Labshake search
Citations for Epigentek Group :
101 - 150 of 185 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... Histones were extracted from cells and tissues using a total histone extraction kit (EpiGentek, Cat. #OP-0006). Before SDS-PAGE ...
-
bioRxiv - Microbiology 2022Quote: ... The cells were then stained with anti-RABV M antibody (A54616; Epigentek; 1:150 dilution) in 0.3% BlockAce in 0.005% Tween-20 in PBS (PBST ...
-
bioRxiv - Microbiology 2021Quote: Quantification of m6A modified RNA was carried out by using EpiQuikTM m6A RNA Methylation Quantification Kit (Colorimetric) (EpiGentek, Farmingdale, NY) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2021Quote: Histones were extracted using the EpiQuick Total Histone Extraction Kit (Epigentek, Farmingdale, NY, USA, cat. # OP-0006) following the manufacturer’s instructions with few modifications ...
-
bioRxiv - Genetics 2021Quote: ... or anti-Cas9 (A-9000-050, Epigentek Group, dilution: 1:2000) were used ...
-
bioRxiv - Genetics 2021Quote: ... Total m6A levels of the extracted RNA were measured using an EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric; Epigentek, New York, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2021Quote: ... we tested 5hmC level by qPCR with BisulPlus™ Loci 5mC & 5hmC Detection PCR Kit (EpiGentek, #P-1067). Briefly ...
-
bioRxiv - Molecular Biology 2021Quote: EpiQuik Colorimetric General Protein-DNA Binding Assay Kit (Epigentek Inc., cat# P-2004-96) was used for measuring DNA binding ability of PNKP WT ...
-
bioRxiv - Molecular Biology 2021Quote: The cytoplasmic and nuclear fractions were isolated with an EpiQuik nuclear extraction kit (EpiGentek, OP-0002) according to the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: The m6A levels of total RNA were measured using an EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric) (Epigentek, Farmingdale, NY) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: RNA bisulfite treatment and purification of converted RNA was performed using the Methylamp™ RNA Bisulfite Conversion Kit (Epigentek Group Inc.) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... DNA was then shipped to a commercial vendor (EpiGentek Group Inc.; Farmingdale, NY, USA) for RRBS as described below.
-
bioRxiv - Molecular Biology 2021Quote: ... and bisulfite conversion was performed with the Methylamp DNA Bisulfite Conversion Kit (Epigentek; catalog #: P-1001). The efficiency of bisulfite-treated DNA was determined by real-time PCR using two pairs of primers where the first pair targeted bisulfite-converted beta-actin (BACT) ...
-
bioRxiv - Molecular Biology 2021Quote: DNMTs activity was measured by EpiQuik DNA Methyltransferase Activity/Inhibition assay kit (Epigentek) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... the tumors were homogenized and the histones were extracted using a Epiquik Total Histone Extraction Kit (Epigentek, Farmingdale, NY, USA). Immunoblotting for these histones was performed under the following conditions ...
-
bioRxiv - Cell Biology 2021Quote: Global m6A/m in total RNA was quantified by the EpiQuik m6A/m RNA Methylation Quantification Kit (Epigentek Group, Farmingdale, NY) following the manufacturers’ specifications and using 100 ng as input (in duplicate or triplicate).
-
bioRxiv - Cancer Biology 2021Quote: ... Chromatin was isolated from 20 million IHH cells or 40mg of of human fatty liver from donor patients (using the EpiQuik Chromatin Immunoprecipitation Kit (Epigentek) according to manufacturer’s instructions for cells or tissues ...
-
bioRxiv - Biophysics 2021Quote: ... The 5-mC level was analyzed by the MethylFlash™ Global DNA Methylation (5-mC) ELISA Easy Kit (Epigentek, P-1030) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Absolute quantification of m6A levels on poly(A) mRNA was determined using Epiquick m6A RNA methylation quantification kit (P-9005-48, EpiGentek) by following the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: Global genomic methylation levels were determined using 100 ng of DNA for each sample using the MethylFlash Methylated DNA Quantification Kit Colorimetric (Epigentek, #P-1034). The absolute methylation level was determined using a standard curve ...
-
bioRxiv - Genomics 2021Quote: ... H3K27me3 (Epigentek ref A-4039, batch 503019) and H4K20me1 (Abcam ref ab9051 ...
-
bioRxiv - Immunology 2021Quote: ... The supernatant was collected (EpiQuik Total Histone Extraction Kit, Epigentek). The protein concentrations were determined using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... m6A immunoprecipitation (MeRIP) was performed using the EpiQuik™ CUT&RUN m6A RNA Enrichment Kit (P-9018, EPIGENTEK, USA) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Quantitative analysis of global 5-mC levels was carried out with the MethylFlash Methylated DNA Quantification Kit (Epigentek, P-1030-48) according to the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protease cleavage (PC) assay buffer (EpiGentek) was used as the assay solutions ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The collected samples were released into 300 μl of protease cleavage (PC) assay buffer (EpiGentek) or NaCl solution at different concentrations by rotating the swab in the buffer for 30 sec ...
