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Citations for Biotium Inc. :
451 - 500 of 533 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium, 23013) with 0.2% Tween-20 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were fixed with 4% PFA and stained with CF532 Wheat Germ Agglutinin (WGA) Conjugates (Biotium) and DAPI and imaged under a confocal microscope (Leica SP8) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were exposed to 10 μM SynaptoRed™ C2 (Equivalent to FM®4-64; Biotium; 70027) in Stimulation Buffer consisting of 31.5 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: Conduction velocity (CV) was quantified by optical voltage mapping using di-4 ANEPPS (15 μM, Biotium #61010) as previously described.28 Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The resultant triplicate PCR products for each sample were pooled and quantified using a 1% agarose gel stained with GelRed (Biotium, CA, USA). Quantified pooled triplicates were then combined in equimolar concentrations based on gel quantification to create an amplicon library ...
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bioRxiv - Cell Biology 2022Quote: ... tendons were washed six times in PBS and placed in secondary antibody solution containing fluorescent CF488 anti-mouse secondary antibody (1:200; Biotium, Fremont, CA), Hoecsht 33342 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... washed for 10 min with 0.5% PBS-T for three times and subsequently treated with Alexa Fluor 568- or Alexa Fluor 488-conjugated fluorescent secondary antibody (1:500; Biotium, 20103, 20012) 1 hr at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Equal volumes of PCR reactions were run on a 1% agarose gel and visualized with GelRed Nucleic Acid Stain (Biotium, Fremont, CA).
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bioRxiv - Pathology 2020Quote: ... PCSK9 promoter-driven firefly luciferase activity and control renilla luciferase activity were measured using the Firefly & Renilla Luciferase Single Tube Assay Kit (#30081-1; Biotium, Fremont, CA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were washed three times with DPBS, and the solution of To-pro3 (Thermo Fischer Scientific, T3605, 1:1000) or Hoechst (Biotium, 33342, 5μM) was added and incubated for 30 min at RT in the dark to stain the nuclei ...
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bioRxiv - Microbiology 2023Quote: ... 1 mL of each fecal inoculum was mixed with 2.5 µL of 20 mM propidium monoazide (PMA) dye (Biotium, Fremont, CA, USA). The samples were then incubated in the dark for 5 min ...
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bioRxiv - Cancer Biology 2023Quote: ... then washed with PBS five times and incubated overnight with Alexa Fluor 568 - conjugated anti-rabbit secondary antibodies (1:500, Biotium cat#20098) and DAPI dye ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... GloMelt (λEx = 468, λEm = 507 nm) and carboxyrhodamine (ROX; λEx = 588, λEm = 608 nm) dyes (CAT #33022-1) were purchased from Biotium (Fremont, CA). Bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were replaced with medium containing 5 µM NucView 488 caspase 3 substrate (Biotium Inc., #10403). After 30 min incubation at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing was performed using PCR-mixture-2 blue (AmpliSens, Russia) and EvaGreen (Biotium, USA) as a dye ...
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bioRxiv - Bioengineering 2021Quote: ... the products were analyzed using electrophoresis on a 2% agarose gel containing GelRed (Biotium) and visualized under UV light.
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bioRxiv - Microbiology 2021Quote: PCR products were checked in 2% agarose gels stained with GelRed™ (Biotium, USA) under UV light ...
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bioRxiv - Microbiology 2022Quote: ... PCR reactions were analyzed by electrophoresis using a 2% agarose gel containing GelRed (Biotium) and visualized using an Azure c400 imager (Azure Biosystems) ...
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bioRxiv - Zoology 2022Quote: All PCR products were verified on a 2% agarose gel stained with GelRed (Biotium). From each of the 12 libraries (2*3 replicates for MiFish-U and 2*3 replicates for Mamm01 ...
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bioRxiv - Microbiology 2019Quote: ... Gel electrophoreses of PCR products were done using 1% Agarose gel matrix with the addition of 10,000X GelRed™ Nucleic Acid Gel Stain (Biotium, Inc. CA, USA). Samples were run alongside 100bp exACTGene™ DNA ladder (Fisher BioReagents™ ...
