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Citations for Molecular Devices :
401 - 450 of 460 citations for pIEXBac c EGFP 3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Live-cell imaging was carried out in a temperature (37°C) and CO2-controlled environment using a Micro Confocal high-content imaging system (Molecular Devices, USA) or laser-scanning confocal LSM 880 with airy scan (Zeiss ...
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bioRxiv - Cell Biology 2022Quote: ... Whole-cell current-clamp recording was carried out at room temperature (22 ± 2 °C) using an Axopatch 200B amplifier (Molecular Devices, USA). Pipette (Intracellular ...
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bioRxiv - Developmental Biology 2021Quote: ... Action potentials were recorded at 37°C with the amphotericin-B-perforated patch-clamp technique using a Axopatch 200B Clamp amplifier (Molecular Devices Corporation). Measurements were carried out in Tyrode’s solution containing 140 mmol/L NaCl ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were exposed to the transfection complexes for 24 hours at 37⍰C and 5% CO2 before GFP fluorescence was read on a plate reader (SpectraMax, Molecular Devices, USA). Fluorescence signals were normalized to untreated cells.
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bioRxiv - Microbiology 2020Quote: ... tissue culture plates were imaged under the same conditions (37°C for 72 h in a humidified atmosphere containing 5% CO2) using an ImageXpressXL high content imager (Molecular Devices, UK) with subsequent images processed for phenotype and motility as previously reported [51].
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bioRxiv - Molecular Biology 2023Quote: ... β-gal activity was measured by taking OD420 values every minute at 28°C for 1 hour using a microplate spectrophotometer (Molecular Devices SpectraMax). OD420 readings were normalized using the OD600 values from directly before lysis to give β-gal activity in Miller units (Thibodeau et al ...
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bioRxiv - Microbiology 2024Quote: Microscopy images were acquired at 25°C with a 40X plan Apo objective at selected time points over 48 h on the ImageXpress Micro C® automated microscope (Molecular Devices) in wide field mode ...
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bioRxiv - Neuroscience 2024Quote: ... Whole-cell patchclamp recordings were performed at room temperature (∼20°C) in current- and/or voltage-clamp mode using a Axopatch 200A or Multiclamp 700B amplifiers (Molecular Devices Inc.). Patch pipettes (3-7 MΩ ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were incubated at 37°C for 30 min and absorbance was measured at 562 nm using a microplate spectrophotometer (SpectraMax i3x, Molecular Devices, USA).
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bioRxiv - Developmental Biology 2024Quote: ... Currents were recorded at room temperature (21-23°C) by whole-cell patch clamping using an Axopatch-200B patch clamp amplifier (Molecular Devices, USA)[32 ...
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bioRxiv - Biophysics 2021Quote: ... and currents were recorded with microelectrodes filled with 3 mM KCl (pipette resistance of < 1 MΩ), OC-725C amplifier (Warner, CT, USA) and Digidata 1550 digitizer (Molecular Devices, CA, USA) at 1 kHz with 200 Hz filtering ...
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bioRxiv - Neuroscience 2022Quote: Whole cell patch-clamp recordings were obtained from S1 layer 2/3 neurons with a Multiclamp 700B amplifier (Molecular Devices, San Jose, CA) low-pass filtered at 1.8 kHz with a four-pole Bessel filter ...
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bioRxiv - Neuroscience 2021Quote: ... The assay involved adding 20 µL MTS reagent to each well already containing 100 µL media and incubating the plate for 3 hours before measuring the absorbance at 490 nm with a Spectra Max M2 plate reader (Molecular Devices, Sunnyvale, CA). The background absorbance of MTS in media alone was subtracted from the absorbance obtained in the presence of cells ...
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bioRxiv - Neuroscience 2021Quote: ... Intracellular calcium was detected using a FLIPR calcium 5 no wash assay kit and read on a FlexStation 3 plate reader (Molecular Devices, Sunnyvale USA).
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bioRxiv - Neuroscience 2020Quote: ... Data were digitized at 20 kHz and filtered at 1 or 3 kHz using a Digidata 1440 A system with pClamp 10.2 software (Molecular Devices, Sunnyvale, CA, USA). Only cells with access resistance of <25 MΩ were retained for analysis ...
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bioRxiv - Physiology 2020Quote: ... cells were incubated with Caspase-Glo 3/7 reagent for 30 min and luminescence was measured using a SpectrMax M5 (Molecular Devices, Sunnyvale, CA). Relative luminescence units were normalized to siCon untreated cells and are given as fold increase in caspase 3/7 activity.
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bioRxiv - Neuroscience 2021Quote: ... Data were digitized at 20 kHz and filtered at 1 or 3 kHz using a Digidata 1440 A system with pClamp 10.2 software (Molecular Devices, Sunnyvale, CA, USA). Only cells with access resistance of <25 MΩ were retained for analysis ...
