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Citations for Invivogen :
551 - 600 of 686 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cells transduced with lentiviruses encoding for the different gRNAs were passaged in selection medium containing 10 µg/ml blasticidin and 5 µg/ml puromycin (ant-pr-1, InvivoGen) to obtain polyclonal cell populations lacking either GSDMD or GSDME ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from A3-5 were cultured with 250 ng/mL Poly(I:C)-LMW/LyoVec™ (PolyI:C; Invivogen, San Diego, USA) for 48 h at 37°C and 5% CO2 ...
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bioRxiv - Systems Biology 2023Quote: Hela-S3 cells were purchased from ATCC and cultured in DMEM supplemented with 10 % heat inactivated FBS without antibiotics and incubated at 37 °C and 5 % CO2 Cells were tested regularly for mycoplasma with the PlasmoTest kit (InvivoGen) and are mycoplasma negative ...
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bioRxiv - Genomics 2024Quote: ... Hygromycin resistant colonies were selected on 1x Vogels+FGS media with 100 µg/mL hygromycin (cat# ant-hg-5; Invivogen). Genomic DNA was isolated from resultant colonies ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... shControl and shLRBA HeLa cells were seeded at 0.3*106 cells in 6 well-plates followed by treatment for 16 h or 24 h with 5 μM of MG132 (InvivoGen) alone or in combination with 100 nM of Bafilomycin A1 for 3 h.
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bioRxiv - Immunology 2024Quote: ... PlatinumE cells were selected for 5 days using 1 µg/ml puromycin and 10 µg/ml blasticidin S (both Invivogen) and subsequently cultured ...
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bioRxiv - Immunology 2024Quote: ... THP-1 cells and MDMs were seeded at 5 x 105 cells/well and primed with 500ng/mL Pam3CSK4 (Invivogen) for 16-24 hours before coculture ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in a humidified atmosphere and regularly tested for Mycoplasma contamination using a commercially available detection kit (InvivoGen). After thawing ...
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bioRxiv - Immunology 2024Quote: ... all cells were centrifuged for 5 minutes at 300 x g and the medium was replaced with myeloid medium containing 7 µg/mL puromycin (Invivogen) to select cells transduced for 72 hours.
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bioRxiv - Immunology 2024Quote: Immune responses were induced in 6-12-week-old male and female mice by subcutaneous immunization in the right FP with 5 µg (for HA experiments) or 10 µg (for hapten-carrier experiments) supplemented with 1/3 volume alhydrogel adjuvant (Invivogen). In S1pr2-Tomato mice ...
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bioRxiv - Molecular Biology 2024Quote: ... Stably transfected 293 Flp-In T-REx derivative cell lines were cultured in a medium supplemented with 5 mg/mL Blasticidin (Invivogen) and 100 mg/mL Hygromycin B (Invivogen).
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells stably expressing GFP were cultured in RPMI 1640 containing 10% (v/v) FCS and 5 μg/ml blasticidin (InvivoGen). Mouse thymocytes were cultured in RPMI1640 medium containing 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... of the resulting vector into MDAMB231 or BY549 cells and further selection on 5 µg/ml puromycin (InvivoGen, Toulouse, France), for 10–15 days ...
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bioRxiv - Immunology 2022Quote: ... MDDCs were harvested and cultured over-night in media alone or in the presence of 5 μg/ml of a pool of 15-mer overlapping HIV-1 Gag peptides provided by the NIH AIDS Reagent Program (#11057) either individually or in combination with 1 μg/ml of 2’3’-c’diAM(PS)2 (Invivogen) STING agonist and 2.5 μg/ml of Poly I:C (SIGMA ...
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bioRxiv - Immunology 2021Quote: ... in a final volume of 100 µL X-VIVOTM-15 + 2% HS + 1 µg.mL-1 poly(I:C) (tlrl-picw, Invivogen). After 3 h ...
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bioRxiv - Immunology 2021Quote: ... Specific wells were pre-cultured for 2 hours with either 10mg/ml of TLR4 inhibitor (CLI-095, Invivogen) or 10mg/ml PI3 Kinase inhibitor (LY294002 ...
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bioRxiv - Microbiology 2020Quote: ... except that the protoplasts were transformed with 1μg of each split-marker cassette DNA and plated on medium containing 200 g.l−1 saccharose and 2 g.l−1 NaNO3 supplemented with 70 μg.ml−1 hygromycin (Invivogen, France) for single ΔBcflp1 or ΔBcflp2 mutants or 80 μg.ml−1 nourseothricin (Werner BioAgents ...
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bioRxiv - Bioengineering 2022Quote: ... 293-cov2-sdf) or 2) Delta/B.1.617.2 variant) (Catalogue code: 293-SARS2-S-V8-dfur) were purchased from Invivogen. The cells were grown in DMEM media containing 10% Fetal Bovine Serum ...
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bioRxiv - Immunology 2021Quote: ... injection of 10 mg/kg poly(I:C) for 6h followed by 2 mg/kg ultrapure LPS-B5 (InvivoGen) prepared from E ...
