Labshake search
Citations for Biotium Inc. :
51 - 100 of 334 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 5 μL of 5 μg/mL Propidium Iodide (Biotium) for cell death detection ...
-
bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were washed twice and loaded with 25 nM TMRM and either 5 μM Nucview 488 (Biotium) or 5 μM Fluo4-AM (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2021Quote: ... In neurons Pink Flamindo was co-localized with Mito-View 405 (Biotium, Cat #70070-T). Live cells confocal imaging was performed on confocal microscope described in methods section 6.
-
bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1 h at 37°C in mKRB supplemented with native coelenterazine (5 µM, BIOTIUM), and then incubated for 15 minutes in mKRB supplemented with either TPEN (500 µM ...
-
bioRxiv - Bioengineering 2023Quote: ... the Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Biotium, UK) was used according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... Bacteria were diluted in 1mL of PBS and stained with CellBrite Fix 488 (Biotium, 30090-T) or CellBrite Fix 555 (Biotium ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS-T and the lipofuscin autofluorescence was quenched using Trueblack (Biotium, 23007) for 2 min at RT and DAPI was added to stain the nucleus ...
-
bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
-
bioRxiv - Cell Biology 2022Quote: ... 5 µM CF647 azide (Biotium), 200 µM CuSO4 ...
-
bioRxiv - Immunology 2023Quote: ... 5 μL of MTT (Biotium) was dispensed in each well and further incubated at 37°C for 1h ...
-
bioRxiv - Genomics 2022Quote: ... Cell-free DNA was quantified using the AccuBlue NextGen DNA Quantification Kit (Biotium).
-
bioRxiv - Neuroscience 2020Quote: ... on a 1% Agarose Gel (LifeTech 16500500; 1X TBE buffer, LifeTech 16500500) with GelRed (Biotium 41003-T) for gel electrophoresis ...
-
bioRxiv - Cell Biology 2022Quote: ... and stained with the viability markers DAPI (1 μg/ml) and RedDOT (1:500, #40060-T, Biotium) before being sorted on a FACSAria II (BD Biosciences).
-
bioRxiv - Neuroscience 2022Quote: ... Human transferrin-CF488A conjugate was purchased from Biotium (cat. #00081). Mouse monoclonal anti-GFAP (cat ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-mouse and anti-human IgG coupled to CF488R (Biotium) or CF640R (Biotium ...
-
bioRxiv - Genomics 2022Quote: ... 5% (v/v) EvaGreen (31000, Biotium) was added to the amplification mixture to monitor the progress of library amplification ...
-
bioRxiv - Neuroscience 2022Quote: ... or 10 μM fluorescently labeled human transferrin (Tf488; Biotium, cat. #00081) for 4 h at 37 °C under gentle agitation ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were analyzed using the Steady-Luc™ Firefly HTS Assay Kit (Biotium, Freemont, CA). Luminescence was measured using a Synergy2 plate reader (Biotek Inc ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were lysed using lysis buffer supplied with Renilla Luciferase Assay Kit 2.0 (Biotium). 20 µl of cell lysate was mixed with 100 µl of freshly prepared working solution and the luminescence was recorded using a TECAN spectrophotometer.
-
bioRxiv - Cancer Biology 2020Quote: ... Ethidium Homodimer I (Biotium; 61926-22-5) per manufacturer protocol and expression of phenotypical markers was measured at day 5 via immunostaining ...
-
bioRxiv - Physiology 2021Quote: ... with or without 5 µM dipicrylamine (Biotium), using a homebuilt multi-barrel flow pipe (see above) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product by mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
-
bioRxiv - Biochemistry 2023Quote: ... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
-
bioRxiv - Bioengineering 2023Quote: The percent toxicity was calculated using an XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) colorimetric assay (Biotium) wherein viable cells form an orange formazan product upon reduction ...
-
Proteasomal degradation of human SERINC4: a potent host anti-HIV-1 factor that is antagonized by NefbioRxiv - Microbiology 2020Quote: ... cells were lysed and intracellular luciferase activities were determined using Firefly Luciferase Assay Kit 2.0 (Biotium). These luciferase activities were used to calculate viral infectivity.
-
bioRxiv - Molecular Biology 2020Quote: ... Autofluorescence quenching: Human sections were treated with TrueBlack Lipofuscin Autofluorescence Quencher (TLAQ) (Biotium) per manufactures instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
-
bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... 5 u ml−1 phalloidin CF555 (Biotium, 00040) were added together with the secondary antibodies ...
-
bioRxiv - Molecular Biology 2023Quote: TUNEL assay was used to detect cell apoptosis using the CF605R TUNEL Assay Apoptosis Detection Kit (Biotium) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked for 1 hr at room temperature with 5% non-fat dry milk (Apex #20-241) or 5% BSA (Biotium #22013) in TBST (10 mM Tris ...
-
bioRxiv - Bioengineering 2024Quote: Cell proliferation was evaluated using the XTT (2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay (Biotium Inc., Fremont, CA) according to the manufacturer’s instructions ...
-
Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... cells were rinsed and 1:1000 CellBrite Steady 650 membrane dye and 1:1000 kit enhancer (Biotium #30108) was added in Fluorobrite DMEM +10% FBS for 30 minutes at 37℃.
-
bioRxiv - Cell Biology 2023Quote: ... and DAPI (5 μg/ml) (Biotium, Cat #BT-40043). Staining with DAPI was done for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 μg/ml of DAPI (Biotium; Cat #BT-40043) was used as a viability dye ...
-
bioRxiv - Immunology 2023Quote: ... and NucView-488 at 5 μM (Biotium, Cat. 10402). NucView-488 was selected to visualize activation of Caspase-3 in apoptosis ...
-
bioRxiv - Neuroscience 2024Quote: ... cocultures exposed to TSZ after 4.5 h after were stained using a propidium iodide-based (PI) cell viability kit (Biotium) according to manufacturer protocols ...
-
bioRxiv - Cell Biology 2020Quote: ... and APCs seeded on plastic (2D control) were assessed using fluorescent calcein AM/ethidium homodimer III (EtDHIII) Live/Dead assay (30002-T, Biotium Inc, Insight Biotech, UK) and Click-iT® EdU (5-ethynyl-2’deoxyuridine ...
-
bioRxiv - Neuroscience 2020Quote: ... applying 5 μl of Fluorogold (Hydroxystilbamine FluoroGold, Biotium, Fremont, CA) to the area ...
-
bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
-
bioRxiv - Neuroscience 2022Quote: ... and 48 h later luciferase activity was measured in cell lysates using the Firefly & Renilla Single Tube Luciferase Assay Kit (Biotium), according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... the IVT reaction was doped with 5-amino-allyl UTP (Biotium) at 1:10 (aminoallyl-UTP ...
-
bioRxiv - Developmental Biology 2023Quote: ... explants were incubated with fluorescently conjugated human transferrin-594 (CF 594; 25 μg/mL; Biotium Fremont CA) for 40 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
-
bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
-
bioRxiv - Molecular Biology 2019Quote: Viability of monolayers post-antibiotic treatment was assessed using the Viability/Cytotoxicity Assay for Animal Live & Dead Cells kit (Biotium, Fremont, CA) according to package insert ...