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Citations for Invivogen :
501 - 550 of 558 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... were vaccinated two times at 3 week intervals with 10 µg of recombinant protein or PBS control diluted at a 1:1 ratio with Addavax (InvivoGen) via the IP route ...
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bioRxiv - Immunology 2024Quote: ... PlatinumE cells were selected for 5 days using 1 µg/ml puromycin and 10 µg/ml blasticidin S (both Invivogen) and subsequently cultured ...
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bioRxiv - Immunology 2024Quote: Immune responses were induced in 6-12-week-old male and female mice by subcutaneous immunization in the right FP with 5 µg (for HA experiments) or 10 µg (for hapten-carrier experiments) supplemented with 1/3 volume alhydrogel adjuvant (Invivogen). In S1pr2-Tomato mice ...
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bioRxiv - Microbiology 2024Quote: ... after which alkaline phosphatase activity was measured by mixing 10 μl of the culture supernatant and 90 μl of Quanti-Blue (InvivoGen) and reading O.D ...
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bioRxiv - Genomics 2024Quote: ... were integrated into cells by Sleeping Beauty transposition then selected using 800 μg/mL G418 and 10 μg/mL blasticidin (InvivoGen). Monoclonal cell lines were isolated by sorting for cells constitutively expressing GFP and expressing BFP upon treatment with 1 μg/mL doxycycline (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Jurkat cells stably expressing GFP were cultured in RPMI 1640 containing 10% (v/v) FCS and 5 μg/ml blasticidin (InvivoGen). Mouse thymocytes were cultured in RPMI1640 medium containing 10% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK 293 T-REx FLAGXPO1 stable cell line maintained in the complete D-MEM culturing medium supplemented with 10 µg/ml blasticidin and 50 µg/ml hygromycin (InvivoGen). All transfections were carried out using either Lipofectamine2000 (plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... low-endotoxin immunogens were diluted in buffer and mixed with 1:1 v/v AddaVax adjuvant (InvivoGen vac-adx-10) to obtain a final dose of 1.5 μg of immunogen per animal ...
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bioRxiv - Immunology 2021Quote: ... K18-hACE2 mice of both sexes were primed and boosted at 3 weeks intervals with S (10 µg/dose) or RBD (20 µg/dose) emulsified with AddaS03™ (InvivoGen) via intramuscular route ...
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bioRxiv - Systems Biology 2020Quote: ... Fragments were rocked on ice with crypt isolation buffer (10 mM EDTA, Life Technologies, in PBS0 supplemented with 500X dilution of Primocin, Invivogen, 10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... 24 and a Blasticidin resistance gene used for the initial selection of infected cells (10 µg/ml Blasticidin, Invivogen, ant- bl). MEF cells were cultivated in DMEM (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Different innate immune stimuli were applied to cell medium at 10% culture volume as follows: High molecular weight (HMW) poly(I:C) (Invivogen #tlrl-pic) at a concentration of 1mg/mL for 2 h (final concentration in the well 100 ug/mL ...
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bioRxiv - Microbiology 2022Quote: ... Subcutaneous vaccination was performed by inoculation with 100 µL of vaccine solution containing 10 µg of ODN2006 (InvivoGen, San Diego, CA) or 1 mg of Imject Alum adjuvant (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... AddaVax (MF59-like adjuvant; squalene oil-in-water emulsion; Catalog # vac-adx-10) was purchased from Invivogen (San Diego, CA, USA). The SARS S protein vaccine ...
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bioRxiv - Immunology 2020Quote: ... 7-to 8-week old WT mice were injected intraperitoneally with 10 mg/kg body wight of high molecular weight poly I:C (InvivoGen, tlrl-pic). The mice were then administered intraperitoneally 200 μl of DPBS containing no antibody (n = 15) ...
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bioRxiv - Immunology 2020Quote: ... we added the Syk-selective tyrosine kinase inhibitor piceatannol (at 10 or 30 μM) (Invivogen, San Diego, CA, catalog # tlrl-pct) or the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% FCS and 5 µg/ml plasmocin prophylactic reagent (InvivoGen, Cat.: ant-mpp) at 37 °C/5% CO2 to induce better melanin production ...