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bioRxiv - Physiology 2022Quote: ... Images were acquired at excitation of 488 nm at the same settings and the fluorescence quantified over 3-4 fields using Metamorph Software (Molecular Devices, Downington PA). For quantitation of the vascular NLRP3 and ICAM-1 ...
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bioRxiv - Immunology 2020Quote: ... and the reaction was stopped with sulfuric acid before measuring the absorbance at 450 nm (A450) in a FlexStation 3 microplate reader (Molecular Devices, Sunnyvale, CA). Non-specific binding to the plate was corrected by the subtraction of the A450 recorded for each chemokine concentration in wells incubated without liposome ...
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bioRxiv - Neuroscience 2022Quote: Whole-cell current-clamp recordings were made from layer 2/3 pyramidal cells with a Multiclamp 200B or 700B amplifier (Molecular Devices, San Diego). Cortical regions and layers were identified using IR video microscopy (Olympus ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 3/7 activity is measured as arbitrary fluorescent unit (AFU) using a SpectraMax Mini plate reader (Molecular Devices, San Jose, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Signals were filtered at 3 KHz and sampled at 10 KHz with a Digidata 1500A analogue-to-digital conversion card (Molecular Devices, Sunnyvale, CA). Mice embryonic cortex neurons cultured in vitro between 7-14 days were recorded as a positive control ...
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bioRxiv - Neuroscience 2023Quote: ... Recordings were made with pClamp 10.3 software using a MultiClamp 700B amplifier set with 4 kHz low-pass Bessel filter and 10 kHz digitization (Molecular Devices, Sunnydale, CA). The light beam of the same laser was directed at the preparation using an 200μm optic fiber submerged into the bath with light beam aimed at the STN ...
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bioRxiv - Microbiology 2024Quote: ... calcein release in 10-fold serial dilutions of liposome samples after incubation with CAB containing 0.5% Triton X-100 was first titrated using a FlexStation 3 microplate reader (Molecular Devices, San Jose, CA). Liposome sample volumes causing a 5 to 10-fold increase in the calcein fluorescent signal (ex = 485 nm ...
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bioRxiv - Immunology 2021Quote: ... incubating for four hours at 37°C, and measuring fluorescence (ex:560nm, em:590nm) on a SpectraMax M5 plate reader (Molecular Devices, Sunnyvale, CA)(27).
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bioRxiv - Cell Biology 2021Quote: ... and the plate was incubated at 37°C with fluorescence measured every 30 minutes for 18 hours by a SpectraMax i3x plate reader (Molecular Devices, Sunnyvale, CA). Fluorescence was plotted versus time ...
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bioRxiv - Microbiology 2021Quote: ... and the reaction at 37 °C was followed by measuring the absorption at 405 nm in a SpectraMax M2 microplate reader (Molecular Devices, Biberach, Germany). Determination of temperature optimum of NamZ was achieved ...
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bioRxiv - Biochemistry 2021Quote: ... Turbidity was monitored at 405 nm (A405) every 30 s for 60 min at 37°C using a SpectraMax 190 (Molecular Devices, Biberach, Germany). In some experiments ...
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bioRxiv - Genetics 2022Quote: HERG currents were recorded at 36±0.1°C with the whole-cell patch-clamp method using an Axopatch 200 B amplifier (Molecular Devices, Sunnyvale, CA, USA). Voltage control ...
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bioRxiv - Biochemistry 2020Quote: ... Hydrolysis was recorded for 180 minutes at 37°C (λex320 nm; λem405nm) using a microplate fluorescence reader (Molecular Devices Spectra Max GEMINI EM). The measurement was performed in triplicates and the initial velocities were determined via the built-in linear regression algorithm ...
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bioRxiv - Cell Biology 2020Quote: ... incubated at 37°C for 4 h protected from light and read by a plate reader (Spectramax i3, Molecular Devices, Sunnyvale, CA, USA) at 585 nm using an excitation wavelength of 555 nm.
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 1 hour at 37°C with 5% CO2 and OD440nm & OD650nm were then measured using a 96-well plate spectrophotometer (Molecular Devices or BioTek). OD650nm was subtracted from OD440nm then percent viability was calculated relative to the no treatment condition.
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bioRxiv - Bioengineering 2023Quote: ... The plate was incubated for 10 min at 37°C before reading on a plate reader (SpectraMax M2, Molecular Devices, San Jose, CA) at 513 nm ...
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bioRxiv - Physiology 2022Quote: ... the supernatant was centrifuged at 900 g for 5 min under 4°C and then measured by a luminometer (SpectraMax Plus384, Molecular Devices, CA, USA).
