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Citations for Epigentek Group :
101 - 138 of 138 citations for Anti ChAT SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Total histone was isolated from wild type or KDM6A and/or KDM6B deficient U937 cells using EpiQuik total histone isolation kit (Epigentek) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and 2-4 ul of gDNA per sample (approximately 100ng) were analyzed by MethylFlash Global DNA methylation (5-mC) ELISA Easy kit (Epigentek), following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Methylated m6A RNA immunoprecipitation (Me-RIP) was performed according to the protocol of EpiQuikTM CUT&RUN m6A RNA Enrichment Kit (Epigentek). qPCR analysis of the methylated RNA was performed to detect methylated MDA5 mRNA levels.
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bioRxiv - Molecular Biology 2023Quote: ... cells were blocked for 1h in 2% BSA in 0.1% Tween-20 in PBS and then incubated anti-5MC antibody (1:500; A-1014 Epigentek) in 0.1% Tween-20 in PBS overnight at 4°C and subsequently after washing with 1:500 anti-mouse Cy5-conjugated in 0.1% Tween-20 in PBS for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated with primary antibody solutions diluted in blocking buffer including mouse anti-5-mC (1:2000; Epigentek) and rabbit anti-PV (1:1000 ...
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bioRxiv - Physiology 2019Quote: ... Levels of 8-OHdG in RBC DNA was quantified using a competitive immunoassay (300ng DNA, EpiQuick 8-OHdG DNA Damage Quantification Direct Kit Colorimetric, Epigentek, USA) following the manufacturer recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... Fourty-eight hours after irradiation histone proteins were isolated using EpiQuik Total Histone Extraxtion Kit (Epigentek, Farmingdale, NY, Cat #OP-0006). Detailed description of the method is available in SI Appendix.
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bioRxiv - Plant Biology 2020Quote: HDA activity was measured using a commercially available EpigenaseTM HDAC Activity/Inhibition Direct Assay Kit (Epigentek, Cat No. P-4035-48) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: RNA bisulfite treatment and purification of converted RNA was performed using the Methylamp™ RNA Bisulfite Conversion Kit (Epigentek Group Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Global m6A/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY) following the manufacturers’ specifications and using 100 ng as input (in duplicate or triplicate).
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bioRxiv - Biochemistry 2022Quote: ChIP was performed from pancreas of age matched WT and SR-/- mice using EpiQuik tissue chromatin immunoprecipitation kit (Epigentek; #P-2003) as per instructions ...
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bioRxiv - Plant Biology 2022Quote: Global DNA methylation levels were quantified by using 100 ng of genomic DNA from each sample and MethylFlash Methylated DNA Quantification Kit (Colorimetric; Epigentek, NY). This ELISA-based procedure estimates 5-methyl-cytosine fraction in genomic DNA extracts by using specific antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... with gentle sonication and core histone proteins were extracted with the EpiQuik Total Histone Extraction Kit (Epigentek, Cat# OP-0006-100). The extracted lysates and histone proteins were resolved on a 4-20% gradient gel (Biorad ...
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bioRxiv - Biophysics 2021Quote: ... The 5-mC level was analyzed by the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit (Epigentek, P-1030) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative analysis of global 5-mC levels was carried out with the MethylFlash Methylated DNA Quantification Kit (Epigentek, P-1030-48) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: Isolation of genomic DNA was conducted using the FitAmp™ Blood and Cultured Cell DNA Extraction Kit (EpiGentek, Farmingdale, NY, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Input gDNA (100ng) was used as a template to calculate methylation percentage by Methyl flash kit (Cat# P-1030, Epigentek, USA) as described previously [22].
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bioRxiv - Cancer Biology 2022Quote: ... for oxidative DNA damage in DNA samples using the EpiQuik™ 8-OHdG DNA Damage Quantification Direct Kit (Colorimetric) from Epigentek following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... The resulting amplicon libraries were purified using NEBNext® Sample Purification Beads in the kit and a magnetic stand (EpiMag HT 96-Well Magnetic Separator, Epigentek). For pooling ...
