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Citations for Biotium Inc. :
1 - 50 of 117 citations for PCR Master Mix since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and qPCR was performed using Eva Green PCR Master Mix (Biotium) from nine regenerating fragments in four (Figure 4.2 ...
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bioRxiv - Genetics 2021Quote: ... Quantitative PCR (qPCR) was preformed using the Forget-Me-Not qPCR Master Mix (Biotium) with a BioRad iCycler thermocycler ...
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bioRxiv - Immunology 2021Quote: ... the Fast EvaGreen qPCR Master Mix (Biotium), and a ABI7300 Real-Time PCR System ...
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bioRxiv - Bioengineering 2021Quote: ... Eva Green Master Mix (Biotium, Fremont, CA) was used with custom-made forward and reverse primers (Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... using Fast Plus EvaGreen qPCR master mix (Biotium). Serial dilutions of 106-101 copies per reaction of MCMV genome containing BAC (clone pSM3fr-MCK-2fl 3.3 [51] ...
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bioRxiv - Microbiology 2023Quote: ... using Fast Plus EvaGreen qPCR master mix (Biotium). Cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL Fast Evagreen qPCR Master Mix (Biotium, 31003), 2 μL of nuclease-free water ...
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bioRxiv - Synthetic Biology 2019Quote: ... The primers (Microsynth, Balgach) were used in the Real-Time PCR reaction with Fast EvaGreen® qPCR Master Mix (Biotium, USA) on ABI StepOnePlus (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... We used Fast Probe Master Mix (Biotium Inc. Fremont, CA) and followed the recommended preparation protocol (Standard Biotools 2015b) ...
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bioRxiv - Neuroscience 2022Quote: ... The resulting cDNA was diluted five-fold and RT-qPCR was performed with Eva™ Green PCR Master Mix (Biotium, Fremont, CA) in a StepOne Plus Real-Time PCR Platform (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed with the Fast EvaGreen qPCR Master Mix (Biotium) and measured on a ABI7300 Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... using Forget-Me-Not EvaGreen qPCR Master Mix with low ROX (Biotium). Data quantification was performed using the mak3i module of the qpcR software library (version 1.4-0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... qPCR assays were run with Forget-Me-Not Master Mix (Biotium 31044) in an Eppendorf Realplex 2 or BioRad CFX96 machine ...
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bioRxiv - Microbiology 2020Quote: ... 2X Forget-Me-Not Universal Probe Master Mix with ROX (Biotium 31044-1) was used to perform Q-RT-PCR in an Applied Biosystems StepOnePlus™ Real-Time PCR System Thermal Cycling Block ...
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bioRxiv - Neuroscience 2023Quote: ... Copy number qPCR was performed using Forget-Me-Not qPCR Master Mix (Biotium) on a QuantStudio5 system.
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bioRxiv - Microbiology 2020Quote: ... The 10 µL qPCR reactions contained 1 µL of sample and 9 µL of master mix (0.25 mM of each primer, 1X Forget-Me-Not EvaGreen qPCR mix (Biotium, Fremont, CA), and 0.4 mg mL-1 BSA ...
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bioRxiv - Microbiology 2022Quote: ... Each qPCR reaction contained 10μL of Master Mix EvaGreen 2X (Biotium, San Francisco, US), 1μM of each primer ...
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bioRxiv - Genomics 2020Quote: ... qPCR reactions were performed using the Forget-Me-Not™ qPCR Master Mix (Biotium) in 20 μL volumes containing 1 X Forget-Me-Not™ qPCR Master Mix ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed using Fast Plus EvaGreen® qPCR master mix High ROX (Biotium). Primers for genes of interest and genes of reference (GAPDH and HPRT1 ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium, USA). Data are presented as relative mRNA levels of the gene of interest normalized to GAPDH mRNA levels ...
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bioRxiv - Microbiology 2023Quote: ... and used in a qPCR using a ready-to-use mix containing a fluorescent-intercalating dye (EvaGreen® Fast qPCR Master Mix, Biotium, San Francisco, USA). After the amplification steps ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was carried out using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium; 31041-1) in the QuantStudio 7 Flex Real-Time PCR System (Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Microbiology 2020Quote: ... 1 μl of gDNA was used as template in a 20 μl qPCR reaction containing 10 μl of 2x Fast-Plus EvaGreen Master Mix with low ROX dye (Biotium, Inc. via VWR, Germany), 10 pmol of each forward and reverse primer (Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... antibody labeling kit Mix-n-Stain CF640R (Biotium); heme (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2020Quote: ... added to a qPCR mix (0.275 μL EvaGreen [31000, Biotium, Fremont ...
