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Citations for Biotium Inc. :
351 - 370 of 370 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Equal volumes of PCR reactions were run on a 1% agarose gel and visualized with GelRed Nucleic Acid Stain (Biotium, Fremont, CA).
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were washed three times with DPBS, and the solution of To-pro3 (Thermo Fischer Scientific, T3605, 1:1000) or Hoechst (Biotium, 33342, 5μM) was added and incubated for 30 min at RT in the dark to stain the nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells stably expressing mt-AEQ were harvested at a density of 500,000 cells/mL in growth medium supplemented with 20 mM HEPES (pH 7.4/NaOH) and the aequorin was reconstituted by incubation with 2 µM native coelenterazine n (Biotium, BOT-10115-1) for 2.5 h at RT ...
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bioRxiv - Microbiology 2019Quote: ... Gel electrophoreses of PCR products were done using 1% Agarose gel matrix with the addition of 10,000X GelRed™ Nucleic Acid Gel Stain (Biotium, Inc. CA, USA). Samples were run alongside 100bp exACTGene™ DNA ladder (Fisher BioReagents™ ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Genomics 2019Quote: ... RNA integrity was evaluated by electrophoresis in denaturing agarose gel (1% agarose, 5% formamide, 1X TAE) stained with 3x Gel Red (Biotium Inc., CA, USA). The RNA was quantified using the Qubit RNA Assay Kit (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... according to manufacturer’s protocol after a couple of days and CRISPR/Cas9 induced editing efficiency was analyzed by PCR and separation of amplicon on 2% agarose gel containing 1:10.000 GelRed nucleic acid gel stain (41003, Biotium Inc., Fremont, CA, USA). Amplicons were purified by MinElute Gel extraction kit (28606 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were then washed once with NE solution and incubated in 5 µg/mL wheat germ agglutinin conjugated to Alexa Fluor 405 (Biotium cat#29028-1) diluted in NE solution for 2.5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High-molecular weight genomic DNA was confirmed from these extractions by visualizing each isolation on a 1% agarose gel stained with GelGreen® nucleic acid stain (Biotium, Hayward, CA, USA). Isolation quantity and quality was assessed using a Qubit™ fluorometer and a NanoDrop™ One (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Live stain cells were washed twice using 100 µl ASW and then incubated for 10 min in 50 µg/ml WGA-CF633 conjugate (Biotium, Cat No #29024-1) in ASW ...