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Citations for Biotium Inc. :
101 - 150 of 168 citations for Trypan Blue Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... At harvest samples were treated with Live-or-Dye™ Fixable Viability Stain (Biotium) at a 1:1000 dilution in 1X PBS/1% FBS for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... cells were first stained with Live-or-Dye™ 405/545 (Biotium, Fremont, CA) followed by staining with PE anti-human CD69 (clone ...
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bioRxiv - Immunology 2020Quote: ... and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium, Fremont, CA, USA), respectively ...
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bioRxiv - Immunology 2022Quote: ... Tumor cells were labeled with CellBrite® Green membrane dye (Biotium Inc, Fremont, CA, USA) (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative real-time PCR was conducted using non-specific DNA-binding dye EvaGreen (Biotium, #31000) to quantitate the total amount of amplification products ...
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bioRxiv - Physiology 2024Quote: ... cells were loaded with 1 μM Ca2+-specific dye Rhod2-AM (50024, Biotium, Fremont, CA) that preferentially localizes in mitochondria ...
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bioRxiv - Cell Biology 2020Quote: Mitochondrial membrane potential was assessed in live cells using the fluorescent dye tetramethylrhodamine (TMRM; Biotium 70017). Briefly ...
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bioRxiv - Physiology 2021Quote: ... [Ca2+]m was measured in cells loaded with Ca2+ specific dye Rhod2-AM (Biotium, Cat #50024) (1 μM ...
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bioRxiv - Cancer Biology 2020Quote: Cellular death was measured with Live-or-Dye™ 488/515 Fixable Staining Kit (Biotium, USA) as per as manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Isolated RNA was separated on a 1% agarose gel (1XTBE) and visualized using GelRed dye (Biotium). Both inputs (corresponds to 5% ...
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bioRxiv - Cell Biology 2020Quote: ... PCNA antibody was pre-labeled using a Mix-n-StainCF-568 Dye Antibody Labeling Kit (Biotium; 92275) accordingly to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: ... primary antibodies were directly conjugated to fluorophores using Mix-n-Stain CF Dye Antibody Labeling Kits (Biotium) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).
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bioRxiv - Cell Biology 2022Quote: ... Dye conjugation was performed using the Mix-n-Stain CF405M Antibody Labeling Kit (Biotium, Fremont, CA USA). Anti-TERT antibody conjugated with CF405M was diluted at 1:5 in storage buffer.
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bioRxiv - Bioengineering 2022Quote: ... was fluorescently conjugated in-house with an amine-reactive fluorescent dye antibody conjugation kit (Biotium, Fremont, CA) and used as a detection label.
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bioRxiv - Cancer Biology 2023Quote: ... tissues were incubated with secondary antibodies coupled with fluorescent dye (dilution 1/800, Biotium, Fremont, CA, USA) according to the species in which the primary antibodies were produced for 3h at RT and with or without rhodamine-phalloidin (dilution 1/200 ...
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bioRxiv - Physiology 2023Quote: ... and voltage (Vm)-sensitive dyes (RH237, 50 µL of 1 mg/mL in DMSO, Biotium, Hayward, CA) through the coronary perfusion ...
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies were first labelled with fluorescent dyes using the Mix-n-Stain antibody labelling kit (Biotium) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: Samples were prepared by mixing SEC-purified CD4-Ig with 25X GloMelt™ Dye (Biotium, #99843-20uL) to reach a final concentration of 10 nM CD4-Ig and 2X dye ...
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bioRxiv - Genetics 2021Quote: ... and an aliquot was used to verify size on an agarose gel stained with GelGreen dye (41005, Biotium). Final libraries underwent quality control verifications using a bioanalyzer and were sequenced on an Illumina NextSeq500.
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bioRxiv - Cell Biology 2021Quote: ... lenses were placed in staining solution containing fluorescent CF640 dye conjugated to wheat-germ agglutinin (Biotium, Fremont, CA) (1:500) ...
