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Citations for Biotium Inc. :
451 - 500 of 538 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:400) followed by application of goat anti-rabbit CF568 (Biotium) secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a 1:500 dilution and detected with HRP-conjugated goat anti-mouse secondary antibody (Biotium #20400-1mL) at 1:500 together with Opal 520 reagent at 1:1,000 (Akoya Biosciences #OP-001001).
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bioRxiv - Bioengineering 2023Quote: ... CD81 and IgG control antibodies (Supplementary Table 1) were depleted from sodium azide using a 10kDa MWCO filter ultrafiltration vial (Biotium). Ten microgram of each antibody was conjugated overnight at 4°C with 40µg/mL NHS-AlexaFluor647 (AF647 ...
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bioRxiv - Biophysics 2023Quote: ... Triton X-100 in PBS with a three-minute incubation period during each wash before being labeled with donkey anti-rabbit secondary antibodies conjugated with dye CF568 (20098-1, Biotium) at a 1:100 dilution in 1% (w/v ...
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bioRxiv - Genomics 2023Quote: ... 5 μg of antibody in PBS (BSA-free) is mixed with 1 μl 0.33 mM CF750 Dye SE/TFP esters (Biotium 92142), Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific A20006) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2 minutes in TrueBlack Lipofuscin Autofluorescence Quencher (Biotium cat no. 23007) with 3 subsequent 1x PBS rinses before mounting and imaging.
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bioRxiv - Developmental Biology 2019Quote: ... DNA band was visualized on 2% agar gels stained with GelRed (41003, Biotium). All mammary gland tissues used in this paper are from female mice.
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bioRxiv - Neuroscience 2020Quote: ... The sections were then left to cool down at room temperature and subsequently incubated for 1 minute in TrueBlack lipofuscin autofluorescence quencher (Biotium, USA) diluted 1:20 in 70% ethanol ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h and stained with a JC-1 Mitochondrial Membrane Potential Detection Kit using the manufacturers protocol (Biotium, Fremont, CA). The dye forms JC aggregates (orange ...
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bioRxiv - Developmental Biology 2021Quote: ... centrifuged for 10 min at ∼13,000 x g and then 25uL of the clear supernatant lysate was used separately in firefly (Biotium #30085–1) and renilla (Biotium 300821 ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were separated by electrophoresis on 1% agarose gel and visualized by staining with GelRed™ Nucleic Acid Gel Stain (Biotium). Nested PCR products were pooled and purified using Monofas® DNA purification Kit I (GL Sciences) ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for Porin ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-rabbit secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-520; 1:10000 dilution) was used to probe for PGK1 and GFP ...
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bioRxiv - Cell Biology 2019Quote: ... A goat α-mouse secondary antibody conjugated to a 680 nm emitting fluorophore (VWR/Biotium; Cat # 89138-516; 1:10000 dilution) was used to probe for NOP1 ...
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bioRxiv - Neuroscience 2020Quote: ... The cover-slips were rinsed with PBSNG (3×20 min) and incubated with highly cross-absorbed donkey secondary antibodies conjugated to CF™488/594/647-Dye (1:400; Biotium, #20014 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cells were washed three times in antibody dilution buffer and then incubated in the dark for 30 min with 1:1000 diluted secondary antibody (Goat anti-mouse AF488, Biotium, #12C0403). Cells were again washed three times before being resuspended in sort buffer (1x PBS ...
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bioRxiv - Plant Biology 2023Quote: ... Amplicons products were analyzed by electrophoresis on a 1% agarose gel in Tris-acetate-EDTA (TAE) buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Genomics 2023Quote: ... PCR products were analyzed by electrophoresis on a 1% agarose gel in TAE buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Neuroscience 2023Quote: ... at room temperature for 60 min before incubation in PBS-T 0.3% with 3% NDS and CF405-conjugated streptavidin (1:1000; Biotium, CA, USA). After 90 min slices were washed in PBS (4x 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... centrifuged for 10 min at ∼13,000×g and then 50 µl of the clear supernatant lysate was used separately in firefly (Biotium #30085-1) and renilla (Biotium 300821 ...
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bioRxiv - Systems Biology 2023Quote: ... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... The resultant triplicate PCR products for each sample were pooled and quantified using a 1% agarose gel stained with GelRed (Biotium, CA, USA). Quantified pooled triplicates were then combined in equimolar concentrations based on gel quantification to create an amplicon library ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Equal volumes of PCR reactions were run on a 1% agarose gel and visualized with GelRed Nucleic Acid Stain (Biotium, Fremont, CA).
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were washed three times with DPBS, and the solution of To-pro3 (Thermo Fischer Scientific, T3605, 1:1000) or Hoechst (Biotium, 33342, 5μM) was added and incubated for 30 min at RT in the dark to stain the nuclei ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
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bioRxiv - Bioengineering 2021Quote: ... the products were analyzed using electrophoresis on a 2% agarose gel containing GelRed (Biotium) and visualized under UV light.
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bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by electrophoresis using a 2% agarose gel containing GelRed (Biotium) and visualized using an Azure c400 imager (Azure Biosystems) ...
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bioRxiv - Zoology 2022Quote: All PCR products were verified on a 2% agarose gel stained with GelRed (Biotium). From each of the 12 libraries (2*3 replicates for MiFish-U and 2*3 replicates for Mamm01 ...
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bioRxiv - Microbiology 2019Quote: ... Gel electrophoreses of PCR products were done using 1% Agarose gel matrix with the addition of 10,000X GelRed™ Nucleic Acid Gel Stain (Biotium, Inc. CA, USA). Samples were run alongside 100bp exACTGene™ DNA ladder (Fisher BioReagents™ ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
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bioRxiv - Evolutionary Biology 2020Quote: ... After electrophoresis in a 1.5% agarose gel stained with 2% GelRed (Biotium, Hayward, CA, USA), amplicons of the expected size were sequenced on both strands by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).