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bioRxiv - Physiology 2024Quote: All electrophysiological recordings were acquired at room temperature (22–25°C) with an Axopatch 200B amplifier and Digidata 1440 digitizer (Molecular Devices, Sunnyvale, CA). Borosilicate patch pipettes were pulled and polished to resistances of 3-6 MΩ for all experiments using a micropipette puller (model P-97 ...
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bioRxiv - Neuroscience 2023Quote: Slices were transferred to the recording chamber and patch-clamp recordings were made at the temperature of 32-34°C using a Multiclamp 700A (Molecular Devices, Sannyvale, CA). The intracellular solution contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... brain sections immunostained for c-Fos or presynaptic markers were imaged using an ImageXpress Pico automated imaging system (Molecular Devices, San Jose, CA) with a 10× objective (c-Fos ...
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bioRxiv - Cell Biology 2022Quote: ... Fluorescence of C11-BODIPY™ 581/591 was measured by simultaneous acquisition of the green (484/510 nm, oxidized) and red signals (581/610 nm, reduced) with FlexStation® 3 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA), and the ratio of green/red fluorescence was determined as a measure of oxidation of C11-BODIPY™ 581/591.
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bioRxiv - Pathology 2020Quote: ... and incubated at room temperature for 10 min before reading in a FlexStation 3 Benchtop Multi-Mode Microplate Reader (Molecular Devices, San Jose, California) (250-ms integration).
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bioRxiv - Neuroscience 2023Quote: ... Recordings were done by impaling body-wall muscle 6-7 in abdominal segment 3 with a 20-25 MÙ electrode and were amplified using an Axoclamp 900A amplifier (Molecular Devices, San Jose, CA). Recordings were exported to a computer using an Axon Digidata 1550B (Molecular Devices ...
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bioRxiv - Molecular Biology 2024Quote: ... luciferase detection reagent was added to each well 10 min prior to luminescent determination with a FlexStation 3 Multimode Microplate Reader (Molecular Devices, San Jose, CA). In the absence of a known MNRR1-inducing small molecule ...
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bioRxiv - Neuroscience 2024Quote: ... Whole-cell patch recordings from pyramidal neurons (PyNs) in layer 2/3 of the medial prefrontal cortex (mPFC) were performed using Axopatch 700B amplifiers (Molecular Devices, Union City, CA). Slices were visualized using an Olympus Bx51 microscope equipped with infrared-differential interference contrast optics (IR-DIC ...
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bioRxiv - Biophysics 2021Quote: ... Photocurrents were recorded 48-72 h after transfection in whole-cell voltage clamp mode at room temperature (25°C) with an Axopatch 200B amplifier (Molecular Devices, Union City, CA) and digitized with a Digidata 1440A using pClamp 10 software (both from Molecular Devices) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were transferred to aCSF solution for 30 minutes at 35 °C and current clamp whole-cell recordings were performed using a Multiclamp 700A amplifier (Molecular Devices, Palo Alto, USA). Signals were filtered online at 10 kHz and digitized at 40 kHz and traces stored on the computer using Signal 2 software (Cambridge Electronic Design ...
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bioRxiv - Neuroscience 2020Quote: Whole-cell current clamp experiments were performed at near physiological temperature (32-34 °C) using an Axon Multiclamp 700B amplifier (Molecular Devices, Sunnydale, CA, US). For morphological reconstruction and characterization ...
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bioRxiv - Cancer Biology 2023Quote: ... The experiments were conducted at room temperature (21°C) using the whole-cell patch-clamp technique with an Axopatch 200B amplifier (Molecular Devices, Sunnyvale, CA, USA). The voltage protocol consisted of a holding potential of −60 mV followed by voltage steps from −100 to +160 mV in 20 mV increments lasting 0.5 s ...
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bioRxiv - Cell Biology 2022Quote: ... CM-DCF fluorescence inside cells was measured at 485 nm for excitation and 538 nm for emission with FlexStation® 3 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). The amount of emitted fluorescence was correlated to the quantity of reactive oxygen species (ROS) ...
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bioRxiv - Bioengineering 2022Quote: ... was normalized to bacterial growth using optical density measured as absorbance at 600 nm (OD600) on a plate reader (Spectra Max 3, Molecular Devices, San Jose, CA, USA). Bioluminescent signals were quantified using the 8x12 grid ROI for all wells (in vitro thermocycler induction ...
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bioRxiv - Microbiology 2021Quote: ... The plates were then incubated for 3 min at room temperature and the luminescence was assessed using a plate luminometer (Molecular Devices, San Jose, CA, USA) with 1 sec integration time ...
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bioRxiv - Plant Biology 2023Quote: ... The plate was cooled for 3 min at room temperature before measuring the absorbance with a SpectroMax M5 UV/Vis spectrophotometer (Molecular Devices, San Jose, CA, USA) at 630 nm ...