Labshake search
Citations for Biotium Inc. :
1 - 50 of 93 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pathology 2021Quote: ... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium).
-
bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
-
bioRxiv - Genetics 2019Quote: ... quantitative PCR (RT-qPCR) was performed on cDNA (described above) using Eva Green (Biotium) on a Step-One Plus Real time PCR system (Applied Biosystems) ...
-
bioRxiv - Bioengineering 2024Quote: ... A typical reaction contained 100 nM of each primer and either 150 nM fluorescent probe or lx EvaGreen dye (Biotium, Fremont, CA, USA).
-
bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The primers (Microsynth, Balgach) were used in the Real-Time PCR reaction with Fast EvaGreen® qPCR Master Mix (Biotium, USA) on ABI StepOnePlus (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
-
bioRxiv - Genetics 2021Quote: ... Cells were incubated with 150 nM TMRE (Biotium, 70005) for 15 minutes ...
-
bioRxiv - Neuroscience 2022Quote: ... then nuclei were stained with RedDot2 (Biotium, #40061, 1:150 dilution) in PBS containing 500 mM NaCl at 37°C for overnight ...
-
bioRxiv - Neuroscience 2022Quote: ... samples were incubated in a nuclear staining solution (1:150 dilution of RedDot, Biotium 40061-T) in PBS with 500 mM NaCl ...
-
bioRxiv - Bioengineering 2022Quote: ... dams were injected intraperitoneally with d-luciferin with potassium salt (150 mg/kg) (Biotium, Fremont, CA). Anesthetized dams were placed supine into the IVIS Lumina III (PerkinElmer ...
-
bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
-
bioRxiv - Microbiology 2023Quote: To differentiate between viable and non-viable cells and viruses in qPCR, half of the aliquots mentioned above (i.e., the second set of triplicates) received a PMAxx dye treatment (Biotium, Fremont, CA). Aliquots of the biomass samples (230 μL ...
-
bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
-
bioRxiv - Genomics 2019Quote: ... according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium) and 1x ROX reference dye (Invitrogen ...
-
bioRxiv - Bioengineering 2022Quote: ... Following treatment cells were resuspended in 100 µL of 150 mM NaCl and stained using a viability/cytotoxicity assay kit for live and dead bacteria (Biotium, #30027) according to the manufacturers protocol ...
-
bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were visualised with GelRed (Biotium, USA) gel electrophoresis and gel-purified ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized with GelRed dye (Biotium) after agarose gel electrophoresis using a GelDoc imaging system (BioRad).
-
bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
-
bioRxiv - Genomics 2022Quote: A PMAxx Real-Time PCR Bacterial Viability Test (Biotium Inc.), designed for selective detection of viable S ...
-
bioRxiv - Developmental Biology 2021Quote: ... and qPCR was performed using Eva Green PCR Master Mix (Biotium) from nine regenerating fragments in four (Figure 4.2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium) in a 1X TAE buffer and subsequently captured the gel images using G:Box EF2 (Syngene ...
-
bioRxiv - Neuroscience 2020Quote: ... Slides were allowed to cure protected from light at RT for at least one day before the short edges of the coverglass only were sealed with CoverGrip (Biotium #23005, Fremont, CA, USA).
-
bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
-
bioRxiv - Molecular Biology 2020Quote: ... PCR products were visualized by electrophoresis on agarose gel containing GelRed (Biotium) and band sizes were evaluated against a 100 bp molecular weight marker (NEB) ...
-
bioRxiv - Molecular Biology 2019Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
-
bioRxiv - Molecular Biology 2022Quote: PCR products were mixed with 1x GelRed prestain loading buffer (Biotium #41010), separated on a 2% or 4% agarose gel (Seakem LE agarose ...
-
bioRxiv - Immunology 2021Quote: ... PCR products were run on 1% agarose gel and visualised using GelRed (Biotium) and transgene sequence confirmed by Sanger sequencing at the Australian Genome Research Foundation (AGRF).
-
bioRxiv - Molecular Biology 2020Quote: ... PCR products were run on a 1.2% agarose gel containing GelRed™ (Biotium). After electrophoresis only a single band was observed ...
-
bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
-
bioRxiv - Plant Biology 2023Quote: ... PCR products were analyzed on a 1 % agarose gel containing GelRed (0.01%, Biotium) and visualized under UV light.
-
bioRxiv - Genetics 2023Quote: ... The PCR products were resolved on a 1.5% agarose gel containing GelRed (Biotium) and visualized under UV light to distinguish expanded from non-expanded CAG repeats ...
-
bioRxiv - Microbiology 2020Quote: ... PCR products were resolved on 1.0% agarose gel containing GelRed (Biotium, diluted 1:10,000) and imaged on a ChemiDoc MP Imaging System (Bio-Rad).
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed in 10 µL reactions with EvaGreen Mastermix (Biotium) using kit-specified reaction parameters in a Quant Studio 5 Real-Time PCR machine (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
-
bioRxiv - Zoology 2019Quote: ... PCR fragments were size-separated in 1.5% agarose gels and visualized with GelRed (Biotium). The resulting banding pattern of X (~975 bp ...
-
bioRxiv - Genetics 2021Quote: ... Quantitative PCR (qPCR) was preformed using the Forget-Me-Not qPCR Master Mix (Biotium) with a BioRad iCycler thermocycler ...
-
bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...
-
bioRxiv - Molecular Biology 2019Quote: ... according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium, Hayward, USA), 1 µl of cell culture ...
-
bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by electrophoresis using a 2% agarose gel containing GelRed (Biotium) and visualized using an Azure c400 imager (Azure Biosystems) ...
-
bioRxiv - Zoology 2022Quote: All PCR products were verified on a 2% agarose gel stained with GelRed (Biotium). From each of the 12 libraries (2*3 replicates for MiFish-U and 2*3 replicates for Mamm01 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCR products were run and visualized on a 1.5% agarose gel using gelgreen (BIOTIUM). To reduce the number of samples for subsequent purification ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... PCR amplification was verified by visualizing on a 1 % agarose gel with GelRed (Biotium) and 1 kb Hyperladder (Bioline) ...
-
bioRxiv - Plant Biology 2020Quote: ... PCR products were run in 1.5% agarose gel electrophoresis stained with GelRed (Biotium, Hayward, USA).
-
bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was conducted using non-specific DNA-binding dye EvaGreen (Biotium, #31000) to quantitate the total amount of amplification products ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were visualized on agarose gels (1%) stained with Gel Red (Biotium Inc., USA). The PCR products were sequenced using BigDye Terminator v3.1 cycle sequencing kit and cleaned with BigDye XTerminator purification kit (Applied Biosystems ...
-
bioRxiv - Immunology 2023Quote: ... PCR products were visualized on a 1% agarose gel with GelRed Nucleic Acid Stain (Biotium). PCR products were purified using Nucleospin Gel and PCR Clean-up kit (Takara) ...
-
CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...