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Citations for Biotium Inc. :
151 - 200 of 205 citations for 7 Hydroxy 2 3 dihydro 1H cyclopenta c chromen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... postsynaptic nicotinic receptors (nAChRs) were labeled using α-Bungarotoxin (BTX) conjugated with CF405 (4 μg/mL; Biotium; cat ##00002) for 45 min in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... was loaded by immersing the tissue in a Tyrode’s solution containing the voltage-sensitive indicator/dye Di-4-ANEPPS (10 µM; Biotium) for at least 30 min at room temperature (20–22 °C) ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was determined using the 2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt (XTT) assay according to the manufacturer’s protocol (Biotium). The assay utilizes the ability of viable cells with active metabolism to reduce the yellow tetrazolium salt to the soluble orange formazan product using mitochondrial dehydrogenase enzymes ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed and incubated for 5 minutes at 37°C with 1X Pre-Staining Solution (MemBrite® Fix 543/560, #30094, Biotium) diluted in DPBS buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... After electrophoresis in a 1.5% agarose gel stained with 2% GelRed (Biotium, Hayward, CA, USA), amplicons of the expected size were sequenced on both strands by Genoscreen (Lille ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR products were visualized on a 2% agarose gel using GelRed™ (Biotium, Freemont, CA) before multiplexing with consideration to dye color and allele size (Table S1) ...
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CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosisbioRxiv - Cell Biology 2023Quote: ... PCR products were electrophorized on 2% agarose gels containing GelRed® (Biotium, Fremont, CA, USA) and images were captured using the ChemiDoc(tm ...
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bioRxiv - Plant Biology 2023Quote: ... PCR products were run in 2 % w/v agarose gels stained with Gel Red (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... Gels were poststained for 2 hours with polyacrylamide gel electrophoresis (PAGE) GelRed DNA stain (Biotium) and imaged using a Typhoon Imager ...
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bioRxiv - Cell Biology 2023Quote: ... All supernatants were electrophoresed on a 2% agarose gel and stained with Gel Red (Biotium).
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bioRxiv - Biophysics 2021Quote: ... The upstream handle was labeled at the 3’ end of the DNA strand by introducing biotin-16-dUTP (Biotium, Fremont, CA, USA) using the T4 DNA polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... at a 1:2000 dilution in PBS with 4% BSA was added and incubated for 30 min together with CF405M-Phalloidin (Biotium) at a 1:100 dilution to label F-actin ...
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bioRxiv - Microbiology 2021Quote: ... at 80°C for 10 minutes in 10−4 final dilution of commercial stock as described earlier (35) or with GelRed nucleic acid gel stain (Biotium,10−4 final dilution of commercial stock ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated at 4°C for 4 h with CF488-conjugated anti-mouse IgG (1:2000) and CF568-conjugated anti-rabbit IgG (1:2000) antibodies (Biotium) diluted in PBS containing 2% FBS as secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... the inserts were transferred to a fresh 24-well plate with black walls containing 500 μl of 4 μM Calcein AM (Biotium) in mHBSS per well ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR product was run on 2% agarose gel followed by staining with GelRed (Biotium, USA). The band size between 137nt–149nt was selected and purified using Gel Extraction Kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2021Quote: ... and qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Microbiology 2022Quote: ... The results of the reaction were assessed using a 2% agarose gel stained with GelRed (Biotium). Previous literature has identified this method suffers from low sensitivity ...
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bioRxiv - Microbiology 2022Quote: ... and qualitatively evaluated by mixing ~2 μL of each sample with 1 μL of GelRed (Biotium), and running it on a 0.7 % agarose gel for 30 minutes at 150 V ...
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bioRxiv - Immunology 2024Quote: ... cell viability was quantified using the ATP-Glo™ Bioluminometric Cell Viability Assay (Biotium #30020-2) on a CLARIOstar plate reader.
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bioRxiv - Zoology 2020Quote: ... PCR success was checked on a standard 2% agarose gel visualized with GelRed nucleic acid stain (Biotium). Sequencing of successful PCR product was performed by the Psomagen Inc ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were run on a 2% agarose gel supplemented with gel green dye (Biotium, 41005-1). A subset of F0 samples was submitted to GeneWiz for Sanger sequencing and analyzed using SnapGene software (SnapGene).
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bioRxiv - Neuroscience 2021Quote: Ratiometric calcium imaging experiments were conducted with the fluorescent indicator Fura-2 (Thermo Fisher Scientific or Biotium). Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were incubated with CF350-conjugated streptavidin for 2 h (1:3000; Biotium, Inc., Fremont, CA) for fluorescent investigation of biocytin filled neurons ...