-
bioRxiv - Genetics 2021Quote: Extracted DNA (10-200 ng) was bisulphite converted using the Methylamp DNA Modification Kit (#P-1001-2, Epigentek) and eluted in 18 μl elution buffer ...
-
bioRxiv - Microbiology 2021Quote: ... (EpiGentek, NY, USA) with the following settings ...
-
bioRxiv - Molecular Biology 2021Quote: ... EpiQuik DNA methyltransferase activity/inhibition assay kit (Epigentek, USA); ScriptSeq v2 RNA-Seq library preparation kit (Epicentre Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 200ng of each sample analyzed for m6A content using the EpiQuik m6A RNA Methylation Quantification ELISA Kit (EpiGentek # P-9005-48). The results were read using a BMG Clariostar plate reader at the Duke Functional Genomics core facility to read the absorbance at 450nm ...
-
bioRxiv - Immunology 2021Quote: ... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... Protease cleavage (PC) assay buffer (EpiGentek) was used to prepare the furin solutions ...
-
bioRxiv - Immunology 2021Quote: ... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged peptide containing the SARS-CoV-2-specific furin motif (EpiGentek), and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek ...
-
bioRxiv - Immunology 2021Quote: ... anti-rabbit IgG-HRP (EpiGentek) (50 µl ...
-
bioRxiv - Immunology 2021Quote: ... A SARS-CoV-2 neutralization antibody (EpiGentek), which targets the spike RBD ...
-
bioRxiv - Immunology 2021Quote: A peptide (EpiGentek) containing the SARS-CoV-2-specific furin motif and tagged with His and biotin at the N- and C-terminals ...
-
bioRxiv - Neuroscience 2021Quote: ... the amount of m6A that was measured using EpiQuik m6A RNA methylation Quantification Colorimetric Kit (Epigentek, Farmingdale, NY).
-
bioRxiv - Cancer Biology 2021Quote: The EpiQuik In Situ Kit (Cat # P-6001-096, Epigentek, Farmingdale NY) detected the phosphorylation of H2AX at Serine 139 by a colorimetric method measured at 450nm in a 96-well plate where the cells are grown after radiation ...
-
bioRxiv - Cancer Biology 2020Quote: ... Nuclear protein was then isolated from cells using the EpiQuik Nuclear Extraction Kit (EpiGentek Group Inc.), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: Chromatin immunoprecipitation (ChIP) assays were conducted with an EpiQuik Chromatin Immunoprecipitation Kit (Epigentek, Farmingdale, NY, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... a dilution curve ranging from 5 μg to 60 μg was assayed using pooled protein samples from P7 and P90 amygdala to determine the linear portion of the absorption curve along with DNMT positive controls (50 μg/mL; provided by Epigentek) and blanks (containing only assay buffer) ...
-
bioRxiv - Molecular Biology 2020Quote: Total DNMT activity was measured using EpiQuik™ DNA Methyltransferase Activity/Inhibition Assay Kit (P-3001; Epigentek, Farmingdale, NY, USA) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... the dCas9 protein was quantified using the CRISPR/Cas9 assay ELISA kit (Epigentek).
-
bioRxiv - Evolutionary Biology 2020Quote: ... We then sheared samples to approximately 600 base pair (bp) fragments with an Episonic 1100 bioprocessor (EpiGentek; Farmingdale, NY) and assessed fragment length using a High Sensitivity DNA Assay on an Agilent 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... Antibody capture was performed using a ChromaFlash High-Sensitivity ChIP kit (Epigentek) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Genomic DNA (2.4 μg) extracted from cells were bisulfite converted using the Methylamp DNA Modification Kit (Epigentek). The region of interest was PCR amplified in four 50 μL reactions using the following conditions ...
-
bioRxiv - Neuroscience 2020Quote: ... Fourty-eight hours after irradiation histone proteins were isolated using EpiQuik Total Histone Extraxtion Kit (Epigentek, Farmingdale, NY, Cat #OP-0006). Detailed description of the method is available in SI Appendix.
-
bioRxiv - Neuroscience 2020Quote: Global DNA methylation was measured using the Methyl Flash Global DNA Methylation (5mC) ELISA Easy Kit (Colorimetric) (EpiGenteK, Farmingdale, NY), according to manufacturer’s instructions ...
-
The anti-lipidemic drug simvastatin modulates Epigenetic Biomarkers in the Amphipod Gammarus locustabioRxiv - Pharmacology and Toxicology 2020Quote: Global DNA methylation was determined in eight DNA samples per treatment using the MethylFlash Global DNA Methylation (5-mC) ELISA Easy Kit (Colorimetric) from Epigentek® according to the manufacturer’s instructions ...