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Citations for Invivogen :
501 - 550 of 587 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The IgJ-GFP construct includes the 1.1kb NdeI/NheI genomic fragment harboring IgJ enhancer activity previously described27 and the 1.4 kb IgJ promoter followed by EGFP gene from pMOD-ZGFPsh vector (Cayla Invivogen), all flanked by two insulators from chicken β-globin gene locus [23] ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were gel purified and cloned into heavy and kappa expression plasmids52,53 adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 ampicillin resistant vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... CASP8-/- × MLKL-/- and subsequently derived gene-deficient BLaER1 macrophages were stimulated with 2 ng/mL LPS from E.coli (Invivogen) for 18 h ...
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bioRxiv - Immunology 2024Quote: ... and IFNL3-like (LOC107521776 or LOC107520938) were obtained by reverse-transcription and PCR from RNA of poly(I:C) (Invivogen) stimulated bat nasal epithelial cells ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
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bioRxiv - Immunology 2023Quote: ... ISGF3 reporter activity was determined from cell culture supernatants using QUANTI-Blue detection reagents according to the manufacturer’s instructions (Invivogen).
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bioRxiv - Immunology 2023Quote: ... Culture supernatants from THP-1 Dual cells (20 µl) were mixed with QUANTI-Luc 4 Lucia/Gaussia reagent (Invivogen), and luminescence was measured by a luminometer ...
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bioRxiv - Immunology 2023Quote: ... was diluted in sterile endotoxin-free water. TLR3 agonist polyinosinic-polycytidylic acid - Poly(I:C) and TLR4 agonist LPS (Lipopolysaccharide from E. coli O55:B5) (Invivogen) were diluted in sterile endotoxin-free physiological water (NaCl 0.9%) ...
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bioRxiv - Immunology 2023Quote: ... Adenosine 5’-triphosphate disodium salt (ATP, CAS: 987-65-5), poly(dA:dT), and nigericin (Nig, CAS: 28643-80-3) were from InvivoGen. Cross linker disuccinimidyl suberate (DSS ...
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bioRxiv - Immunology 2023Quote: ... of filtered PAO1 supernatant or TSB and KGMTM-2 containing purified flagellin at 1μg/ml or vehicle (tlrl-pafla; from P. aeruginosa; InvivoGen) were used for treating hTCEpi cells for various durations as indicated in the figure legends.
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bioRxiv - Microbiology 2023Quote: Heat-killed Salmonella typhimurium (HKSA, tlrl-hkst2), and heat-killed Staphylococcus aureus (HKSA, tlrl-hksa) were purchased from InvivoGen. And the usage of them were following the manufacturer’s instruction.
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bioRxiv - Biochemistry 2023Quote: Jurkat-Lucia TCR-hPD-1 effector cells and Raji-APC-hPD-L1 antigen presenting cells were obtained from Invivogen as components of the PD-1/PD-L1 Bio-IC assay (Invivogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... for all monoclonal cells line and additionally Hygromycin B Gold (0.1 mg/ml) for HEK/PF/5-HT4 (both from Invivogen). Cells of all mentioned lines were grown in 12-well culture plates at 37 °C in a humidified atmosphere of 5% CO2.
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bioRxiv - Immunology 2024Quote: ... NFκB activation was analysed by measuring the luciferase reporter from the cell culture supernatant with QUANTI-Luc reagent (InvivoGen) according to the manufacturer’s instruction using Tecan Infinite (Tecan).
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bioRxiv - Genetics 2024Quote: ... except that the ADP-heptose used in this study was ADP-L,D-heptose (#tlrl-adph-l) from Invivogen. All nucleotide sugars were prepared as 1 mM stock solutions.
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bioRxiv - Microbiology 2022Quote: ... five different pseudotypes were generated using expression plasmids of respective spike variants: for prototype B.1/D614G as before (1) or sourced from Invivogen for VOCs Beta (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Cell Biology 2021Quote: ... The stably transduced population of cells was selected using appropriate antibiotics (5 ug/ml puromycin or 50-100 μg/ml hygromycin Gold, both from Invivogen) for at least 5 days ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43 or 15 µg of SARS-CoV spike protein in 150 µL PBS together with 50 µL Addavax (MF59-like squalene adjuvant from InvivoGen) through intraperitoneally injection (i.p.) ...
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bioRxiv - Immunology 2022Quote: ADCC assays were carried out using CD16-expressing reporter cells from the Jurkat-Lucia NFAT-CD16 cell line (jktl-nfat-cd16, Invivogen) following manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... we supplemented the THP-1 dual cell line with the respective selection agents (100 μg/mL zeocin + 10 μg/mL blasticidin) and the corresponding selection cytostatics from Invivogen.
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bioRxiv - Bioengineering 2022Quote: Non-glycosylated monoclonal human IgG1 antibody against human EGFR (hegfr-mab12, lot: EG12-39-01) and isotype control -Human IgG1 (bgal-mab1, lot: BG1-41-01) were purchased from InvivoGen. Human EGFR (Research Grade Cetuximab Biosimilar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Pathology 2020Quote: ... The conditioned media from each treatment group was analyzed for the presence of SEAP using QUANTIBlue™ detection reagent (Invivogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 10 uL of conditioned media from the co-culture wells were mixed with 50 uL of the commercial Quanti-Luc reagent (Invivogen) in a black ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human antibodies were expressed in HEK-293T cells following transient transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from Invivogen. Recombinant antibodies were purified using Protein A sepharose (IBA ...
