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Citations for Biotium Inc. :
1 - 50 of 391 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and the integrity was assessed by agarose gel electrophoresis (1% wt/vol agarose in Tris-acetate-EDTA) amended with 1:20,000 (v/v) nucleic acid stain GelRed (Biotium). The isolates were confirmed as A ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Chaperones and WT cell extracts were labeled with APC (Biotium # 92108) while Ugi was labeled with FITC (Biotium # 92103 ...
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bioRxiv - Biophysics 2020Quote: ... Human Transferrin – CF488A (Biotium) at 130 nM ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Human transferrin-CF488A conjugate was purchased from Biotium (cat. #00081). Mouse monoclonal anti-GFAP (cat ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse and anti-human IgG coupled to CF488R (Biotium) or CF640R (Biotium ...
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bioRxiv - Biophysics 2022Quote: ... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... or 10 μM fluorescently labeled human transferrin (Tf488; Biotium, cat. #00081) for 4 h at 37 °C under gentle agitation ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Molecular Biology 2020Quote: ... Autofluorescence quenching: Human sections were treated with TrueBlack Lipofuscin Autofluorescence Quencher (TLAQ) (Biotium) per manufactures instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Developmental Biology 2023Quote: ... explants were incubated with fluorescently conjugated human transferrin-594 (CF 594; 25 μg/mL; Biotium Fremont CA) for 40 minutes ...
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bioRxiv - Immunology 2021Quote: ... stained with one-step blue protein gel stain (Biotium, ref. 21003), and by SEC-HPLC with an xbridge protein BEH SEC (Waters ...
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bioRxiv - Cell Biology 2021Quote: ... Protein gels were transferred onto nitrocellulose membranes and subsequently stained with Ponceau S (Biotium) before blocking in 5% BSA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Thermal melt measurements were using a GloMelt Thermal Shift protein stability kit from Biotium on a Qiagen Rotor-gene Q qPCR instrument ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... protein expression of the FRHΔ100 plasmid was induced with 0.2 mM IPTG (Biotium, 10021), at 18°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Genetics 2023Quote: ... Total protein stain was then conducted by diluting CF568-labeled succinimidyl ester (Biotium, 92131) at 1:100,000 in water containing 0.1 M NaHCO3 and 25 mM Na2CO3 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.9) and subjected to SDSCPAGE followed by Lumitein Protein Gel Stain (Biotium, Hayward, CA, USA) and immunoblotting ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Wheat Germ Agglutinin (WGA) (1:1000, 29022-1, Biotium), and Non-muscle Myosin II A (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... goat-anti-mouse CF680 (1:500 dilution, Biotium, 20065-1) and goat-anti-mouse Cy3 (1:500 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... we utilized fluorophore-conjugated forms of the lectin wheat germ agglutinin (WGA) (Biotium, 29022- 1, 29062-1, 29025-1) to outline all cellular membranes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Neuroscience 2023Quote: ... along with 1% BSA and mixed 1:200 with RedDot (40060, Biotium). The samples were then isolated from debris by fluorescence -activated nuclei sorting (FANS) ...
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bioRxiv - Genomics 2021Quote: ... 1 × EvaGreen dye (Biotium) was added to the amplification mixture to monitor the PCR amplification ...
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bioRxiv - Cell Biology 2024Quote: ... + 1:1000 Enhancer (Biotium) and incubated for ∼10-15min before starting imaging ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 μL of aniline (diluted 1:10 in water) and 1 μL of 100 mM biocytin hydrazide (Biotium, 90060). The reaction proceeded while rotating at 4°C for 90 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Cell Biology 2022Quote: ... followed by incubation with RedDot™1 Far-Red Nuclear Stain (1:200, Biotium) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... goat Anti-Guinea Pig IgG Polyclonal CF-488A (1:500, Biotium, cat#: 20017-1), cy5-AffiniPure Donkey Anti-Guinea Pig IgG H&L (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Phalloidin CF405 (1:200; Biotium) or MitoSpy™ NIR DiIC1 dye (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... Hoecsht 33342 (1:500; Biotium), and rhodamine-phalloidin (1:20 ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (1:2000; Biotium 40011), Hoechst (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 x EvaGreen dye (Biotium), 3 µl DNA template dilution (1×104 copies/reaction ...
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bioRxiv - Genetics 2022Quote: ... DAPI (Biotium#40043, 1:500) was used as a nuclear counterstain ...
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bioRxiv - Cell Biology 2021Quote: ... Phalloidin CF647 1:100 (Biotium) in PBS + 1% FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (1:1000, Biotium). Epidermal cells for RNA-sequencing experiments were membrane GFP+ ...
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bioRxiv - Cell Biology 2022Quote: ... and DAPI (1:1000, Biotium). We used Flow cytometry was performed on a BD LSRII cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2022Quote: ... and 1× EvaGreen (Biotium, 31000). 5 ng of the purified PCR1 of each sample was used as input for the final PCR2 ...
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bioRxiv - Genomics 2023Quote: ... and 1× EVA green (Biotium). The qPCR (Bio-Rad CFX Real-Time system ...
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bioRxiv - Developmental Biology 2023Quote: ... and WGA488 (1 μg/ml; Wheat Germ Agglutinin CF®488A Conjugate; Biotium, #BT29022-1) membrane staining was added to single wells immediately before imaging on an Opera Phenix™ High-Content Screening System (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: ... a PDMS well was filled with a 1:1000 dilution of YO-PRO-1 (Biotium), which was then incubated for an hour ...