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Citations for Biotium Inc. :
51 - 100 of 102 citations for Necdin NDN Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and stained with Annexin V-APC antibody and DAPI (0.1 μg/ml, Biotium, BT-40043) using the Annexin V Apoptosis Detection Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... we utilized the anti-CD44 mouse monoclonal antibody (CF488A) [clone: HCAM/918] (Biotium, BNC880918-100) with affinipure Fab fragments goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with secondary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 1 hour at RT ...
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bioRxiv - Cell Biology 2019Quote: ... CF680- and CF568-labeled secondary antibodies were prepared through reactions of the corresponding succinimidyl esters (Biotium) with secondary antibodies from Jackson ImmunoResearch.
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bioRxiv - Cell Biology 2021Quote: ... The following secondary antibodies were used: Donkey CF-488A anti-rat IgG (Biotium 20027, 1:000) and CF-568 anti-rabbit IgG (Biotium 20698 ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies used were CF680 goat anti-mouse IgG (Biotium, cat no: 20065; dilution 1:15000) and CF770 goat anti-rabbit IgG (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed in PBS and incubated with the following secondary antibodies (1:500, Biotium) for 2 h at room temperature ...
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bioRxiv - Genomics 2020Quote: Antibodies were detected using either the tyramide signal amplification (TSA) (33002 CFF488A Streptavidin HRP, Biotium, Fremont, CA) and streptavidin-conjugated Alexa-fluor 488 (1 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were Donkey anti-Goat IgG conjugated with CF488 (for GFP, 1:1000, #20016-1, Biotium), Donkey anti-Rabbit IgG conjugated with CF594 (for pERK ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated with secondary antibodies donkey anti-rat 568 (BIOTIUM, Cat. No. 20092, 1:1000), donkey anti-rabbit 647 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Dye conjugation was performed using the Mix-n-Stain CF405M Antibody Labeling Kit (Biotium, Fremont, CA USA). Anti-TERT antibody conjugated with CF405M was diluted at 1:5 in storage buffer.
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bioRxiv - Neuroscience 2020Quote: Retinal sections immunolabeled with α-Rb MCT2 primary antibody were incubated with TrueBlack Lipofuscin Autofluorescence Quencher (Biotium) for thirty minutes after blocking and before primary antibody incubation ...
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bioRxiv - Bioengineering 2022Quote: ... was fluorescently conjugated in-house with an amine-reactive fluorescent dye antibody conjugation kit (Biotium, Fremont, CA) and used as a detection label.
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bioRxiv - Cancer Biology 2023Quote: ... tissues were incubated with secondary antibodies coupled with fluorescent dye (dilution 1/800, Biotium, Fremont, CA, USA) according to the species in which the primary antibodies were produced for 3h at RT and with or without rhodamine-phalloidin (dilution 1/200 ...
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bioRxiv - Biochemistry 2021Quote: ... Goat Anti-Rabbit (Cat. #89138-520) and anti-mouse CF680 (Cat. # 20067) secondary antibody were purchased from Biotium. Goat anti-rabbit DyLight 800 secondary antibody was purchased from Thermo Fischer Scientific (Cat ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... organs were incubated as above with Alexa Fluor 488 secondary antibody and 0.4 U/ml CF568 phalloidin (Cat#00044, Biotium). Tissue was washed three times in PBS and mounted on a glass slide in ∼50 µl of Vectashield and covered with a #1.5 thickness 22 x 22 mm cover glass (Cat#2850-22 ...
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bioRxiv - Molecular Biology 2019Quote: ... APC-conjugated normal mouse IgG was produced using the Mix-n-Stain™ APC Antibody Labeling kit from Biotium Inc ...
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bioRxiv - Neuroscience 2022Quote: ... Brain sections were washed and incubated in secondary antibody solution with Goat anti-Rabbit Alexa 568 (Biotium, 1:500) and Goat anti-Mouse IgG1α Alexa 488 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... CAR antibodies were conjugated with the CF647 fluorophore (CAR-647) using the Mix-n-Stain antibody labeling kit according to the manufacturer’s protocol (Biotium). Sections were processed first with rabbit polyclonal Cav1.4 or EAAT2 antibodies and corresponding secondary antibodies as described above ...
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bioRxiv - Developmental Biology 2020Quote: ... The resulting signals were detected by incubation with secondary antibodies conjugated to fluorophores (Thermo Fisher Scientific, Biotium, or Jackson ImmunoResearch). Sections were counterstained with DAPI ...
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bioRxiv - Bioengineering 2020Quote: ... was used to dye the actin cytoskeleton alongside with CF488 anti-rabbit IgG antibody (Biotium, Fremont, USA, 1:500 dilution) in 0.5% w/v BSA 0.1% v/v Triton X-100 in PBS for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... samples were labeled with an anti-Myosin 7A antibody (#25-6790 Proteus Biosciences, 1:400) and goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG, 1:1000, Biotium; CF594-conjugated donkey anti-guinea pig IgG ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... FGOs were incubated with anti-rabbit IgG (H+L) CF 488A and anti-mouse IgG (H+L) CF 594 secondary antibodies (Biotium) (dilution 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Developmental Biology 2020Quote: ... was conjugated to Alexa 647 using the Biotium Mix-n-Stain R-PE(PE) Antibody Labeling kit (Biotium, cat no: 92298) following the manufacturers protocol ...
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bioRxiv - Microbiology 2019Quote: ... with CF™ 568 dye (Mix-n-Stain™ CF® Dye Antibody Labeling Kits - CF®568, Biotium Inc, CA), and followed the nonpermeable approach for IFA [12] to investigate the interaction between Ab and ookinetes ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for Porin ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for NOP1 ...
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bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times to remove secondary antibody and were then stained with 4’,6-diamindino-2-phenylindole (DAPI; Biotium, 40011) diluted in PBS for 20 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-CLEC4F was from R&D (#MAB2784) and coupled to CF568 using the Mix-n-Stain CF568 Antibody Labeling Kit (Biotium, #BTM92235). All antibodies were used at 1:100 dilution ...
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bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then incubated with primary antibodies in 2% BSA on a gentle shaker at room temperature for 3 days followed by three times of 1X PBS washing and incubation of secondary antibodies and CF® Phalloidin (Biotium) staining for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Cell Biology 2023Quote: ... antibodies were used at 1μg/mL overnight at 4°C and were subsequently detected with STORM-appropriate secondary antibodies (Biotium; 20836 and 20811). STORM acquisition was facilitated with an N-STORM system (Nikon Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were washed with PBS three times for 20 minutes and then incubated with commercial dye-conjugated secondary antibodies diluted in blocking solution (AF647-conjugates from Jackson ImmunoResearch Laboratories, West Grove, PA, and CF568-conjugates from Biotium, Fremont, CA) for 1 hour at room temperature ...