep-mys, InvivoGen, San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... immunogen suspensions were gently mixed 1:1 vol/vol with AddaVax adjuvant for immunizations 1 and 2 and O/W for immunization 3 (Invivogen, San Diego, CA) to reach a final concentration of 0.250 mg/mL antigen ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS cells were transfected with the targeting vectors and incubated for 2 days before addition of selective medium containing 1.5 μg/ml puromycin (Invivogen, cat# ant-pr-1). After 5 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen, San Diego, US) added at alternative passages ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Microbiology 2024Quote: ... low-passage vials of cells were thawed and incubated in Corning Falcon 75 cm2 vented culture flasks at a density of 2 x 106 / 15 mL of DMEM with 100 µg/mL Normocin (InvivoGen catalog # ant-nr) for approximately 72 hours ...
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bioRxiv - Biochemistry 2024Quote: Viral dsRNA-induced infection in cells was mimicked by treating the cells with 2 µg/mL of poly(I:C) LMW (InvivoGen, Cat. Code: tlrl-picw) dissolved in sterile PBS ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-PATC148 cells were obtained from MD Anderson Cancer Center and cultured in RPMI media supplemented with 2 uM glutamine and containing 100ug/mL primocin (Invivogen, Cat. no. ant-pm-1). Cell lines were routinely tested for mycoplasma and validated using STR profiling and mutational analysis.
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bioRxiv - Bioengineering 2024Quote: ... incubated overnight in R10 medium with 60 IU mL-1 IL-2 and 100 µg mL-1 Normocin™ antibiotic (Invivogen, CAT#ANT-NR-1). The next day ...
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bioRxiv - Bioengineering 2023Quote: ... 4 or 8 µg/ml) or ss-miRNA-21 (2, 4 or 8 µg/ml) were transfected into the cells using LyoVec™ (InvivoGen, Catalog Code. lyec-12, InvivoGen) following the manufacturer’ ss instructions ...
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bioRxiv - Pathology 2020Quote: ... a truncated HSP27 peptide consisting of the C-terminal half of the peptide, (100 µg, 75 µL) mixed with an aluminum hydroxide gel adjuvant (Alhydrogel® 2%, Al, 25 µL; Invivogen vac-alu-250, San Diego, CA). One week after the commencement of E2 and/or vaccinations ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...