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Citations for Biotium Inc. :
51 - 100 of 239 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
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bioRxiv - Neuroscience 2021Quote: ... Samples separated on 20% PAGE gels were stained using PAGE GelRed Nucleic Acid Gel Stain (Biotium). Homemade gels using standard agarose or MetaPhor Agarose were stained using GelRed ...
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bioRxiv - Genetics 2024Quote: ... Amplification success was verified on a 0.8% agarose gel stained with GelRed Nucleic Acid Gel (Biotium). After verification ...
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bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 u ml−1 phalloidin CF555 (Biotium, 00040) were added together with the secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibodies and secondary antibodies conjugated to CF568 (1:3000, Biotium). A few additional modifications were included to improve the specificity of labeling for dSTORM ...
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bioRxiv - Biochemistry 2022Quote: ... RNA integrity was evaluated by electrophoresis on 1 % agarose gel using the GelGreen Nucleic Acid Stain (Biotium) dye following the manufacturer’s instructions.
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bioRxiv - Zoology 2020Quote: ... PCR success was checked on a standard 2% agarose gel visualized with GelRed nucleic acid stain (Biotium). Sequencing of successful PCR product was performed by the Psomagen Inc ...
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bioRxiv - Microbiology 2022Quote: ... DNA was separated by electrophoresis on a 0.8% agarose gel containing GelRed nucleic acid gel stain (Biotium) in Tris-acetate-EDTA buffer (120 V for 40 minutes) ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 hr at room temperature with 5% non-fat dry milk (Apex #20-241) or 5% BSA (Biotium #22013) in TBST (10 mM Tris ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... incubated in secondary antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 and 0.01% SDS for 1 hr ...
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bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were visualized after gel electrophoresis and ultrasensitive GelRed® nucleic acid gel stain (Biotium, Fremont, CA, USA) on a UV-light transilluminator ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Amplified fragments were visualized in 2% agarose gels stained by GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA). While amplification of short fragments (~ 570 bp ...
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bioRxiv - Developmental Biology 2020Quote: ... separated on a 1% agarose gel and visualized with GelRed nucleic acid stain (cat #41001, Biotium, Fremont, CA) using a Syngene G:Box Chemi imager (Syngene ...
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bioRxiv - Genetics 2019Quote: ... PCR amplicons were visualized via gel electrophoresis with GelRed® Nucleic Acid Gel Stain (Biotium, Fremont, California, US) and processed with the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... and DAPI (5 μg/ml) (Biotium, Cat #BT-40043). Staining with DAPI was done for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μg/ml of DAPI (Biotium; Cat #BT-40043) was used as a viability dye ...
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bioRxiv - Immunology 2023Quote: ... and NucView-488 at 5 μM (Biotium, Cat. 10402). NucView-488 was selected to visualize activation of Caspase-3 in apoptosis ...
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bioRxiv - Microbiology 2019Quote: ... The resulting PCR products were separated on 1% agarose gel and visualized using GelRed Nucleic Acid Gel Stain (Biotium) under UV illumination in GelDoc-It system (UVP) ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were then run on a 1% agarose gel and visualised using GelRed Nucleic Acid Gel Stain (Biotium). Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... and the RT-PCR products were analysed by standard agarose gel electrophoresis visualized by GelRed Nucleic Acid Stain (Biotium) and subjected to Sanger sequencing (core facility of the Haartman Institute ...
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bioRxiv - Molecular Biology 2022Quote: ... and 11 µL H2O and run on a 1.2% agarose gel containing 1X GelGreen Nucleic Acid Stain (Biotium 41005) at 130 V for 1.5 hours ...
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bioRxiv - Genomics 2024Quote: ... and the integrity was assessed by agarose gel electrophoresis (1% wt/vol agarose in Tris-acetate-EDTA) amended with 1:20,000 (v/v) nucleic acid stain GelRed (Biotium). The isolates were confirmed as A ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR products (∼ 700 bp) were loaded into 1.5 % agarose containing GelRed Nucleic Acid Gel Stain 10,000X (Biotium®) and electrophoresed in 0.5 × TBE buffer for 1 h at 3 V/cm ...
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bioRxiv - Neuroscience 2020Quote: ... applying 5 μl of Fluorogold (Hydroxystilbamine FluoroGold, Biotium, Fremont, CA) to the area ...
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bioRxiv - Synthetic Biology 2021Quote: ... fluorescent secondary antibodies from Biotium, cat# 20308 ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were analyzed via agarose gel electrophoresis on a 1 % (w/v) agarose gel with 0.001 % GelGreen® Nucleic Acid Gel Dye (Biotium) (90 V ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were separated by electrophoresis in a 1 % TAE-agarose gel stained with GelRed nucleic acid gel stain (Biotium), gel-purified (QIAquick gel purification Kit ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Microbiology 2019Quote: ... An aliquot of the T7 transcription reaction (before and after purification) was run in a 2.0% agarose gel using GelRed® Nucleic Acid Gel Stain (Biotium) as above to confirm the production of dsRNA ...
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bioRxiv - Immunology 2021Quote: ... Second round PCR products were subjected to electrophoresis on a 1.5% agarose gel containing 0.1% Gel Red Nucleic Acid Stain (Biotium, Cat#: 41002). Positive wells were then purified using either ExoSAP-IT (Affymetrix ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4ul of PCR product was visualised by electrophoresis on a 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium).
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bioRxiv - Evolutionary Biology 2021Quote: ... All reactions were visualized in a 1.5% Agarose gel containing 1X GelRed fluorescent nucleic acid dye (Biotium, Fremont, CA, USA). Positive samples were sequenced on an Applied Biosystems 3730XL DNA Analyzer at the University of Arizona Genetics Core.
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were analyzed on 2% agarose gel and visualized with the aid of GelGreen nucleic acid gel stain (Biotium).
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bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies included: CF 488- or CF 594-conjugated anti-goat antibody (Biotium Inc., Hayward, CA), CF 647-conjugated anti-rabbit antibody (Biotium) ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were separated by electrophoresis on 1% agarose gel and visualized by staining with GelRed™ Nucleic Acid Gel Stain (Biotium). Nested PCR products were pooled and purified using Monofas® DNA purification Kit I (GL Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Plant Biology 2023Quote: ... Amplicons products were analyzed by electrophoresis on a 1% agarose gel in Tris-acetate-EDTA (TAE) buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Plant Biology 2022Quote: ... PCR products were visualized by 1.5% agarose gel electrophoresis with GelRed staining (GelRed® Nucleic Acid Gel Stain, Biotium, Fremont, USA) and UV illumination ...
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bioRxiv - Genomics 2023Quote: ... PCR products were analyzed by electrophoresis on a 1% agarose gel in TAE buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Cell Biology 2020Quote: ... PCNA antibody was pre-labeled using a Mix-n-StainCF-568 Dye Antibody Labeling Kit (Biotium; 92275) accordingly to the manufacturer protocol.
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Cell Biology 2022Quote: ... primary antibodies were directly conjugated to fluorophores using Mix-n-Stain CF Dye Antibody Labeling Kits (Biotium) according to the manufacturer’s instructions.