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bioRxiv - Immunology 2022Quote: ... Freshly isolated PBMCs were stimulated with 10 μg/ml of whole glucan particles (WGP Dispersable or 1,3/1,6-β-glucan) (InvivoGen, tlr-wgp) which is a specific Dectin-1 agonist that lacks TLR stimulating activity--for 18 hours (for Monocyte experiments) ...
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bioRxiv - Molecular Biology 2024Quote: ... in the presence or absence (gB-C7 only) of CpG 1018 adjuvant (10 mg; Dynavax) and aluminum hydroxide (alum; Alhydrogel®; 50 mg; InvivoGen). Immunogens were prepared by first mixing gB protein with alum for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... All cell lines were negative for mycoplasma contamination as confirmed using a MycoStrip™ - Mycoplasma Detection Kit (InvivoGen rep-mys-10). FBS was heat inactivated prior to use at 56°C for 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were immediately transferred to LIT medium for 24 hours before adding 10 μg/mL puromycin (Invivogen, San Diego, California, USA) or 50 μg/mL blasticidin (Invivogen ...
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bioRxiv - Molecular Biology 2024Quote: ... consisting of RPMI 1640 with L-Glu media +10%FBS+1xAnti-Anti+10mM HEPES + 1µg/mL R848 (Cat# tlrl-r848, Invivogen, California, USA) + 50uM β-Mercaptoethanol at 37°C/5%CO2 ...
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bioRxiv - Microbiology 2024Quote: Cells were seeded at a density of 1.5×105 cells/well for 24 hours followed by transfection with 10 ug of poly(I:C) chemically labeled with rhodamine (Invivogen, #tlrl-picr) for 16 hours ...
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bioRxiv - Immunology 2024Quote: ... 2.5 μM, and 5 μM) or with the TLR8 inhibitor CU-CPT9a (2.5 μM, 5 μM, and 10 μM) (InvivoGen, #inh-cc9a) and incubated at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... burgdorferi at MOI 10 or mock-treated in the presence of c-di-AMP (1 µg/ml) (InvivoGen, catalog tlrl-nacda) or water for 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... and digested in 10 mL of digestion medium (DMEM/F12: Thermo Fisher Scientific 12634010, 1x Penicillin-Streptomycin-Neomycin: Sigma P4083, 1x Normocin: Invivogen ant-nr-2 ...
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bioRxiv - Immunology 2021Quote: 1×106 freshly thawed PBMC or 1×105 purified CD14+ monocytes were cultured in RPMI supplemented with 10% FBS with or without 100 ng/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) for 16 or 6 hours ...
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bioRxiv - Microbiology 2022Quote: ... 293-ACE2 cells were maintained in DMEM supplemented with 10% fetal bovine serum and 200 µg/mL G418 (Invivogen #ant-gn-2). HEK-Blue 293 hACE2-TMPRSS2 cells (Invivogen #hkb-hace2tpsa ...
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bioRxiv - Cell Biology 2020Quote: ... During this incubation cells were resuspended in DMEM supplemented with 10% (vol/vol) FBS and 1x Normocin (InvivoGen, San Diego, CA, USA) and 6,000 cells were dispensed onto each well loaded with medium for a final siRNA concentration of 20 nM ...
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bioRxiv - Cancer Biology 2020Quote: ... ≈80% confluent adherent cells were washed twice in 1× PBS and subsequently pulsed with puromycin-containing media (InvivoGen, 10 μg mL−1) for 10 min ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Microbiology 2021Quote: ... were performed with freshly adjuvanted (Sepivac SWE™ (Cat.No. 80748J, Batch no. 200915012131, SEPPIC SA, France) and/or (AddaVax™ (vac-adx-10, InvivoGen, USA)) ...