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bioRxiv - Physiology 2021Quote: ... Neutralizing mouse anti-human IL-1α and human IL-1β monoclonal IgG1 antibodies and mouse IgG1 isotype control (mabg-hil1a, mabg-hil1b, mabg1-ctrlm) were purchased from InvivoGen and used at the indicated concentration with a 2 h pre-incubation in CM+ prior to addition to FAPs ...
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bioRxiv - Biochemistry 2021Quote: ... Stable MCF10A cells expressing these proteins were generated from MCF10A ARPIN −/− cells 22 transfected as described above and selected with 10 μg/ml Blasticidin (InvivoGen). Individual clones were picked with cloning rings.
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bioRxiv - Microbiology 2020Quote: ... or 7.7G6 mAb were produced in HEK-293T cells following transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from InvivoGen. Human antibodies were purified from cell culture supernatants using Protein-A affinity chromatography ...
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bioRxiv - Immunology 2020Quote: ... the activation of NF-κB drives the expression of the alkaline phosphatase enzyme that induces a color shift from pink to blue of the chromogenic substrate in the HEK-Blue Detection Media (Invivogen). These cells were cultured in complete DMEM medium composed of DMEM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2020Quote: ... TLR4 agonist: CRX-527 (Cat. #tlrl-crx-527) and TLR4 antagonist: LPS-RS Ultrapure (Cat.# tlrl-prslps) were obtained from InvivoGen. Human IL1RL1/ST2 Protein (isoform a ...
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bioRxiv - Immunology 2022Quote: Splenic B cells were isolated from WT and cKO mice and cultured in presence of LPS (InvivoGen, San Diego, CA) 5 µg/ml or 1 µg/ml for 2 ...
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bioRxiv - Immunology 2022Quote: HEK293 stably transfected with an SEAP reporter (HEK-Dual and HEK-Blue) responsive to STAT1 (IFNγ/GAS) or IRF (ISG) were obtained from Invivogen. Cells were seeded in a 96 well plate and treated with IFNγ alone or in the presence of BCL6 inhibitors ...
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bioRxiv - Genetics 2022Quote: ... 20 uL of supernatant from each well was collected and mixed with 50 uL of Quanti-Luc Gold solution (rep-qlcg1, Invivogen) in a white 96-well microplate (655075 ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Neuroscience 2022Quote: ... some cells were activated with LPS (100 ng per mL, triple-purified from E. coli strain O55:B5, InvivoGen, Inc.) or IFNγ (20 ng per mL) ...
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bioRxiv - Immunology 2024Quote: ... 2cz-Tag-it co-culture with RAW 264.7 cells activated for 6 hours with 1.5 ug/ml Ultrapure LPS from E.coli O111:B4 (InvivoGen, Cat#tlrl-3pepls) and 2cz cultured with 50 ng/ml of its strong agonist peptide SIYRYYGL (GenScript ...
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bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
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bioRxiv - Immunology 2024Quote: ... allowing for the observation of cellular responses under the influence of Poly I:C (sourced from Invivogen, San Diego, CA, USA). The knockdown of the Abcf1 gene utilized mouse Abcf1 siRNA ...
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bioRxiv - Immunology 2024Quote: ... and purified by the University of Michigan Center for Structural Biology as previously described.42 Addavax (MF59 similar) was obtained from Invivogen, and the BNT162b2 mRNA vaccine (Pfizer ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatant (20 μL) was then removed from each well and added to 180 μL of QUANTI-Blue detection buffer (InvivoGen). The detection solution was incubated at 37 °C for 0.25-6 h until the lowest standard had visibly turned blue ...
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bioRxiv - Neuroscience 2023Quote: ... The culture media was also supplemented with 0.2% (1:500 dilution from a 5 μg/ml working concentration) Plasmocin™ Prophylactic (ant. mpp; InvivoGen). The day of plating from cryopreservation was allocated as day 0 and 50% media changes were carried out every 2-3 days ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2023Quote: ... 20 µl of media from the THP-1 Dual cells was incubated with 180 µl Quanti-Blue (InvivoGen, # rep-qbs) and incubated for 4 hours at 37°C before quantification at OD635 ...
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bioRxiv - Bioengineering 2023Quote: ... 50 μL cell supernatant was collected from each sample and added to 150 μL of QUANTI-Blue SEAP detection medium (InvivoGen) and incubated for 2 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... with 80 μl of antigen/adjuvant mix containing 10 μg of hM2e or M2ex3 antigen in 40 μl PBS and 40 μl of adjuvant: aluminum phosphate (alum phosphate, AP) or AddaVax (AV) (both AP and AV from InvivoGen). For the hM2e study ...
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bioRxiv - Immunology 2023Quote: ... that are compatible with cloning into the rhesus IgG1 pFUSE expression vectors (pFUSEss-CHIg-rhG1 and pFUSEss-CLIg-rhK from Invivogen; rhIgLC1*01 courtesy of D ...
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bioRxiv - Immunology 2023Quote: ... PBMCs from A3-5 were cultured with 250 ng/mL Poly(I:C)-LMW/LyoVec™ (PolyI:C; Invivogen, San Diego, USA) for 48 h at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... frozen cells were washed twice in complete medium (RPMI with GlutaMAX, 10% FBS, 1 × nonessential amino acids, 1 × sodium pyruvate, 1 × penicillin/streptomycin, 1 × Normocin from InvivoGen), and 2 × 107 cells per ml were suspended in complete RPMI ...