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bioRxiv - Biochemistry 2023Quote: ... The collected nuclear extracts (20 μg protein) were then used to measure global DNA methylation and DNMT and TET activities with kits from Epigentek (USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: The m6A methylation level in biotin-RNA was measured using EpiQuik m6A RNA methylation Quantification Colorimetric Kit (Epigentek, Farmingdale, NY, US) according to the manufacturer protocol ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: The m6A level of total RNA was detected using the EpiQuik m6A RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Core histone proteins from E13.5 MGE samples (pooled from 2-4 brains per genotype) were extracted using EpiQuik Total Histone Extraction kit (Epigentek# OP-0006). We obtained 40-80 μg of histone proteins per extraction and loaded 20 μg of protein onto a 4-12% Bis-Tris Plus mini gel (Invitrogen# NW04120BOX) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Here we quantified levels of 8-OHdG in the DNA extracted from embryos using a competitive immunoassay (300 ng DNA, EpiQuick 8-OHdG DNA Damage Quantification Direct Kit Colorimetric, Epigentek, USA) following the manufacturer recommendations ...
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“Long-Term Quarantine is Associated with High Cortisol and Low DNA Methylation in New World monkeys”bioRxiv - Animal Behavior and Cognition 2024Quote: ... The 5 mC methylation content was measured in 100 nanograms of DNA using the MethylFlash Global DNA Methylation ELISA Easy-colorimetric test kit (Cat. No. P1030, Epigentek, USA). Colour change was measured in a plate reader (Varioscan ...
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bioRxiv - Neuroscience 2020Quote: ... pre-blocked with BSA and incubated overnight at 4°C with an anti-Mbd2 (IP grade, Epigentek-A1007, Burlington, ON) antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... 140 mM NaCl 0.05% Triton X-100) was added to the DNA together with 1 µl of anti-5-methylcytosine antibody (A-1014-050, Epigentek) and incubated on a nutator overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Total m6A levels of the extracted RNA were measured using an EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric; Epigentek, New York, USA) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: ... The m6A levels in mRNA were detected by colorimetric ELISA assay with the EpiQuikTM m6A RNA Methylation Quantification kit (Epigentek, P-9005). Measurements were carried out in triplicate for each sample and 5 hearts were used for each group.
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Epigenetic Changes in Saccharomyces cerevisiae Alters the Aromatic Profile in Alcoholic FermentationbioRxiv - Microbiology 2022Quote: ... cerevisiae compared to untreated wild type strain were measured using EpiQuik™ Histone H3 modification multiplex assay kit (Colorimetric; EpiGentek, NY, USA) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Global genomic methylation levels were determined using 100 ng of DNA for each sample using the MethylFlash Methylated DNA Quantification Kit Colorimetric (Epigentek, #P-1034). The absolute methylation level was determined using a standard curve ...
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bioRxiv - Molecular Biology 2019Quote: Global m6A modifications in total organellar RNAs was assessed by the EpiQuik m6A RNA Methylation Quantification Kit (Epigentek Group Inc., Farmingdale, NY) according to the manufacturers’ specifications ...
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bioRxiv - Molecular Biology 2021Quote: ... and 200ng of each sample analyzed for m6A content using the EpiQuik m6A RNA Methylation Quantification ELISA Kit (EpiGentek # P-9005-48). The results were read using a BMG Clariostar plate reader at the Duke Functional Genomics core facility to read the absorbance at 450nm ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin immunoprecipitation in brain of C57 mice at post-natal day 10 (PND 10) and PND 365 was performed using EpiQuik Tissue Chromatin Immunoprecipitation kit (Epigentek #P-2003) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: DNA methyltransferase activities of endogenous DNMT1 and recombinant DNMT1 were measured by EpiQuik DNMT Activity/Inhibition ELISA Easy Kit (P-3139-48, EpiGentek, Farmingdale, NY, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Oxidative DNA damage quantification was carried out using the EpiQuikTM 8-OHdG DNA damage quantification direct kit (Epigentek Group, Inc., Farmingdale, NY USA). This is based on colorimetric quantification of oxidized guanosine residues (8-hydroxy-2-deoxyguanosine ...
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bioRxiv - Molecular Biology 2022Quote: ... The activities of histone deacetylases (HDAC) and histone acetyltransferases (HAT) were analyzed with EpiQuik HDAC Activity/Inhibition Assay Kit (EpiGentek, Farmingdale, New York, USA) and EpiQuik HAT Activity/Inhibition Assay Kit (EpiGentek ...
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bioRxiv - Microbiology 2022Quote: ... Respective cellular fractions (pellet and supernatant) were subjected to histone purification using the EpiQuik™ Total Histones Extraction Kit (Epigentek, OP-0006-100, USA). The purified histone concentration was determined using micro BCA protein assay kit (Pierce ...