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bioRxiv - Immunology 2022Quote: ... 4.5 g/L Glucose, 1 mM Sodium Pyruvate, 10% FBS (Thermo Fischer, Massachusetts, US) and 100 μg/ml Normocin (Invivogen, California, US). Cells were grown in Nunc™ 75cm2 culture flasks at a maximum of 2×106 cells/ml with 100 μg/ml of selective antibiotic Zeocin (Invivogen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fujifilm Wako Pure Chemical Corp., Osaka, Japan) supplemented with 10% fetal bovine serum (FBS; biowest, Nuaillé, France) and 400 µg/ml G418 (InvivoGen, CA, USA) as previously described (Zhou et al. ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Immunology 2023Quote: ... 1×106 UCBMC were stimulated for 6 hours at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 0.5 μg/ml PMA and 5 μg/ml ionomycin (InvivoGen, San Diego, California). CD107a antibodies were added at the beginning of stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... were diluted to 20 µg/mL in 1x TBS (20mM Tris, 150mM NaCl, pH 8.0) and mixed with 1:1 vol/vol AddaVax (InvivoGen vac-adx-10) to reach a final dose of 1 µg of immunogen per injection ...
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bioRxiv - Cell Biology 2024Quote: Chemical compounds dissolved in sterile DMSO were used at the following concentration unless indicated otherwise: DMXAA STING agonist (Invivogen, 10 µg/mL), cGAS inhibitor (G150 ...
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bioRxiv - Microbiology 2024Quote: ... each mouse was intramuscularly injected with 50μL of a 2μg dose of recombinant protein diluted 1:1 with AddaVax (InvivoGen, Cat # vac-adx-10) in the hind leg ...
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bioRxiv - Neuroscience 2024Quote: ... which was composed of DMEM (Thermo, 41966) containing 10% Heat-inactivated FBS (HI-FBS, Cytiva, SH30071.03HI) and 2% primocin (Invivogen, ant-pm-1). The next day ...
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bioRxiv - Immunology 2024Quote: ... mice were vaccinated with either prefusion-stabilized protein subunit vaccine based on the Wuhan-1 SARS-CoV-2 isolate (S-2P) through subcutaneous injection of either 0.5 µg or 5 µg of protein formulated with AddaVax (Invivogen, #vac-adx-10) or mRNA-1273 (WA-1 matched spike ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were selected by at least one-week treatment with 150 ug/ml G418 or 10 μg/mL blasticidin (InvivoGen, San Diego, CA). Bulk populations of selected cells were used in this study ...
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bioRxiv - Immunology 2022Quote: ... immunization with S Ptn associated with the adjuvants Poly(I:C) HMW (VacciGradeTM, 10 mg, batch #VPIC-34-05, InvivoGen, San Diego, CA, USA) and Alum/Alhydrogel 2% (lot #0001657855 ...
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bioRxiv - Biophysics 2020Quote: ... and mixed with 50 μl of AddaVax™ adjuvant (vac-adx-10) (1:1 v/v Antigen: AddaVax™ ratio per animal/dose) (InvivoGen, USA). Immunizations were given by intramuscular injection on Day 0 (prime ...
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bioRxiv - Immunology 2020Quote: The following TLR ligands were used at a final concentration of 10 μg/mL to assess TLR responsiveness of bovine monocyte subsets: Pam2CSK4 [C65H126N10O12S,3TFA] (InvivoGen, Labforce, Basel, Switzerland), high-molecular-weight (HMW ...
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bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
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bioRxiv - Immunology 2024Quote: ... low-endotoxin nanoparticles were diluted to 20 μg/mL in buffer and mixed with 1:1 v/v AddaVax adjuvant (InvivoGen vac-adx-10) to obtain a final dose of 1 μg of immunogen per animal ...
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bioRxiv - Microbiology 2024Quote: ... cells were dislodged with a cell scraper and suspended in 10 mL of DMEM with 200 µg/mL Zeocin (InvivoGen catalog # ant-zn), counted on a Beckman Coulter Z2 Particle Counter and Size Analyzer set to a 10-20 µm size range ...