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bioRxiv - Systems Biology 2019Quote: ... Mix-n-Stain CF555 and CF640R antibody labeling kits were purchased from Biotium Inc ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were visualised with GelRed (Biotium, USA) gel electrophoresis and gel-purified ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were visualized with GelRed dye (Biotium) after agarose gel electrophoresis using a GelDoc imaging system (BioRad).
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bioRxiv - Cell Biology 2023Quote: ... conjugated to CF555 (Mix-n-Stain CF Antibody Labeling Kit, Biotium, Fremont, CA, USA)
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bioRxiv - Immunology 2020Quote: ... and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium, Fremont, CA, USA), respectively ...
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bioRxiv - Genomics 2022Quote: A PMAxx Real-Time PCR Bacterial Viability Test (Biotium Inc.), designed for selective detection of viable S ...
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bioRxiv - Cell Biology 2020Quote: ... PCNA antibody was pre-labeled using a Mix-n-StainCF-568 Dye Antibody Labeling Kit (Biotium; 92275) accordingly to the manufacturer protocol.
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bioRxiv - Cell Biology 2022Quote: ... primary antibodies were directly conjugated to fluorophores using Mix-n-Stain CF Dye Antibody Labeling Kits (Biotium) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Dye conjugation was performed using the Mix-n-Stain CF405M Antibody Labeling Kit (Biotium, Fremont, CA USA). Anti-TERT antibody conjugated with CF405M was diluted at 1:5 in storage buffer.
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bioRxiv - Cell Biology 2023Quote: ... primary antibodies were first labelled with fluorescent dyes using the Mix-n-Stain antibody labelling kit (Biotium) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using EvaGreen (Biotium, Catalog no: 89138-982) and the Biorad CFX Connect Real-time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were analyzed with 1.5% agarose gel containing GelRed (Biotium) in a 1X TAE buffer and subsequently captured the gel images using G:Box EF2 (Syngene ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were visualized by electrophoresis on agarose gel containing GelRed (Biotium) and band sizes were evaluated against a 100 bp molecular weight marker (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... The qPCR was performed using EvaGreen Real-time PCR (Biotium, Hayward, USA) according to the manufacturer’s recommendations using 1µl EvaGreen Real-time PCR (Biotium ...
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bioRxiv - Molecular Biology 2022Quote: PCR products were mixed with 1x GelRed prestain loading buffer (Biotium #41010), separated on a 2% or 4% agarose gel (Seakem LE agarose ...
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bioRxiv - Molecular Biology 2019Quote: ... APC-conjugated normal mouse IgG was produced using the Mix-n-Stain™ APC Antibody Labeling kit from Biotium Inc ...
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bioRxiv - Neuroscience 2023Quote: ... CAR antibodies were conjugated with the CF647 fluorophore (CAR-647) using the Mix-n-Stain antibody labeling kit according to the manufacturer’s protocol (Biotium). Sections were processed first with rabbit polyclonal Cav1.4 or EAAT2 antibodies and corresponding secondary antibodies as described above ...
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bioRxiv - Immunology 2021Quote: ... PCR products were run on 1% agarose gel and visualised using GelRed (Biotium) and transgene sequence confirmed by Sanger sequencing at the Australian Genome Research Foundation (AGRF).
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bioRxiv - Molecular Biology 2020Quote: ... PCR products were run on a 1.2% agarose gel containing GelRed™ (Biotium). After electrophoresis only a single band was observed ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was run on a ViiA 7 with SYBR green mastermix (Biotium). 18S was used as the human cell housekeeping gene and Ywhaz was used as the mouse housekeeping gene ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were analyzed on a 1 % agarose gel containing GelRed (0.01%, Biotium) and visualized under UV light.
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bioRxiv - Genetics 2023Quote: ... The PCR products were resolved on a 1.5% agarose gel containing GelRed (Biotium) and visualized under UV light to distinguish expanded from non-expanded CAG repeats ...
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bioRxiv - Genetics 2019Quote: ... The number of amplification PCR cycles for each sample was determined individually by performing a qPRC reaction of 7.5 µl aliquote of the mix with an addition of the EvaGreen dye (Biotium, Fremont, U.S.). The amplified libraries were SPRI purified (upper cut 0.5x ...
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bioRxiv - Developmental Biology 2020Quote: ... was conjugated to Alexa 647 using the Biotium Mix-n-Stain R-PE(PE) Antibody Labeling kit (Biotium, cat no: 92298) following the manufacturers protocol ...