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bioRxiv - Molecular Biology 2024Quote: ... The amplicons from each reaction were visualized on 2% agarose gel stained with RedsafeTM (Biotium, CA, USA). Capillary sequencing was performed using forward and reverse primers with the BIG dye terminator chemistry v3.1 (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were the run on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA), and visually inspected in UV lighting (Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... the chromatin was stained with RedDot™2 Far-Red Nuclear Stain (Biotium, 1:100 diluted in PBS), and the samples mounted using Vectashield Mounting Medium and analyzed on an FV1000 ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: ... Amplified fragments were visualized in 2% agarose gels stained by GelRed Nucleic Acid Gel Stain (Biotium, Fremont, CA). While amplification of short fragments (~ 570 bp ...
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bioRxiv - Microbiology 2020Quote: ... wells were washed twice with fresh media before CAMHB containing the eDNA stain (EthHD-2 (1 μM; Biotium)) was added to the wells ...
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bioRxiv - Molecular Biology 2023Quote: ... purification of tachyzoites from cell debris by filtration though 3 µm filters and incubation of 2×105 extracellular parasites in 39µl of D10 with or without 30µM PMAxx (Biotium) for 15 min in shaking and darkness ...
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bioRxiv - Neuroscience 2024Quote: ... multi-shank neural probe (NeuroNexus A4×4-3mm-100-125-177-A16, Ann Arbor, MI, USA) pre-coated with DiI (Biotium, Fremont, CA, USA) for fluorescent detection of recording sites was advanced dorsoventrally at 5 µm/s through the IC using a hydraulic micropositioner (Model 650 ...
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bioRxiv - Genetics 2020Quote: ... 6 μl of the PCR product was used for electrophoresis in Tris-Acetate EDTA running buffer at 120V for 45 min on a 2% agarose gel stained with GelRed (Biotium). Experiments were repeated twice to verify the results ...
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bioRxiv - Evolutionary Biology 2020Quote: Aliquots (2μl) of PCR products were run for 20 minutes at 80 volts in 2% agarose gels stained with GelRedTM (Biotium) and visualized in a UV light transilluminator Alpha Imager 3400 Documentation and Analysis System (Alpha Innotech ...
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bioRxiv - Neuroscience 2020Quote: ... USA) and the products were separated through electrophoresis on a 2% agarose gel stained with GelRed (Biotium, Fremont, CA, USA). The wild-type allele generated a single fragment of 460 bp and the mutant allele generated two fragments of 276 and 184 bp.
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bioRxiv - Microbiology 2021Quote: ... and the product was visualized on 2% agarose gels ran at 120V for 20 minutes and stained with GelGreen (Biotium). Primers used for probe amplification can be found in the Key Resource table..
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bioRxiv - Immunology 2021Quote: To measure apoptosis 200 µL/well NucViewTM 530 red solution (2 µM in phenol red free and serum free RMPI medium) (Biotium) was added for 30 min at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were analyzed by separation of mutant and wild-type alleles on a 2% agarose gel stained with Gel Red (Biotium). Primer sequences are listed in Table S4.
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bioRxiv - Genetics 2021Quote: Success of PCR was determined by electrophoresis using a 2 % (w/v) agarose gel stained with GelRed® (Biotium, USA) and visualized under UV light ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 4ul of PCR product was visualised by electrophoresis on a 2% agarose gel stained with GelRed Nucleic Acid Gel Stain (Biotium).
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... BigBlock assemblies and PCR products were analyzed by electrophoresis on 1-2% agarose-TBE gels stained with GelRed® (Biotium).
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bioRxiv - Evolutionary Biology 2023Quote: ... Japan) for 2 h at room temperature and then stained with 1 μM Mito View Green solution (Biotium, Fremont, CA) and 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were analyzed on 2% agarose gel and visualized with the aid of GelGreen nucleic acid gel stain (Biotium).
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bioRxiv - Cell Biology 2024Quote: ... PCR products were identified after agarose gel electrophoresis (500 A/90 V) on a 1.5 % or 2 % agarose gel and staining with GelRed® (41003; Biotium).
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bioRxiv - Microbiology 2020Quote: ... at a final concentration of 20 μg/ml and incubated in the dark for 2 min before detection or with CellBrite™ Fix 488 Membrane Dye (Biotium) at a final concentration of 10X (from a 1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... PCR reaction products were separated on a 2 % agarose gel electrophoresis and visualized with GelRed (nucleic acid gel stain; #41003, Biotium, USA).
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bioRxiv - Cell Biology 2021Quote: At 48h post-infection cells were incubated with 300μl of 4 μM Calcein-AM and 2 μM EthD-III from the Viability/Cytotoxicity Assay Kit for Animal Live and Dead cells (Biotium 30002-T) for 45 min at room temperature as per manufacturer’s recommendations ...
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bioRxiv - Physiology 2023Quote: ... preparations were loaded with Ca2+ (Rhod-2 AM, 50 µL of 1 mg/mL in DMSO + 10% pluronic acid, Biotium, Hayward, CA) and voltage (Vm)-sensitive dyes (RH237 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were separated by electrophoresis through a 2% agarose gel in 1× TBE and stained with GelRed (Biotium, Hayward, CA, USA). To confirm the sequence of each band ...