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bioRxiv - Bioengineering 2022Quote: ... the cell aggregates were processed and imaged as described in the whole-mount immunofluorescence methods using Streptavidin (29072, Biotium, 1:500, CA, USA) and a DAPI (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were CF680- or CF770-conjugated anti-mouse and anti-rabbit IgG antibodies (1:5000, #20067 and #20077, Biotium, Hayward, CA, USA). Antibodies were diluted in Blocking One (Nacalai Tesque) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed twice and loaded with 25 nM TMRM and either 5 μM Nucview 488 (Biotium) or 5 μM Fluo4-AM (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... they were incubated with wheat germ agglutinin (WGA) conjugated to CF©488A (5 μg/ml Biotium) at room temperature for two hours ...
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Biochemistry 2023Quote: ... DNA binding experiments using plasmid-length substrates were performed under the same conditions except with 100 ng DNA per reaction and were run on 0.8% agarose gel at 4 °C and post-stained with GelRed (Biotium).
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bioRxiv - Evolutionary Biology 2020Quote: ... After electrophoresis in a 1.5% agarose gel stained with 2% GelRed (Biotium, Hayward, CA, USA), amplicons of the expected size were sequenced on both strands by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... Gels were poststained for 2 hours with polyacrylamide gel electrophoresis (PAGE) GelRed DNA stain (Biotium) and imaged using a Typhoon Imager ...
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bioRxiv - Cell Biology 2023Quote: ... All supernatants were electrophoresed on a 2% agarose gel and stained with Gel Red (Biotium).
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bioRxiv - Cell Biology 2022Quote: ... 5 minutes each with PBS and incubated for 10 minutes at RT with a 1:200 dilution in PBS of the lipophilic dye CellBrite™ (Biotium. Cat. Nº: 30023). Finally ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High-molecular weight genomic DNA was confirmed from these extractions by visualizing each isolation on a 1% agarose gel stained with GelGreen® nucleic acid stain (Biotium, Hayward, CA, USA). Isolation quantity and quality was assessed using a Qubit™ fluorometer and a NanoDrop™ One (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Live stain cells were washed twice using 100 µl ASW and then incubated for 10 min in 50 µg/ml WGA-CF633 conjugate (Biotium, Cat No #29024-1) in ASW ...
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bioRxiv - Plant Biology 2021Quote: ... protonemal cells were stained by the 5 µM of fluorescent dye SynaptoGreen C4 (equivalent to FM1-43, Biotium, USA ...
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bioRxiv - Biochemistry 2020Quote: ... BRET signals were recorded from microsomes (each ∼10 µg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... BRET signals were recorded from microsomes (each ∼10 μg) in the presence of 5 µM coelenterazine (Biotium Corp.) using the Cytation 5 image reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... samples were placed on a microscope slide with 0.8% agarose gel containing 5 μM Bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC4(3)) (Biotium) and 0.75 μM permeability reporter PI (MP Biomedicals) ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR product was run on 2% agarose gel followed by staining with GelRed (Biotium, USA). The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel ...
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bioRxiv - Microbiology 2022Quote: ... The results of the reaction were assessed using a 2% agarose gel stained with GelRed (Biotium). Previous literature has identified this method suffers from low sensitivity ...
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Biochemistry 2021Quote: Polymerase activity from 5°C to 50°C was measured with EvaEZ™ Fluorometric Polymerase Activity Assay Kit (Biotium) in the provided Tris buffer system based on the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... PCR success was checked on a standard 2% agarose gel visualized with GelRed nucleic acid stain (Biotium). Sequencing of successful PCR product was performed by the Psomagen Inc ...
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bioRxiv - Neuroscience 2021Quote: Ratiometric calcium imaging experiments were conducted with the fluorescent indicator Fura-2 (Thermo Fisher Scientific or Biotium). Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplicons from each reaction were visualized on 2% agarose gel stained with RedsafeTM (Biotium, CA, USA). Capillary sequencing was performed using forward and reverse primers with the BIG dye terminator chemistry v3.1 (Applied Biosystems ...