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Citations for Biotium Inc. :
101 - 150 of 167 citations for Zinc Finger With KRAB And SCAN Domains 5 ZKSCAN5 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... The larvae were housed in 10 μM INK128 (or DMSO) containing water and at 36 hpa they were treated with 5 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Biotium, #10058) for 55 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed and incubated for 5 minutes at 37°C with 1X Pre-Staining Solution (MemBrite® Fix 543/560, #30094, Biotium) diluted in DPBS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Developmental Biology 2019Quote: ... Secondary antibodies: pFAK – Goat anti rabbit IgG (H+L) (CM405F, Biotium, Fremont, CA, USA), AQP-1 – F(ab’)2-Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Genetics 2019Quote: ... and Alexa-Fluor 488-labeled anti-rabbit secondary antibody (#20012, Biotium, Inc., Fremont, CA) at a 1:5000 and 1:1000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... conjugated to CF555 (Mix-n-Stain CF Antibody Labeling Kit, Biotium, Fremont, CA, USA)
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bioRxiv - Immunology 2024Quote: Sections were stained with fluorophore-conjugated antibodies and Nucspot 750/780 nuclear stain (Biotium) overnight at room temperature and coverslipped with Fluoromount G mounting media (Southern Biotec) ...
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bioRxiv - Genetics 2021Quote: The quality of DNA was evaluated by electrophoresis using 0.8 % (w/v) agarose gels stained with 5 μL/100 ml Gel Red® (Biotium Inc., USA) to enable visualization ...
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bioRxiv - Neuroscience 2023Quote: DRG neurons grown on coverslips as described above were first treated with a blocking solution consisting of sterile HBSS with 10 mM HEPES + 1% horse serum for 5 minutes at room temperature followed by staining with HBSS/HEPES + 70 mM Di-8-ANEPPS (Biotium, Fremont, CA) for 15 minutes room temperature and recovery in HBSS/HEPES + 10% horse serum for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: Secondary antibodies used: anti-mouse IgG (donkey, CF568-conjugated, Biotium, 20105, AB_10557030, dilution 1:500), anti-chicken IgY (goat ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated in PBS containing secondary antibodies CF543 donkey anti-rabbit (1:500, Biotium) and CF633 donkey anti-goat (1:500 ...
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bioRxiv - Immunology 2020Quote: ... and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium, Fremont, CA, USA), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... CF 555 donkey anti-rabbit IgG (H+L) 555 secondary antibody (Biotium #20038, 1:500), CF633 donkey anti-mouse IgG (H+L ...
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bioRxiv - Genetics 2023Quote: ... and stained with Annexin V-APC antibody and DAPI (0.1 μg/ml, Biotium, BT-40043) using the Annexin V Apoptosis Detection Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... we utilized the anti-CD44 mouse monoclonal antibody (CF488A) [clone: HCAM/918] (Biotium, BNC880918-100) with affinipure Fab fragments goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with secondary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 1 hour at RT ...
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bioRxiv - Genomics 2019Quote: ... RNA integrity was evaluated by electrophoresis in denaturing agarose gel (1% agarose, 5% formamide, 1X TAE) stained with 3x Gel Red (Biotium Inc., CA, USA). The RNA was quantified using the Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Calcium transients of cultured embryonic cardiomyocytes at DIV4-5 were measured after incubating myocytes with the calcium-sensitive fluorescence dye Fura2-AM (1 μM, Biotium, Cat: 50033-1) diluted in 0.02% Pluronic acid F-127/DMSO (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then washed once with NE solution and incubated in 5 µg/mL wheat germ agglutinin conjugated to Alexa Fluor 405 (Biotium cat#29028-1) diluted in NE solution for 2.5 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... CF680- and CF568-labeled secondary antibodies were prepared through reactions of the corresponding succinimidyl esters (Biotium) with secondary antibodies from Jackson ImmunoResearch.
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used: Donkey CF-488A anti-rat IgG (Biotium 20027, 1:000) and CF-568 anti-rabbit IgG (Biotium 20698 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were CF680 goat anti-mouse IgG (Biotium, cat no: 20065; dilution 1:15000) and CF770 goat anti-rabbit IgG (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed in PBS and incubated with the following secondary antibodies (1:500, Biotium) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were Donkey anti-Goat IgG conjugated with CF488 (for GFP, 1:1000, #20016-1, Biotium), Donkey anti-Rabbit IgG conjugated with CF594 (for pERK ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated with secondary antibodies donkey anti-rat 568 (BIOTIUM, Cat. No. 20092, 1:1000), donkey anti-rabbit 647 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Dye conjugation was performed using the Mix-n-Stain CF405M Antibody Labeling Kit (Biotium, Fremont, CA USA). Anti-TERT antibody conjugated with CF405M was diluted at 1:5 in storage buffer.
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bioRxiv - Neuroscience 2020Quote: Retinal sections immunolabeled with α-Rb MCT2 primary antibody were incubated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium) for thirty minutes after blocking and before primary antibody incubation ...
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bioRxiv - Bioengineering 2022Quote: ... was fluorescently conjugated in-house with an amine-reactive fluorescent dye antibody conjugation kit (Biotium, Fremont, CA) and used as a detection label.
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bioRxiv - Cancer Biology 2023Quote: ... tissues were incubated with secondary antibodies coupled with fluorescent dye (dilution 1/800, Biotium, Fremont, CA, USA) according to the species in which the primary antibodies were produced for 3h at RT and with or without rhodamine-phalloidin (dilution 1/200 ...
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bioRxiv - Biochemistry 2021Quote: ... Goat Anti-Rabbit (Cat. #89138-520) and anti-mouse CF680 (Cat. # 20067) secondary antibody were purchased from Biotium. Goat anti-rabbit DyLight 800 secondary antibody was purchased from Thermo Fischer Scientific (Cat ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... organs were incubated as above with Alexa Fluor 488 secondary antibody and 0.4 U/ml CF568 phalloidin (Cat#00044, Biotium). Tissue was washed three times in PBS and mounted on a glass slide in ∼50 µl of Vectashield and covered with a #1.5 thickness 22 x 22 mm cover glass (Cat#2850-22 ...
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bioRxiv - Molecular Biology 2019Quote: ... APC-conjugated normal mouse IgG was produced using the Mix-n-Stain™ APC Antibody Labeling kit from Biotium Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... CAR antibodies were conjugated with the CF647 fluorophore (CAR-647) using the Mix-n-Stain antibody labeling kit according to the manufacturer’s protocol (Biotium). Sections were processed first with rabbit polyclonal Cav1.4 or EAAT2 antibodies and corresponding secondary antibodies as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting signals were detected by incubation with secondary antibodies conjugated to fluorophores (Thermo Fisher Scientific, Biotium, or Jackson ImmunoResearch). Sections were counterstained with DAPI ...
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG, 1:1000, Biotium; CF594-conjugated donkey anti-guinea pig IgG ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... FGOs were incubated with anti-rabbit IgG (H+L) CF 488A and anti-mouse IgG (H+L) CF 594 secondary antibodies (Biotium) (dilution 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Developmental Biology 2020Quote: ... was conjugated to Alexa 647 using the Biotium Mix-n-Stain R-PE(PE) Antibody Labeling kit (Biotium, cat no: 92298) following the manufacturers protocol ...