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bioRxiv - Genomics 2023Quote: ... cells were cultured in the same medium supplemented with 200 μg/ml HygromycinGold B (Invivogen ant-hg-2). To generate cells with constitutive BFP expression ...
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bioRxiv - Bioengineering 2023Quote: ... The transfected cells were expanded to T-75 flasks and cultured with 2 µg/mL of puromycin (Invivogen), 10 µg/mL of blasticidin (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stimulated by adding 40 ml of fresh medium containing 1µg/ml of 2’3’-cGAM(PS)2 (Invivogen) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... the cultures were incubated with or without a bacterial agonist cocktail (2ug/mL Pam3CSK4 (TLR1/2 agonist, InvivoGen), 1 ug/mL FSL-1 (TLR2/6 agonist ...
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bioRxiv - Microbiology 2023Quote: ... ACE2 and transmembrane serine protease 2 (TMPRSS2)-expressing A549 (ACE2-TMPRSS2-A549) cells were from Invivogen (#a549-hace2tpsa) and HEK293T were from ATCC (#CRL-11268) ...
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bioRxiv - Microbiology 2021Quote: ... extraction buffer supplemented with a final concentration of 0.01% formic acid and 25nM cyclic di-GMP-Fluorinated internal standard (InvivoGen CAT: 1334145-18-4). Pelleted cells were mechanically disturbed with the Qiagen TissueLyser LT at 50 oscillations/second for 2 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were routinely confirmed to be negative for mycoplasma infection every 3–4 months using PlasmoTest mycoplasma detection kit (InvivoGen, catalog # rep-pt1). No other authentication assay was performed.
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bioRxiv - Microbiology 2022Quote: ... extraction buffer supplemented with a final concentration of 0.01% formic acid and 25nM c-di-GMP-Fluorinated internal standard (InvivoGen CAT: 1334145-18-4). Pelleted cells were mechanically disturbed with the Qiagen TissueLyser LT at 50 oscillations/second for 2 minutes.
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Immunology 2023Quote: ... we immunized three groups of C57BL/6 mice (n=9) with 5 µg protein antigens adjuvanted with 500 µg alum (Alhydrogel®, Invivogen) and 20 µg CpG (ODN 1826 ...
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bioRxiv - Neuroscience 2021Quote: ... with half media changes every 2-3 days with Neurobasal (ThermoFisher #21103049 supplemented with Primocin (InvivoGen #ant-pm-1), B-27 (ThermoFisher #17504044 ...
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bioRxiv - Biochemistry 2020Quote: ... parasites were cultured with anhydrotetracycline medium at 250 nM (aTc+, MilliporeSigma) for 2 days before blasticidin /aTc+ medium was used (blasticidin at 2.5 µg/mL, InvivoGen).
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Immunology 2021Quote: ... in the left and right mid-thighs with 50 µg MD39 and 500 µg alum (Alhydrogel adjuvant 2%; InvivoGen) per side ...
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bioRxiv - Neuroscience 2021Quote: ... endotoxin levels in exosomes and iPS-Mg medium were measured using the HEK Blue LPS detection kit 2 (InvivoGen) following manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... were immunized with 3 ugs of purified RBD of SARS-CoV-2 mixed with 10 ugs of poly I:C (Invivogen) twice with 3 weeks interval (17 ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... experiments were grown in Dulbecco’s modified Eagle’s medium DMEM supplemented with 10% fetal bovine serum and 50ug/ml normocin (ant-nr-2, Invivogen). HEK293 cells and other cell lines used were grown at 37°C and 5% CO2.
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bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-coated with laminin for 24 hr prior to treatment with MCC950 (1 μM, 2 hr, Invivogen, inh-mcc) followed by 15 min exposure to isoproterenol (100 nM) ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: Cells were primed with 2 µg/mL (human primary Mo) or 10 µg/mL (BLaER1 Mo) of Pam3CSK4 (Invivogen) for 2 h before activation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured for 2 weeks in the presence of 1 µg/mL puromycin (ant-pr-1, InvivoGen). The cells were single-cell-cloned using a limiting dilution method ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Immunology 2022Quote: 2 × 106 pleura cells were plated and stimulated with 1 µg/mL poly(I:C) (Invivogen, San Diego, CA, USA) for 4 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... of filtered PAO1 supernatant or TSB and KGMTM-2 containing purified flagellin at 1μg/ml or vehicle (tlrl-pafla; from P. aeruginosa; InvivoGen) were used for treating hTCEpi cells for various durations as indicated in the figure legends.
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bioRxiv - Immunology 2022Quote: ... CASP8-/- × MLKL-/- and subsequently derived gene-deficient BLaER1 macrophages were stimulated with 2 ng/mL LPS from E.coli (Invivogen) for 18 h ...
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bioRxiv - Genomics 2022Quote: ... After 2 to 6 minutes of gentle manual shaking in ice cold PBS with 1% (v/v) primocin (InvivoGen), the intestinal pieces were inspected microscopically (x100 magnification ...
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bioRxiv - Molecular Biology 2024Quote: ... and stable donor cell lines expressing MCP-CD63 were cultured in the presence of 2 μg/ml puromycin (Invivogen).
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).