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bioRxiv - Microbiology 2022Quote: ... The viability of the bacteria was determined using qPCR after PMAxx™ Dye treatment (Biotium San Francisco, US) allowing specific DNA amplification of viable bacteria only ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Plant Biology 2021Quote: ... Four μL of each reaction product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Genomics 2019Quote: ... according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium) and 1x ROX reference dye (Invitrogen ...
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bioRxiv - Immunology 2021Quote: Apoptotic DNA fragmentation was examined using 30064 CF™ 594 Dye TUNEL Assay Apoptosis Detection Kit (Biotium, Cedarlane) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The success of amplification was monitored by 10% polyacrylamide gel electrophoresis and staining with GelGreen DNA dye (Biotium).
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bioRxiv - Neuroscience 2023Quote: ... their positivity for RedDot1 (Viability dye, Far-red DNA intercalating agent, Biotium, #40060-1, λEx/λEm (with DNA) = 662/694 nm) ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
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bioRxiv - Neuroscience 2024Quote: ... and the excess AM1-43 dye was neutralized using a quenching solution composed of 0.2 mM SCAS (Biotium) in L-15 ...
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bioRxiv - Genetics 2022Quote: ... we performed PCR in 10 μl reactions using Apex Taq DNA polymerase and EvaGreen Dye (Biotium, Fremont, CA, USA) as the intercalating dye ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Microbiology 2022Quote: ... Four μL of the ITS PCR product was mixed with 1 μL of loading dye containing Gel Red (Biotium), and visualized on a 1% agarose gel after 60 minutes at 100 V ...
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bioRxiv - Microbiology 2020Quote: ... wherein biotin-Ahx-RPARPAR-OH (RPARPAR; TAG Copenhagen A/S) was used as the targeting moiety and CF555 dye (#92130, Biotium) or CF647 (#92135 ...
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... then maintained in complete darkness for two days prior to incubation with a cell viability stain (Live-or-Dye 488/515 (Biotium). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... A paired sample design was used where one DNA extraction tube was treated with the chemical propidium monoazide (PMAxx Dye, 20mM in H2O from Biotium) which prevents amplification of exDNA ...
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bioRxiv - Cell Biology 2019Quote: ... freshly thawed cells were diluted at 1×106 cells/mL and CellBrite TM NIR790 Cytoplasmic Membrane Dye (Biotium, cat.30079) was added at the concentration of 1µM and incubated at 37°C for 20 minutes with frequent agitation ...
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bioRxiv - Genetics 2020Quote: ... Samples were pulse centrifuged up to 1200 rpm and split in two, where one part (n = 230, PMA treated samples) was added PMA dye (Biotium, USA to a final concentration of 50 µM ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Developmental Biology 2023Quote: ... to obtain single-cell suspensions.68,86 Cells were stained with a far-red cell membrane-permeant nuclear dye (RedDot™1, Biotium) to specifically select live cells and DAPI to exclude dead cells ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions of each of the cell lines were separately labeled with either one or both of ViaFluor 488 SE and ViaFluor 405 SE fluorescent dyes (Biotium) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cell growth quantification was performed using the ViaFluor 488 SE cell proliferation dye in minCMV reporter HEK293T cells (Biotium 30086) or CellTrace Far-Red proliferation dye in pEF reporter TC28a2 cells (Thermo C34564 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Bioengineering 2024Quote: ... Samples on day 7 post-inoculum were incubated with PMA before being treated with a blue LED photolysis device (Biotium, San Francisco), as described above ...
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bioRxiv - Genetics 2021Quote: ... The size selected libraries were verified for quality control by bioanalyzer as well as verification of the library size via visualization on an agarose gel stained with GelGreen dye (Biotium, 41005). Libraries were then sequenced on NovaSeq6000 Sprime Paired End (PE ...
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bioRxiv - Genetics 2019Quote: ... The number of amplification PCR cycles for each sample was determined individually by performing a qPRC reaction of 7.5 µl aliquote of the mix with an addition of the EvaGreen dye (Biotium, Fremont, U.S.). The amplified libraries were SPRI purified (upper cut 0.5x ...
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...