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Citations for Biotium Inc. :
301 - 345 of 345 citations for Mouse T cell receptor alpha chain C region TCRA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were pre-treated 16-20 h with CellBrite®650 according to the manufacturers’ instructions (Biotium). For the analysis of ALIX-vesicle motility ...
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bioRxiv - Neuroscience 2023Quote: ... cells were pre-incubated with Fura-2·AM or Fluo-4·AM (30 minutes, 1 μM, Biotium) at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Then, cells were resuspended in PI Staining Solution (3.8mM sodium citrate, 50 μg/ml PI (Biotium, USA), 40 μg/ml RNase A (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... Imaging of microtubules was performed by staining the microtubules with ViaFluor Live Cell Microtubule Stain 647 (Biotium) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were blocked for 1 hour at room temperature with 1x Fish Gelatin Blocking Agent (Biotium, #22010) in wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed twice with 1X PBS and then incubated with TrueBlack IF Background Suppressor (Biotium) for 30 minutes at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then incubated with 25 µM Alexa Fluor 568-hydroxylamine (Fluoroprobes) or CF680R-Aminooxy (Biotium) in the presence of 10 mM aniline (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Mix-n-Stain CF Dye Antibody Labeling Kits (CF-680, #92282, and CF-770, #92285) were purchased from Biotium (San Francisco, CA, USA).
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies CD23 and IgM were conjugated with Mix-n-Stain fluorescent protein tandem dyes antibody labeling kit for APC-CF750T (Biotium, Fremont, CA, USA) and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium ...
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bioRxiv - Immunology 2019Quote: ... Apoptosis assays were performed using Nucleocounter NC-3000 and a Flexicyte apoptosis/necrosis detection kit based on staining with caspase 3 substrate NucView 488 and RedDot 2 (all Biotium, Cambridge Bioscience, UK). Apoptotic cells are defined as NucView488 positive and RedDot2 negative ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with 1 µg/mL of wheat germ agglutinin (WGA) conjugated to CF568 (Biotium; #29077-1) and 1 µg/mL anti-CD154 (CD40L ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were washed twice with PBS and incubated with PBS containing 50 μg/mL propidium iodide (Biotium, 40017) and 100 μg/mL RNase A (Omega ...
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bioRxiv - Neuroscience 2022Quote: Lysosomal activity was measured for the BV2 cell line and iMGLs using the LysoViewTM 540 reagent (Biotium, 70061) and Incucyte S3 Live-Cell imaging ...
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bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with PBS three times and coverslips were mounted onto glass slides in EverBrite Mounting Medium (Biotium). Cells were imaged using an EVOS FL microscope (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... the growth medium was removed and cells were incubated with 1 μM CellBrite™ NIR680 (Biotium, Fremont, CA, USA) for 20 min ...
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bioRxiv - Immunology 2023Quote: ... Dead cells and doublets were excluded from analyses based on staining of 7-amino-actinomycin D (7-AAD) (Biotium) or Zombie yellow (catalog ...
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bioRxiv - Bioengineering 2023Quote: ... The cell viability was documented via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2021Quote: Spermine fluorescent labeling with CF405M or CF594 dyes was performed using a Mix-n-Stain Small Ligand Labeling Kit (Biotium, Cat# 92362 and 92352, respectively) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometery was used to assess mitochondrial number and membrane potential of the cells treated with Mito Tracker (Biotium, 70075) and TMRE (tetramethylrhodamine ethyl ester ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: ... then maintained in complete darkness for two days prior to incubation with a cell viability stain (Live-or-Dye 488/515 (Biotium). Briefly ...
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bioRxiv - Genetics 2020Quote: To test the relative expression of XIST across cell lines quantitative real-time PCR (qPCR) was carried out using EVAgreen (Biotium) and Maxima Hot Start Taq (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged to pellet (table-top centrifuge, 3000 xg, 15 min) and resuspended in PBS containing MitoView 633 (Biotium). MitoView 633 accumulates in the inner membrane as a function of membrane potential ...
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bioRxiv - Microbiology 2024Quote: ... The washed cells were stained with 0.1 mg/ml CF®488A wheat germ agglutinin conjugate (Biotium, Fremont, CA, USA) in PBS for 5 min at room temperature and washed two more times with PBS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then washed with PBS buffer (pH 7.4) and resuspended in complete RPMI containing NucSpot® Live 488 (Biotium, #40081) following the manufacturer recommendations ...
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bioRxiv - Microbiology 2022Quote: ... immediately spread as a single-cell layer between a quartz slide and coverslip and sealed with a CoverGrip sealant (Biotium, USA). Two-dimensional Raman maps were obtained with a laser excitation at 532 nm (20 mW power at the focal plane ...
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bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were mounted using EverBrite mounting medium with 4′,6-diamidino-2-phenylindole (Cat No. 23002, Biotium, Fremont, CA, USA). Images were recorded by Olympus IX51 microscope with ToupTek camera and with the same exposure parameters (Alexa Fluor™ 555 ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the cell pellets using the hot-phenol procedure and followed by biotinylation with MTSEA-Biotin-XX (Biotium, USA, Fremont) as previously described (27 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF®488A (Biotium®) for 30 minutes at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 1×106 cells from each liver were stained for various cell markers: Live-or-Dye™ fixable viability dye in APC-Cy™7 (Biotium, Cat ...
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bioRxiv - Microbiology 2024Quote: ... The bladder cells were stained for membrane (CellBrite Steady Membrane 405) and nucleus (NucSpot Live Cell 650) for 30 minutes in pre-warmed EpiLife as per manufacturer’s instructions (Biotium, San Francisco, USA). After the staining step and two washes with 1 x PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Aliquots of detached cells (approximately 2×106) were incubated with a series of CF®640R succinimidyl ester-labeled (Biotium, USA, cat. 92108) ACE2 extracellular domain (AA Q18 – S740 ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... by IP3R-mediated ER Ca2+ release were measured in cells loaded with membrane-permeant AM ester form of Fluo-3 (Fluo-3-AM) (Biotium, Fremont, CA, USA) and Fura-Red ...
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bioRxiv - Microbiology 2023Quote: ... and DNA from membrane-compromised bacterial cells was removed by treatment of coral homogenates and tissue airbrushed blanks with the viability dye PMAxxTM (Biotium, Fremont, CA, USA). PMAxx is a DNA-intercalating agent that forms photo-induced crosslinks making the bound DNA inaccessible for downstream molecular applications ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability was evaluated in the control condition (cells grown in the complete medium) and in the test conditions (cells grown in the hyperosmolar media) via a resazurin-based assay (Biotium, Inc., Fremont, CA, USA) performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dried and slides were coverslipped using fluorescent mounting medium (VectaShield, Vector Laboratories, Inc., Burlingame, CA, USA; or EverBrite, Biotium, Inc, Fremont, CA USA). In some studies ...